spermatogonial stem cell
The spermatogonial stem cell niche in testicular germ cell tumors
... Leydig cell-derived IGF-I mediates the maintenance of spermatogonial stem cell pluripotency and confers on them a PGC-like phenotype through PI3K pathway (Huang et ...directional cell ...
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The effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture
... from spermatogonial stem cell clusters ...from spermatogonial stem cell clusters in culture groups (A, D and G) on nanofiber scaffolds (Experimental 1–3) had morphology typical ...
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Spermatogonial stem cell sensitivity to capsaicin: An in vitro study
... The doses of CAP used in our cultures were based on pre- vious reports in other cell types [22,23] and our experi- ence with the spermatogonial stem cell lines. The effect of CAP on testes has ...
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Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice
... with spermatogonial stem cell transplantation (SSCT) has been proposed as a possible fertility preservation strategy for these patients [4, ...
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The Effect of Caffeic Acid on Spermatogonial Stem Cell-type A Cryopreservation
... Spermatogonial stem cell (SSC) purification Supernatants was carefully removed, then the cells were washed with PBS buffer and incubated with ...(34). Spermatogonial stem cells were ...
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An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation
... Background: Spermatogonial stem cell (SSC) transplantation (SSCT) has become important for conservation of endangered species, transgenesis and for rejuvenating testes which have lost germ cells (Gc) ...
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Loss of Gata4 in Sertoli cells impairs the spermatogonial stem cell niche and causes germ cell exhaustion by attenuating chemokine signaling
... expressed genes in Gata4 cKO testes were chemotaxis, cell apoptosis, Sertoli cell transcription factor, cell adhesion and extracellular matrix, fatty acid metabolism and RA metabolism. Notably, the ...
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Colony stimulating factor 1 is an extrinsic stimulator of mouse spermatogonial stem cell self renewal
... Interstitial Leydig cells and select peritubular myoid cells express Csf1 within mouse testes To gain a better understanding of the potential contribution that Csf1 has on SSC niche function, its expression in vivo was ...
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Investigation on the Effect of Low Intensity Ultrasound Stimulation on Mouse Spermatogonial Stem Cell Proliferation and Colonization
... permatogonial stem cells (SSCs) are the foundation of spermatogenesis and male ...specific stem cells, SSCs are rare, representing only ...other stem cells, SSCs defined by their ability to balance ...
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Colony formation ability of frozen thawed spermatogonial stem cell from adult mouse
... spermatogonial cells co-culture with Sertoli cells Taken together, as demonstrated in Tables I and II, our results indicated that number of the colonies and their diameters in the co-culture with fresh cells ...
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A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery
... Several investigators have shown similarities between Sertoli cells and MSC [40], although MSC did not ori- ginate from Sertoli cells, at least not in in vitro experi- ments [41]. Therefore, MSC might support or attract ...
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Testicular tissue cryopreservation and spermatogonial stem cell transplantation to restore fertility: from bench to bedside
... testicular cell suspensions ...other stem cells and cancer cells, especially hematopoietic cells [77]; therefore, the sorting methods have not yielded tumor cell-free ...
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Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation
... Isolation of Sertoli cells from NOA and OA patients Testicular biopsies obtained from patients were washed three times aseptically in Dulbecco modified Eagle medium (DMEM). Sertoli cells were isolated from hu- man testis ...
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Follicle stimulating hormone increases spermatogonial stem cell colonization during in vitro co-culture
... Suitable populations of Sertoli cells and SSCs could be obtained from 3 to 5 months old calves, because the seminiferous epithelium of calves’ testis contains two distinct cell types: type-A SSCs and Sertoli ...
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Live imaging of the Drosophila spermatogonial stem cell niche reveals novel mechanisms regulating germline stem cell output
... of stem cells harboring harmful mutations within the niche, which can compete for niche occupancy (Issigonis et ...with stem cells exhibiting neutral drift dynamics (Klein et ...mammalian stem cells ...
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Cyclical expression of GDNF is required for spermatogonial stem cell homeostasis
... Sertoli cell expression was crucial for ...germ cell overexpression of GDNF made it difficult to discern whether these phenotypes reflected the endogenous role of GDNF in the ...Sertoli cell ...
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Flenkenthaler, Florian (2017): Secretome analysis of testicular peritubular cells as a clue for solving the puzzle of the human spermatogonial stem cell niche. Dissertation, LMU München: Tierärztliche Fakultät
... various cell types (26, 36- 39). The presence of cellular proteins in cell culture supernatants can arise from secretory events or can be the result of cell lysis or ...analyzed cell lysates ...
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Propagation of human germ stem cells in long-term culture
... another spermatogonial stem cells marker was studied in our ...a spermatogonial stem cell specific transcription factor in the testis is required to regulate self- renewal and ...
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Genetic and epigenetic properties of mouse male germline stem cells during long term culture
... retained spermatogonial stem cell activity, we used a spermatogonial transplantation technique that allows competent spermatogonial stem cells to colonize the empty seminiferous ...
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Akt mediates self renewal division of mouse spermatogonial stem cells
... Spermatogonial stem cells have unique properties to self-renew and support spermatogenesis throughout their ...glial cell line-derived neurotrophic factor (GDNF) has recently been identified as a ...
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