Strand exchange
Yeast spt6-140 Mutation, Affecting Chromatin and Transcription, Preferentially Increases Recombination in Which Rad51p-Mediated Strand Exchange Is Dispensable
... synthesis-dependent strand ble-strand breaks (DSBs) in yeast (see Pa ˆques and annealing (SDSA) as proposed for Drosophila and Usti- Haber ...requires strand exchange and but also abolish ...
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DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.
... 9 DNA Strand Exchange and Selective DNA Annealing Promoted by the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein ZENTA TSUCHIHASHIt AND PATRICK 0.. BROWN* Howard Hughes Medical[r] ...
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A sister-strand exchange mechanism for recA-independent deletion of repeated DNA sequences in Escherichia coli.
... Although dimerization may be indicative of a recom- binational mechanism for deletions, these dimer prod- ucts are not characteristic of a reciprocal unequal crossing-over[r] ...
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The effect of attachment site mutations on strand exchange in bacteriophage lambda site-specific recombination.
... Segregation pattern of overlap region mutations during integrative recombination: The segregation patterns of attP-2A and attP-2G core mutations into attL or attR were mea[r] ...
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Remodeling of the Rad51 DNA Strand-Exchange Protein by the Srs2 Helicase
... Furthermore, we found that the translocase activity of Srs2 , rather than the Rad51 -binding domain, was critical for the in vivo disruption of Rad51 fi laments.. Srs2 removes Rad51 from [r] ...
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DNA strand exchange catalyzed by proteins from vaccinia virus-infected cells.
... * 204 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Vaccinia virus infection induces expression of a protein which can catalyze joint molecule formation between a sing[r] ...
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Resolution of poxvirus telomeres: processing of vaccinia virus concatemer junctions by conservative strand exchange.
... This study demonstrates that orientation of each sequence with respect to the other as well as to the axis of symmetry is critical for resolution, the processing of plasmids containing h[r] ...
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A MORE CRITICAL TEST OF HYPOTHESES OF CROSSING OVER WHICH INVOLVE SISTER-STRAND EXCHANGE
... Our conclusion-that the proposed hypothesis of somatic exchange is not sup- portable-can be further substantiated by comparing the twin-spot frequency in free X flies with the fr[r] ...
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The N-terminal domain of Escherichia coli RecA have multiple functions in promoting homologous recombination
... The RecA-ssDNA crystal structures also indicate that Arg243 and Lys245 might constitute a binding site for the donor dsDNA during the strand exchange reaction. Arg243 and Lys245 are ~25 Å away from the ...
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Homologous recombination: from model organisms to human disease
... formation of the Rad51 nucleoprotein filament [10]. RP-A is a much more tenacious single-stranded-DNA-binding protein than Rad51, however. Because of this property, RP-A can cause inhibition of DNA-strand ...
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Replication Protein A Is Required for Meiotic Recombination in Saccharomyces cerevisiae
... In Saccharomyces cerevisiae, meiotic recombination is initiated by transient DNA double-stranded breaks (DSBs). These DSBs undergo a 5⬘ → 3⬘ resection to produce 3⬘ single-stranded DNA ends that serve to channel DSBs ...
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Rad51 Protein from the Thermotolerant Yeast Pichia angusta as a Typical but Thermodependent Member of the Rad51 Family
... promoted DNA strand exchange equally well in the tempera- ture range between 32 and 42°C, but no products were de- tected at temperatures above 47°C (Fig. 7B and C). About 50% of linear DNA was converted ...
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Characterisation of a Holliday junction resolving enzyme from Schizosaccharomyces pombe
... YDC2 cuts a branch-migrating four-way DNA junction. We next wanted to determine whether the YDC2 protein pos- sessed endonuclease activity. The cellular resolving enzymes RuvC and CCE1 display sequence-specific ...
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Genetic evidence for preferential strand transfer during meiotic recombination in yeast.
... We present genetic evidence that the nontranscribed strand is donated more frequently than the transcribed strand in spores that have an unrepaired mismatch at the [r] ...
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Online Full Text
... layer strand and the diameter of the inner core and the differential contraction between the core and the wires, the contact mode may shift to that among the wires in the layer ...wired strand with a ...
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Program of bacteriophage gh-1 DNA transcription in infected Pseudomonas putida.
... Strand specificity of gh-l RNA transcription in vitroa RNA polymerase Molar ratio Hybridizaof enzyme tion to H to gh-i strand DNA cpm Hybridization to L strand cpm beled RNA synthesized [r] ...
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Heißig, Philipp Michael (2017): Oligonucleotides for enhancing intracellular delivery and monitoring intracellular stability of synthetic RNAi nanoagents. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... increases and the carrier becomes increasingly cationic and membrane-destabilizing. The endosome bursts and releases its cargo to the cytosol (proton sponge effect) [22, 23]. Membrane destabilizing peptides and membrane ...
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An assessment of the strength of knots and splices used as eye terminations in a sailing environment
... When considering methods of making eye termina- tions for a sailing environment, it is very unlikely that an improvement of a few percent in the breaking load will be sufficient to encourage the adoption of different ...
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Probability of Genetic Diagnosis
... The main study in this paper is to calculate the probability of disease in patients with disease genes. At the time of gene sequence analysis, the distance between the DNA strand and the template strand was ...
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Characterisation of unusual DNA glycosylases from Trichomonas vaginalis
... single strand breaks (SSB) with unique 3’-phosphoglycolate modified ends (Henner et ...double strand breaks through oxidation of base and sugar residues closely located on opposite strands of the double ...
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