Stable transgenics with neuron specific expression
Expression and regulation of neuron specific enolase
... gene expression, however, whilst endogenous mRNA accumulated in postnatal mice, the reporter transcript remained at embryonic ...gene expression in postnatal ...of expression, with different ...
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Arsenic, cadmium and neuron specific enolase (ENO2, γ-enolase) expression in breast cancer
... Methods Immunostaining for ENO1 and 2 in normal breast Tissue sections for the immunohistochemical analysis of ENO1 and 2 expression in human breast were obtained from archival paraffin blocks that originated from ...
12
Modulating the expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics
... tissue- specific secretion of ligands from different signaling ...wnt3 transgenics with either tissue-specific expression of an EGFP reporter or a functionally active fusion protein, ...
13
Candida albicans Biofilms: a Developmental State Associated With Specific and Stable Gene Expression Patterns
... bridization of macroarrays were carried out as previously described (19). Each RNA sample was independently labeled and hybridized a minimum of three times against swapped filters. Preliminary normalization, logarithmic ...
10
Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics
... co- expression of introduced genes, the ability to transfer large segments of DNA with minimal rearrangements and lower cost of experimentation (Hiei et ...
11
Serum neuron specific enolase – impact of storage and measuring method
... relatively stable during long term storage, and second that there was a consistent and sig- nificant difference between the LIAISON®NSE and the NSE Cobas e601 method, with the latter showing consist- ently higher ...
7
5 HTr Expression in Primary Trigeminal Neuron Following Injury
... The RT-PCR assay kinetic reliability was demon- strated by detecting the 18S rRNA transcripts at the same level in all samples. Analysis with a housekeeping gene was used to verify the amount of cDNA in the RT reac- ...
9
A relationship between behavior, neurotrophin expression, and new neuron survival
... Singing induced a 30% increase in BDNF protein in HVC. Although this increase seems modest when compared with the 3-fold increase in BDNF mRNA, a few caveats are in order. First, we do not know when the singing-induced ...
6
Neuron specific reduction in CuZnSOD is not sufficient to initiate a full sarcopenia phenotype
... CuZnSOD expression in neuronal tissue with no change in CuZnSOD expression in other tissues ...a neuron specific loss of CuZnSOD and normal levels of muscle CuZnSOD, giving us a model to test ...
31
Blood-retina barrier failure and vision loss in neuron-specific degeneration
... ical expression of the tight junction component claudin5; and (e) cone loss and visual function decline were associated with the presence and severity of the BRB ...
18
Serum and Cerebrospinal Fluid Neuron Specific Enolase for Diagnosis of Tuberculous Meningitis
... heat stable mycobacterial antigen in cerebrospinal fluid by Dot- Immunobinding ...M. Neuron-specific enolase, nucleo- tides, nucleosides, purine bases, oxypurines and uric acid concen- trations in ...
5
Polarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoforms
... SUMMARY Alternative splicing (AS) is an important source of proteome diversity in eukaryotes. However, how this affects protein repertoires at a single-cell level remains an open question. Here, we show that many 3 0 ...
14
Fabrication of Immunosensor Based on Au-silica Nanocomposite for Neuron-specific Enolase Detection
... electrochemical sensors should consider the preparation time in practical application development. In our case, a 60 min incubation period can be used for applications in the clinic. The anti-NSE antibody in the ...
9
Glucocorticoids exacerbate obesity and insulin resistance in neuron specific proopiomelanocortin deficient mice
... POMC expression to create a de facto neuronal POMC deficiency exacerbated the development of obesity, largely via glucocorticoid modulation of appetite, metabolism, and energy ...
12
PROGNOSTIC VALUE OF NEURON SPECIFIC ENOLASE IN SERUM FOR OUTCOME OF INFANTS WITH BIRTH ASPHYXIA
... Neuron specific enolase belongs to the family of enolase enzymes, present in all tissues and organisms capable of ...and stable in biological fluids and its measurement is not influenced by ...
7
Latency-Associated Transcript (LAT) Exon 1 Controls Herpes Simplex Virus Species-Specific Phenotypes: Reactivation in the Guinea Pig Genital Model and Neuron Subtype-Specific Latent Expression of LAT
... LAT expression in certain neuronal cell ...the stable LAT intron, and the 3 ⬘ end of the ICP0 coding region in the previously described HSV species-specific latency ...
9
Developmental and Activity-Dependent miRNA Expression Profiling in Primary Hippocampal Neuron Cultures
... miRNAs expression by prolonged changes in global network activity Homeostatic synaptic plasticity is a negative feedback mecha- nism that neurons use to offset excessive excitation or inhibition by adjusting their ...
13
Worms With a Single Functional Sensory Cilium Generate Proper Neuron-Specific Behavioral Output
... the expression of DAF-19C in those neurons, we coexpressed a transcrip- tional DsRed fusion of the direct DAF-19C target cilia gene ...gfp-positive neuron was then scored for the induction of che-13 T ...
11
Facilitating Neuron-Specific Genetic Manipulations in Drosophila melanogaster Using a Split GAL4 Repressor
... ABSTRACT Efforts to map neural circuits have been galvanized by the development of genetic technologies that permit the manipulation of targeted sets of neurons in the brains of freely behaving animals. The success of ...
10
Rare paraneoplastic erythroderma associated with ectopic neuron specific enolase deposition in basal cells
... Strong expression of neuron-specific enolase (NSE, 2+ positive) was found in both primary cancer and basal cells of the preoperative ...negative expression of ...positive expression of ...
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