Sputum Sample
Occurrence of N Acyl Homoserine Lactones in Extracts of Bacterial Strain of Pseudomonas aeruginosa and in Sputum Sample Evaluated by Gas Chromatography–Mass Spectrometry
... different AHLs were separated and identified in the spiked samples by GC-MS without any chemical mod- ification. Five of these AHLs were identified in real sample of P. aeruginosa culture and two AHLs were found ...
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Significant roadblocks exist in developing sputum sample libraries for clinical validation of novel in vitro diagnostics
... sputum sample library is a major roadblock to registration of next-generation ...a sputum library, Figure 2 illustrates that since there is no simple solution to storage of sputum samples for ...
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Mixed infection and clonal representativeness of a single sputum sample in tuberculosis patients from a penitentiary hospital in Georgia
... Consistently, respective isolates from the 18 patients with major IS6110 band differences were all found to have very distinct MIRU-VNTR profiles as well, corroborating the conclusion of mixed infection. More detailed ...
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PHYTOCHEMICAL AND ANTIMICROBIAL EVALUATION OF Cnidoscolus aconitifolius LEAF EXTRACT AND HONEY AGAINST Klebsiella pneumoniae, Streptococcus pneumoniae ISOLATED FROM SPUTUM SAMPLE
... positive like Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus and Gram negative like Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter cloacae, Haemophilus influenzae, Legionella ...
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Detection of viable but non culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study
... a sputum sample spiked with a mid-log phase culture of ...a sputum sample spiked with a 1:10 mixture of live and dead ...Each sample was divided into two ali- quots, of which one was ...
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Diagnosing tuberculosis in hospitalized HIV infected individuals who cannot produce sputum: is urine lipoarabinomannan testing the answer?
... a sputum sam- ple for diagnostic ...– sputum-scarce patients present a significant challenge to clinicians and TB control pro- grams alike, and highlight deficiencies in current diag- nostic algorithms that ...
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Comparison of loop mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis
... the sputum sample and vortex mixed in a class I BSC to decon- taminate the ...decontaminated sputum sample was transferred into a centrifuge tube using sterile 1 ml pasture pipette and ...
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Sensitivity of direct versus concentrated sputum smear microscopy in HIV infected patients suspected of having pulmonary tuberculosis
... randomly-timed sputum sample for direct smear micros- copy at the time of ...early-morning sputum sample on the morning following admission; this sample was sent for both direct and ...
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Evaluation of Increased Sensitivity of Morning Bleach Sample for Detection of Acid Fast Bacilli in Pulmonary Samples
... if sputum is liquefied with one or another chemical reagent and there after concentrated with centrifugation prior to acid fast staining [6] ...in sputum specimen, thereby improving case finding of ...
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Insufficient quality of sputum submitted for tuberculosis diagnosis and associated factors, in Klaten district, Indonesia
... the sputum sample and the quality of the microscopic examination ...of sputum and factors related to ...per sputum slide ...good sputum sample, and attitude and compliance with ...
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Multilocus Sequence Typing Scheme versus Pulsed Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates
... break 5 isolates were found to belong to ST22, and their PFGE profiles are indistinguishable from that of isolate IAL 063, which was obtained from the sputum sample of a patient in a dif[r] ...
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lytA Quantitative PCR on Sputum and Nasopharyngeal Swab Samples for Detection of Pneumococcal Pneumonia among the Elderly
... serology, sputum, NPS, and urine, as well as the assays carried out to detect pneumococci, have been described in detail previously ...The sputum samples were spontaneously produced or induced with a NaCl ...
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Development of Algorithm Tuberculosis Bacteria Identification Using Color Segmentation and Neural Networks
... The examination of TB bacteria all this time is done manually, so that is required a long time enough and trained laboratory staff. Therefore in this research is developed system which can detect TB bacteria by using ...
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Molecular Detection of Mixed Infections of Mycobacterium tuberculosis Strains in Sputum Samples from Patients in Karonga District, Malawi
... Other important differences lie in the laboratory methods. In our study, DNA was extracted directly from processed spu- tum samples, while in the South African study, sputum samples were first cultured in Bactec ...
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Comparison of Protein Profiles in Sputum between COPD and Acute Exacerbation of COPD
... the sputum were se- lected carefully and placed in a preweighed Eppendorf tube, to which four volumes of ...homogenized sputum, and the total cell count was determined with a ...mogenized sputum was ...
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Improving the Sensitivity of the Xpert MTB/RIF Assay on Sputum Pellets by Decreasing the Amount of Added Sample Reagent: a Laboratory and Clinical Evaluation
... (iii) Relationship between Xpert results and time to positiv- ity in liquid culture. The above-mentioned results suggest that the 2:1 SR treatment ratio would be most beneficial in sputum samples with low M. ...
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Immuno diagnosis of Mycobacterium tuberculosis in sputum, and reduction of timelines for its positive cultures to within 3 h by pathogen specific thymidylate kinase expression assays
... patient sputum sample (1) several study aliquotes were prepared by dissolving 1 µl of each sputum-dilution in 1000 µl of freshly prepared phosphate buffered ...
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Comparison of Sensitivity and Specificity of ZN and Fluorescent Stain Microscopy with Culture as Gold Standard
... and sputum concentration may also improve sensitivity of ...Morning sputum specimen of patients, suspected of hav- ing pulmonary tuberculosis, over a period of 6 months was subjected to direct microscopy ...
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Prospective multicenter randomized patient recruitment and sample collection to enable future measurements of sputum biomarkers of inflammation in an observational study of cystic fibrosis
... with sputum sample collections in- cluding demographics, CFFPR identification numbers, home address, number and dates of CF exacerbations in the year prior to enrollment, other lung disease, smok- ing, ...
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PrimeStore MTM and OMNIgene sputum for the preservation of sputum for Xpert MTB/RIF testing in Nigeria
... other sample was used for testing the samples stored in Primestore and Omnigene ...the sputum sample, collecting between 0.1 and 0.2 mL of sputum; placed in the Primestore collection tube; and ...
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