SSU rRNA gene
Evolution of small putative group I introns in the SSU rRNA gene locus of Phialophora species
... Previously, the small intron (PaSSU) from P. americana Wang 1046 was demonstrated to splice in vivo [14], has group I ribozyme activity in vitro, and phylogenetically falls within the IC1 subgroup [13]. All of the ...
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Towards a balanced view of the bacterial tree of life
... metagenomic SSU rRNA gene sequences, our survey re- veals the relative contributions of established phyla to the overall taxonomic richness within the domain bac- teria ...our SSU rRNA ...
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Molecular Phylogenies of Blastocystis Isolates from Different Hosts: Implications for Genetic Diversity, Identification of Species, and Zoonosis
... full-length SSU rRNA (1, 7, 29, 43, 49, 50) and elonga- tion factor 1 ␣ (EF-1 ␣ ) gene sequences ...Blastocystis SSU rRNA-based analyses with those of protein-coding ...full-length ...
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Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan
... b gene sequence of the parasite was determined and analyzed with the BLASTn ...and SSU rRNA genes were amplified from the flagellate and the sequences were analyzed since these genes have been well ...
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Survey and molecular characterization of trichomonads in pigs in Anhui Province, East China
... scopic examination (18, 19). Therefore, sensi- tive and specific molecular methods are now used widely to identify trichomonad species and strains (20-22). Although several porcine trichomonad species have been ...
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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
... the SSU rRNA gene amplification reactions were also used to visu- alise the DNA fragments obtained from the PCR and nested ...GP60 gene using the primers described by Peng et ...
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Molecular detection of human Plasmodium species in Sabah using PlasmoNex™ multiplex PCR and hydrolysis probes real time PCR
... Plasmodium 18S ssu rRNA gene of all five human mal- aria parasites [10], (2) a multiplex PCR for the detection of three Plasmodium species, i.e., P. falciparum , P. vivax , and P. Malariae, using ...
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Trypanosoma livingstonei: a new species from African bats supports the bat seeding hypothesis for the Trypanosoma cruzi clade
... the SSU rRNA gene for barcoding trypanosomes has demonstrated that this sequence is sufficiently polymorphic to distinguish all species from the several vertebrate classes examined to date ...V7V8 ...
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Multigene-Based Analyses of the Phylogenetic Evolution of Oligotrich Ciliates, with Consideration of the Internal Transcribed Spacer 2 Secondary Structure of Three Systematically Ambiguous Genera
... Oligotrichs are ciliates of great abundance, but their molecular systematics are rarely studied. In this study, nine species repre- senting three genera (Strombidium, Novistrombidium, and Omegastrombidium) of marine ...
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New type of SSUrDNA sequence was detected from both Plasmodium ovale curtisi and Plasmodium ovale wallikeri samples
... SSU rRNA gene was the most widely accepted mo- lecular marker in identifying different malaria parasites infecting humans with PCR ...on SSU rDNA sequences were NP-1993 ...variant SSU ...
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Revisiting the infectivity and pathogenicity of Cryptosporidium avium provides new information on parasitic sites within the host
... Results: Results showed that no Cryptosporidium oocysts were detected in the feces of experimentally inoculated BALB/c mice, Mongolian gerbils, quail or budgerigars within 30 days post-infection (dpi). Oocysts were first ...
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How many novel eukaryotic 'kingdoms'? Pitfalls and limitations of environmental DNA surveys
... no SSU rRNA sequences from jakobid flagellates were available, and data on other so-called excavate taxa were ...first SSU rRNA gene sequence from the heliozoan-like Sticholonche ...
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“Genetic divergence in Mugil cephalus and Liza ramada based on PCR–RFLP analysis of mtDNA segments” by Heba A.M. Abd El-kader, Aboelhassan M. D., Karima, F. Mahrous, Egypt.
... 16S rRNA mitochondrial gene between two mullet species ...(12s rRNA, 16s rRNA, and COI) mtDNA ...mtDNA gene segments evidenced that ...
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Genotyping Encephalitozoon hellemIsolates by Analysis of the Polar Tube Protein Gene
... sequence analysis of the ITS, which yielded two genotypes. In fact, the typing resolution at the ITS locus was even lower than the sequence analysis of SSU rRNA, which divided the 24 isolates into three ...
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Genomics of the Uncultivated, Periodontitis-Associated Bacterium Tannerella sp. BU045 (Oral Taxon 808)
... containing rRNA genes. The rRNA-containing contigs were used for a BLAST (38) search of the NCBI refseq_genomic database, restricted to bacterial ...16S rRNA gene matched with greater than 99% ...
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Plasmodium malariae and Plasmodium ovale infections in the China–Myanmar border area
... Malaria epidemiology at the China–Myanmar bor- der has shifted to P. vivax predominance, while the two minor parasite species P. malariae and P. ovale spp. also occurred at very low prevalence. However, these minor ...
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Direct and Rapid Detection by PCR ofErysipelothrix sp DNAs Prepared from Bacterial Strains and Animal Tissues
... swine on the basis of a clinical diagnosis, it is important for detection purposes to distinguish among the four species of Erysipelothrix as well as other causative bacterial pathogens. In this study, we have ...
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Use of a Multiplex Molecular Beacon Platform for Rapid Detection of Methicillin and Vancomycin Resistance in Staphylococcus aureus
... Drug resistance, particularly vancomycin and methicillin resistance, in Staphylococcus aureus continues to emerge as a significant public health threat in both the hospital and community settings. In addition to the ...
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Molecular Identification of GemellaSpecies from Three Patients with Endocarditis
... biochemical tests, as described for patient 3 of this study. This method is quick and cheap, but it requires that a bacterium be easily subcultured. If this is not possible, additional sequencing can be used in order to ...
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Catheter associated bacteremia by Mycobacterium senegalense in Korea
... 16S rRNA gene, rpoB gene, and ITS region. By 16S rRNA gene, rpoB gene, and ITS sequence analyses, we concluded that an agent of CVC infection in our patient was ...rpoB ...
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