Strand break repair
Incorporation of Large Heterologies Into Heteroduplex DNA During Double-Strand-Break Repair in Mouse Cells
... and repair of large (⬎1 kb) insertion/deletion (I/D) heterologies during double-strand-break repair (DSBR) was investigated using a gene-targeting assay that permits efficient recovery of ...
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Evidence for conservative (two-progeny) DNA double-strand break repair.
... Intramolecular double-strand break repair in the RecE pathway (in a recBC sbcA strain): As our aim is in demonstration of intramolecular recombination and, therefore, cons[r] ...
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DNA Double Strand Break Repair and its Association with Inherited Predispositions to Breast Cancer
... DNA repair mechanisms that include DNA mismatch repair, nucleotide excision repair, base excision repair and DNA double strand break ...
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Differential Usage of Alternative Pathways of Double-Strand Break Repair in Drosophila
... double-strand break at a specific location such that expression of the red fluorescent protein, DsRed, in the next generation can be used to infer the frequency at which each pathway was ...conversion ...
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Coordination of DNA Ends During Double-Strand-Break Repair in Bacteriophage T4
... double-strand-break repair (DSBR) proposes that each end of a double-strand break (DSB) is repaired independently by initiating extensive semiconser- vative DNA replication after ...
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Double-strand break repair processes drive evolution of the mitochondrial genome in Arabidopsis
... permits repair of a DSB at stretches of homology greater than 550 bp following heteroduplex for- mation, assuming a break-induced replication model (see Additional file 4, Figure ...
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Mechanisms of Double-Strand-Break Repair During Gene Targeting in Mammalian Cells
... In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin m-locus in a murine hybridoma was examined. The gene-targeting assay ...
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A Role for DNA Mismatch Repair Protein Msh2 in Error-Prone Double-Strand-Break Repair in Mammalian Chromosomes
... Following strand displacement, the theless, our current and previous work illustrate that nascent DNA strand is joined via NHEJ to a DNA terminus the loss of a functional MMR machinery, such as is from the ...
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Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
... LACZ gene on a centromere-containing plasmid such that no functional @-galactosidase was produced. This construction also includes another promoterless copy of LACZ, containi[r] ...
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Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51.
... Synthetic sensitivity of haploid 5-932 mutants to DNA damage: Our observations show that dominant-nega- tive alleles of RAD52 similarly suppressed the W and MMS sensitivi[r] ...
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A Test of the Double-Strand Break Repair Model for Meiotic Recombination in Saccharomyces cerevisiae
... The DSBR model, as modified to retain fully the 3‘- ended strands (absent mismatch correction), predicts that hDNA will be present on both DNA molecules that emerge from a[r] ...
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Tying the loose ends together in DNA double strand break repair with 53BP1
... In response to IR, the histone variant H2AX is rapidly phosphorylated by PIK kinases and relocalizes to sites of DNA damage [22,23]. The accumulation of phosphor- ylated histone H2AX ( γ -H2AX) at and near sites of DSBs ...
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A Role for REV3 in Mutagenesis During Double-Strand Break Repair in Saccharomyces cerevisiae
... Spontaneous reversion of point mutations showed a greater REV3 dependence than reversion of frameshift mutations: We investigated the role of REV3 in sponta- neous reversio[r] ...
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Double-Strand Break Repair in Tandem Repeats During Bacteriophage T4 Infection
... Recombinational repair of double-strand breaks in tandemly repeated sequences often results in the loss of one or more copies of the ...for repair has been proposed to account for this ...
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An Allele of RFA1 Suppresses RAD52-Dependent Double-Strand Break Repair in Saccharomyces cerevisiae
... Haber 1992). As the degradation proceeds past the flanking restriction enzyme sites, cleavage is blocked and results in a longer single-stranded intermediate that is easily detected with a strand-specific probe ...
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DNA double strand break repair enzymes function at multiple steps in retroviral infection
... We analyzed 216 3' junctions between HIV-1 provirus and the host DNA in a control cell line, MRC5SV, and found one abnormal junction with a single nucleotide insertion, and seven junctions with deletions in viral DNA ...
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Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms
... can break asymmetrically during mitosis, when pulled toward opposite spindle poles, in which case one cell gets a chromosome with a palindrome, whereas the other cell gets a chromosome with a ...
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Fidelity of Mitotic Double-Strand-Break Repair in Saccharomyces cerevisiae: A Role for SAE2/COM1
... the break invades the homologous se- quence on the other side of the ...displaced strand (shown) or the recently synthesized strand (not shown; see ...the break to participate in the re- ...
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The Roles of REV3 and RAD57 in Double-Strand-Break-Repair-Induced Mutagenesis of Saccharomyces cerevisiae
... residual repair, indicating (⬎97%) of the events in this substrate are due to cocon- that RAD57 and RAD55 play an important role in version rather than to BRIM ...
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SUMO modification of the neuroprotective protein TDP1 facilitates chromosomal single-strand break repair
... in repair capacity observed between TDP1 and TDP1 K111R was, at least in part, due to a role for TDP1 SUMOylation in repairing transcription-associated SSBs, then inhibiting transcription should ablate or reduce ...
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