SPHK1/2
Nondestructive Evaluation Of Corrosion Damage In Reinforced Concrete Aged Slab Specimen
... metabolism via sirtuin 1 (SIRT-1) and poly ADP-ribose polymerase 1 (PARP-1) pathways, carcinogenesis through the inactivation of cyclooxygenase (COX) proteins, and the phosphorylation of sphingosine by sphingosine kinase ...
37
Leucine-rich Repeats and Immunoglobulin 1 (LRIG1) Ameliorates Liver Fibrosis and Hepatic Stellate Cell Activation via Inhibiting Sphingosine Kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) Pathway
... LX- 2 cells were increased and SphK1, α-SMA, Col1α1, TIMP-1, Fn-1, TGF-β1 contents were decreased after TGF-β treatment, while there were opposite effects by overexpression of ...
12
An novel role of sphingosine kinase 1 (SPHK1) in the invasion and metastasis of esophageal carcinoma
... genes, SPHK1, was of particular interest to our ...of SPHK1 up-regulated MMP1 mRNA and promoter activity as well as its protein levels [12], and the MMP1 gene is known to play a very important role in the ...
15
Original Article SphK1 is involved in the progression of human pancreatic cancer
... of SphK1 on Ser225, cause a rapid activation of SphK1 ...of SphK1 and induce translocation of SphK1. SphK1 is a cytosolic enzyme, which is translocation to the plasma membrane [22], ...
7
Crystal structure of sphingosine kinase 1 with PF-543
... Protein Expression and Puri fi cation. The construct DNA was used to prepare recombinant baculovirus, which was used to infect Sf 9 cells at a density of 2 million cells/mL in glass conical flasks. The flasks were ...
5
Identification of SPHK1 as a therapeutic target and marker of poor prognosis in cholangiocarcinoma
... against SPHK1 (rabbit anti-SPHK1 antibody, ab61148, Abcam, Cambridge, UK) was diluted (1:200) and added to the slides that were then incubated overnight at ...(mild), 2 (moderate), or 3 ...
15
TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway
... TRIM14 short hairpin RNAs (shTRIM14) or con- trol shRNA (shNC) was constructed in PLKO.1 vector (Addgene, Cambridge, MA, USA). The cDNA encod- ing full-length human TRIM14 was constructed in PLVX-puro Vector (Clontech, ...
9
Lentivirus-mediated siRNA knockdown of SPHK1 inhibits proliferation and tumorigenesis of neuroblastoma
... of SPHK1 using the BeyoFast™ SYBR Green qPCR Mix Kit (Beyotime, Haimen, Jiangsu, People’s Republic of China) at an ABI 7300 Real-Time PCR System (Applied Biosystems, Life Technologies GmbH, Darmstadt, ...step ...
10
Correlation of SphK1 and FAK expression in colon cancer tissue with clinical pathological features and epithelial-mesenchymal transition
... that SphK1 and FAK gene expression in colon cancer tissue were positively correlated with Lin28A, PRDX1, PRM1 and SLP-2 mRNA expression and negatively correlated with ZNRD1 mRNA expression, indicating that ...
5
Activation of the SPHK/S1P signalling pathway is coupled to muscarinic receptor-dependent regulation of peripheral airways
... Our findings strongly suggest that SPHK1 activation is part of the signalling response to M 2 R stimulation in peripheral mouse airways. These airways resemble in their structure and composition of cell ...
14
HJURP Promotes Epithelial-to-Mesenchymal Transition via Upregulating SPHK1 in Hepatocellular Carcinoma
... Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide[1]. According to some epidemiologic studies, around 780,000 new patients were diagnosed worldwide in 2012 and nearly 750,000 cases of ...
9
MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1
... and SphK1 (up-regulated) that we observed appeared to be definitive in the two high-metastasis potential ovarian cancer cell lines, SKOV3-ip and ...of SphK1 and ...
9
Improved Simultaneous Estimation of Location and System Reliability via Shrinkage Ideas
... Figure 2.1 Methylene-blue stained mouse skin for MC detection and analysis ... 30 Figure 2.2 Mass descriptors distinguish intact from degranulated MC ................ 31 Figure 2.3 Fractal dimension (D) independently ...
138
Emerging role of sphingosine-1-phosphate signaling in head and neck squamous cell carcinoma
... factor 2, an E3 ubiquitin ligase that is a key component of the nuclear factor (NF)- κ B ...Both, SphK1 and the production of S1P are necessary for TNF-mediated NF- κ B ...
12
Sphingosine kinase 1 expression enhances colon tumor growth
... for SphK1 and COX-2 was conducted using rabbit anti-human SphK1 antibody (AP7237c; 1:100 dilution in blocking buffer, Abgent, San Diego, CA) and Rabbit anti-COX-2 anti- body (CRM 306 A; 1:100 ...
8
Sphingosine kinase1 : a potential therapeutic target in pulmonary arterial hypertension?
... Pulmonary arterial hypertension (PAH) affects 15-50 people/million and is characterised by elevated mean arterial blood pressure, shortness of breath, fatigue, dizziness, chest pain and edema. PAH can lead to reduced ...
36
Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1
... Figure 2 SphK1 regulates EGFR and ...in SphK1 overexpressing cells compared to mock conditions ...(B) SphK1 transient knockdown reduced EGF-induced EGFR, STAT3, and ERK phosphorylation ...by ...
9
Erythrocyte derived sphingosine 1 phosphate is essential for vascular development
... and 2 (Sphk1 and Sphk2) showed embryonic lethality between ...rbc-specific Sphk1 Sphk2–KO embryos were anemic, the erythropoietic capacity of hematopoietic stem cells (HSCs) was not impaired, ...
7
Sphingosine kinase 1 is a potential therapeutic target for nasopharyngeal carcinoma
... of SPHK1 in NPC cells, we used FTY720 (also known as fingolimod), which can be used as a SPHK1 inhibitor [20], to block the endogenous activity of SPHK1 in ...of SPHK1 enzymatic activity in ...
13
Sphingosine kinase 1 enables communication between melanoma cells and fibroblasts that provides a new link to metastasis
... from SPHK1 expressing COLO829 cells (compared with COLO829 cells, SPHK1 null), Albinet et ...from SPHK1 expressing COLO829 cells also increased the expression of SPHK1 in fibroblasts and this ...
10