Sphingosine-1-phosphate receptor
Sphingosine 1 phosphate receptor 1 reporter mice reveal receptor activation sites in vivo
... GPCR sphingosine-1-phosphate receptor 1 (S1P1) by sphingosine-1-phosphate (S1P) regu- lates key physiological ...of receptor activation during normal ...
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HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver
... endothelial sphingosine-1- phosphate receptor-1 (S1P 1 ) by its natural ligand bound to HDL (HDL-S1P) induces liver regeneration and curtails ...S1P 1 in the ...S1P ...
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Enhanced sphingosine 1 phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility
... (Table 1 and Supplemen- tal Table 1; supplemental material available online with this arti- cle; ...IL-20 receptor b, a receptor associated with skin autoimmune diseases (34–37), and ...
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<p>Nanoparticle BAF312@CaP-NP Overcomes Sphingosine-1-Phosphate Receptor-1-Mediated Chemoresistance Through Inhibiting S1PR1/P-STAT3 Axis in Ovarian Carcinoma</p>
... STAT3-induced sphingosine-1-phosphate receptor-1 (S1PR1), a G protein-coupled receptor for sphingosine-1-phosphate (S1P), is crucial for persistent STAT3 ...
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Selectivity and specificity of sphingosine 1-phosphate receptor ligands : "off-targets" or complex pharmacology?
... S1P receptor ligand does not immediately invalidate the conclusion ...of receptor ligands is also required to add power to the validation process and one should be aware of limitations in interpretation of ...
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Transcriptional targeting of sphingosine-1- phosphate receptor S1P2 by epigallocatechin- 3-gallate prevents sphingosine-1-phosphate- mediated signaling in macrophage-differentiated HL-60 promyelomonocytic leukemia cells
... DNA amplification was carried out using an iCycler iQ™5 (Bio-Rad Laboratories), and product detection was performed by measuring binding of the fluorescent dye SYBR Green I to double-stranded DNA. The following ...
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The sphingosine 1-phosphate receptor 2 is shed in exosomes from breast cancer cells and is N-terminally processed to a short constitutively active form that promotes extracellular signal regulated kinase activation and DNA synthesis in fibroblasts
... the receptor has a role in regulating inter-cellular communication between cancer cells and fibroblasts and whether it can enhance metastatic ...protein 1 (LMP1) and are able to induce ERK and AKT ...
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Essential role of sphingosine 1–phosphate receptor 2 in pathological angiogenesis of the mouse retina
... Animals. C57BL/6 × 129Sv mice with targeted disruption of the S1p2 gene were generated as previously reported (13). Mice were maintained on a mixed C57BL/6 × 129Sv genetic background, and experiments on KO mice were ...
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Sphingosine 1-phosphate receptor 1 signaling in mammalian cells
... lipid, sphingosine 1-phosphate (S1P), is produced by phosphorylation of sphingosine, catalysed by two isoforms of sphingosine kinase (SK1 and SK2), whereas its degradation involves ...
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Requirement for sphingosine 1–phosphate receptor 1 in tumor angiogenesis demonstrated by in vivo RNA interference
... containing multiplex siRNA with or without FGF-2 was injected subcuta- neously into athymic nude mice as described. The Matrigel plugs were harvested 7 days after implantation, fixed, sectioned, and H&E-stained as ...
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Sphingosine-1-phosphate receptor inhibition prevents denervation-induced dendritic atrophy
... 4352932E; sphingosine-1-receptor 1 (S1PR1) Mm00514644_m1eas; sphingosine-1-receptor 3 (S1PR1R3) Mm02620181_s1 from Applied Biosystems, USA] with a standard amplification ...
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Sphingosine 1-phosphate receptor 3 and RhoA signaling mediate inflammatory gene expression in astrocytes
... p-nitrophenyl phosphate, and phenyl- methylsulfonyl ...at 1:500 dilution, and the band running at 72 kDa band was ...at 1:1000 dilution, and a band at 37 kDa was quanti- ...at 1:4000 dilu- ...
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Effect of sphingosine kinase modulators on interleukin-1β release, sphingosine 1-phosphate receptor 1 expression and experimental autoimmune encephalomyelitis : FTY720 analogues and inflammation
... IL121 receptor associates with SK2 and increases IL-12-induced STAT4-mediated transcriptional activity while dominant negative SK2 in Th1 cells reduced IL-12 stimulated IFN formation and WT SK2 enhanced ...
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Sphingosine 1-phosphate receptor 5 mediates the immune quiescence of the human brain endothelial barrier
... nology, Santa Cruz, CA, USA and Imgenex, San Diego, CA, USA). Subsequently, sections were incubated with EnVision + Dual Link (DAKO, Glostrup, Denmark) according to the manufacturer’s description. Diaminoben- zidine ...
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Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth : the role of sphingosine 1-phosphate receptor-4
... Nuclei Preparation--Cells were scraped in 4 ml of phosphate buffered saline (PBS). The cell suspensions were transferred into 15-ml centrifuge tubes and pelleted at 700×g for 5 min. Cell nuclei were isolated using ...
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Sphingosine kinase 1 and sphingosine 1 phosphate receptor 2 are vital to recovery from anaphylactic shock in mice
... kinase 1 (SphK1) and SphK2 are ubiquitous enzymes that generate sphingosine-1-phosphate (S1P), a ligand for a family of G protein–coupled receptors (S1PR1–S1PR5) with important functions in ...
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Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1
... cells were seeded in a 6-well plate in 1 ml of media for 24 hours. Media was collected, centrifuged and pure supernatant was analyzed. Media was diluted 1:5 prior to assay and IL-6 concentration was ...
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Effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to FTY720 during neuroinflammation
... Immunoblot analysis. Splenocytes from MOG 35-55 -immunized WT and S1PR1(S5A) mice treated with FTY720 (0.5 mg/kg, daily i.p. injections from day 0 to 8) were activated in vitro with MOG 35-55 (10 μg/ ml) at 37 °C for 30 ...
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Sphingosine kinase 1 as an anticancer therapeutic target
... Elevated sphingosine kinase 1 (SK1) levels is predictive of a poor prognosis, and SK1 overexpression may confer resistance to ...(S1P)/sphingosine-1-phosphate receptor (S1PR) ...
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Crystal structure of sphingosine kinase 1 with PF-543
... of sphingosine kinase inhibitors have been ...of sphingosine and show only micromolar a ffi ...phosphorylating sphingosine 19 and is itself phosphorylated by SPHK2 causing it to act as a functional ...
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