stable cell lines
Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production
... a cell is most readily examined in the context of a cell and, more pertinently, a virus ...in cell-free systems has lagged behind our success with soluble ...a cell offers a quantitative and ...
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Identification of Plk4 interacting partners and establishment of Plk4 stable cell lines.
... holes within the cytoplasm. Prolonged Zeocin exposure (~ 7-10 days) caused any sensitive cells to eventually disintegrate due to a complete breakdown o f cellular structures such as the endoplasmic reticulum, Golgi ...
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A monoclonal antibody raised against a thermo stabilised β1 adrenoceptor interacts with extracellular loop 2 and acts as a negative allosteric modulator of a sub set of 1 adrenoceptors expressed in stable cell lines
... room temperature. Images show the localisation of mAb3 staining (upper panels) and Hoechst 33342 nuclear stain (lower panels). Scale bar is 20 l m and applies to all panels. Images were obtained from a single experiment ...
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Essential Roles of mTOR/Akt Pathway in Aurora-A Cell Transformation
... We have previously shown that MMTV-Aurora-A transgene developed mammary gland tumors that expressed phosphorylated mTOR and Akt (11). To study the mechanism of how Akt pathway is activated by Aurora-A, we established ...
7
MiRNA-145 increases therapeutic sensibility to gemcitabine treatment of pancreatic adenocarcinoma cells
... Stable cell lines over-expressing miR-145 or negative control miRNA (miR-NC) were established using lentivirus carrying miR-145 or miR-NC, and verified by detecting the expression of miR-145 levels ...
12
Deleterious Effects of Hepatitis Delta Virus Replication on Host Cell Proliferation
... of stable cell lines or transgenic mice which continuously replicate HDV, we have been unable to observe persistent HDV replication following introduction of a ...the cell lines, though ...
5
Robust Production of Infectious Hepatitis C Virus (HCV) from Stably HCV cDNA-Transfected Human Hepatoma Cells
... the stable cell lines or in the infected naı¨ve ...any cell death, lytic infection, or cytopathic ...in cell morphology either in the stable cell lines or in the ...
11
miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation
... C2C12 stable cell lines expressing a GFP-tagged version of Srsf9 fused to a heterologous 3′ UTR containing the SV40 polyA signal as well as GFP only or empty vector ...of stable transfec- ...
12
Utilization of Site-Specific Recombination in Biopharmaceutical Production
... Suitable stable cell lines are widely generated for high-level expression of recombinant proteins using random integration linking genomic amplification; however, it is very laborious and ...
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The Kaposi's Sarcoma-Associated Herpesvirus K5 E3 Ubiquitin Ligase Modulates Targets by Multiple Molecular Mechanisms
... the cell lines and examined by Western blotting, revealing equivalent expression levels of each of the mutants, comparable to wild-type K5 ...the stable cell lines were stained for ...
11
Hypoxia-inducible factor 1 alpha is required for the tumourigenic and aggressive phenotype associated with Rab25 expression in ovarian cancer
... Whole cell extracts were analyzed by Western blot for VEGF and vimentin ...in cell number were observed following transfection of either pcDNA3 or pcDNA-Rab25 ...A2780-Rab25 stable cell ...
15
Stable 293 T and CHO cell lines expressing cleaved, stable HIV 1 envelope glycoprotein trimers for structural and vaccine studies
... Both lines are there- fore stable and not prone to genetic instability (Figure ...CHO cell clones, respectively (Figure 2B). Cell viability (parental ...vs. stable cell ...
14
APOBEC3G Contributes to HIV-1 Variation through Sublethal Mutagenesis
... transfecting stable A3G-expressing cell lines (or 293T controls) with either Vif ⫺ or Vif ⫹ HIV-1 vectors along with a VSV-G protein expression ...construct. Cell culture supernatants were ...
9
Elucidation of signaling pathways regulating ribosomal protein S6 kinases
... the stable cell lines, metabolically labelled with ^S]Methionine/Cysteine, was analysed by high-resolution ...the stable cell ...these cell lines by DNA microarray ...
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Distinct Domains of the Influenza A Virus M2 Protein Cytoplasmic Tail Mediate Binding to the M1 Protein and Facilitate Infectious Virus Production
... in stable cell lines and from recombinant ...production, stable MDCK cell lines were generated that ex- pressed M2 proteins (influenza A/Udorn/72 virus strain) with C-terminal ...
12
Inhibition of Viral Replication by Ribozyme: Mutational Analysis of the Site and Mechanism of Antiviral Activity
... from cell lines expressing ribozymes to cleave oligoribonucleotide substrates in ...ribozyme-expressing cell lines contained the expected endonuclease activities ...a cell line ...
9
Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2
... transgenic cell lines were able to stably express up to 2 × 10 2 -fold higher levels of the SCARB2 mRNA compared to the original cell lines (Figure ...three stable cell ...
11
Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment
... H2AX plays a facilitative role in HR and has been shown to participate in RAD51-mediated suppression of DSBs generated in absence of ATR [23]. Furthermore, it was reported that retention of γH2AX foci 24 hours ...
15
P53 mutation in acute T cell lymphoblastic leukemia is of somatic origin and is stable during establishment of T cell acute lymphoblastic leukemia cell lines
... T cell acute lymphoblastic leukemia (T-ALL) were screened for mutations of the p53 tumor suppressor ...B cell line (HABL) was established. Leukemic T cell lines (HATL) were concurrently ...
8
An investigation of the biochemical interaction between subunits of the ATP-sensitive potassium channel
... the cell walls of some strains of this bacterium, which binds to the Fc region of antibodies of several different types of immunoglobulin (Ig) subclass (Kessler ...
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