Subgenomic mRNA synthesis
Host-dependent evolution of the Sindbis virus promoter for subgenomic mRNA synthesis.
... of subgenomic to genomic RNA synthesis in C7-10 cells, since the posttranscriptional differences described above should fa- vor the relative increase in subgenomic mRNA ...of subgenomic ...
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Characterization of an Equine Arteritis Virus Replicase Mutant Defective in Subgenomic mRNA Synthesis
... in subgenomic mRNA ...produces subgenomic positive and negative strands, albeit at a very low ...Both subgenomic positive-strand synthesis and negative-strand synthesis are ...
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The Stability of the Duplex between Sense and Antisense Transcription-Regulating Sequences Is a Crucial Factor in Arterivirus Subgenomic mRNA Synthesis
... 2002 Subgenomic mRNAs of nidoviruses (arteriviruses and coronaviruses) are composed of a common leader sequence and a “body” part of variable size, which are derived from the 5 ⴕ - and 3 ⴕ -proximal part of the ...
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The Predicted Metal-Binding Region of the Arterivirus Helicase Protein Is Involved in Subgenomic mRNA Synthesis, Genome Replication, and Virion Biogenesis
... and subgenomic mRNA transcription, are generated by extensive proteolytic ...processing. Subgenomic mRNA transcription involves an unusual discontinuous step and generates the mRNAs for ...
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DNA-Directed Expression of Functional Flock House Virus RNA1 Derivatives in Saccharomyces cerevisiae, Heterologous Gene Expression, and Selective Effects on Subgenomic mRNA Synthesis
... harness subgenomic mRNA synthesis for re- porter gene ...RNA synthesis, because reporter gene expression remains dependent on FHV RNA replication and subgenomic mRNA ...
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Arterivirus Subgenomic mRNA Synthesis and Virion Biogenesis Depend on the Multifunctional nsp1 Autoprotease
... additional, subgenomic mRNAs to regulate the expres- sion of part of their ...RNA synthesis to produce a nested set of 5 ⴕ- and 3 ⴕ-coterminal subgenomic mRNAs, which serve to express the viral ...
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Role of Nucleotides Immediately Flanking the Transcription-Regulating Sequence Core in Coronavirus Subgenomic mRNA Synthesis
... the synthesis of alternative sgmR- NAs (34, 44), suggesting that an extended complementarity between TRS-L and cTRS-B within these sequences was de- cisive for a junction event to ...sgmRNA synthesis were ...
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Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs.
... six subgenomic mRNAs is produced in equine arteritis virus (EAV)-infected ...the subgenomic EAV mRNAs, could be prepared from lysates of infected ...the mRNA 2 leader-body ...of mRNA 2, ...
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Downstream Sequences Influence the Choice between a Naturally Occurring Noncanonical and Closely Positioned Upstream Canonical Heptameric Fusion Motif during Bovine Coronavirus Subgenomic mRNA Synthesis
... and synthesis of subgenomic mRNA ...and synthesis of sgmRNA at 1, 24, and 48 h postransfection (hpt) and in cells infected with passage 1 virus (Virus ...from mRNA generated from ...and ...
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Analysis of Sindbis virus promoter recognition in vivo, using novel vectors with two subgenomic mRNA promoters.
... The four test promoters were the 19/5 promoter, which is the minimal sequence that directs detectable subgenomic mRNA synthesis (9); a 98/14 promoter, which probably has full promoter fu[r] ...
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Subgenomic mRNA of Aura alphavirus is packaged into virions.
... The yields of Aura virus particles are comparable to those produced follow- ing Sindbis virus infection, and it seems unlikely that the presence of Aura virus 26S RNA in virions results [r] ...
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Evolution of the Sindbis virus subgenomic mRNA promoter in cultured cells.
... Transcription of the subgenomic mRNA of alphaviruses initiates at an internal site, called the promoter, which is highly conserved. To determine the functional significance of this conservation, we used an ...
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Subgenomic mRNA in OK10 Defective Leukemia Virus-Transformed Cells
... Analysis of cells transformed by defective leukemia virus OK10: produc- tion of non-infectious particles and synthesis of Pr 76w and an additional 200,000 daltons protein. Labelling deox[r] ...
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IBV : potential as a vaccine vector and identification of a novel subgenomic mRNA
... negative-strand synthesis, in which part of the mRNA TRS is derived from the ...IR mRNA in the work described in Chapter 6 it was clear that one of the main reasons for the previous lack of ...
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Torovirus Non-Discontinuous Transcription: Mutational Analysis of a Subgenomic mRNA Promoter
... Of most concern, the ratio of sg DI to genomic DI RNA differs significantly from that seen for the standard virus and is almost reversed compared to the ratio of mRNA 5 to viral genome. Similar observations were ...
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A system for study of coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion.
... The in vitro- synthesized PR6 RNA (lane 1), gel-purified PR6 genomic DI RNA from PR6 replicating cells (lane 3), gel-purified subgenomic DI RNA from PR6 replicating cells (lane 4), and i[r] ...
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Transcription of Subgenomic mRNA of Hepatitis Delta Virus Requires a Modified Hepatitis Delta Antigen That Is Distinct from Antigenomic RNA Synthesis
... and subgenomic mRNA transcription is still ...HDV mRNA transcrip- ...RNA synthesis but are required for genomic RNA ...the synthesis of HDV antigenomic RNA are different from those for ...
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Size analysis and relationship of murine leukemia virus-specific mRNA's: evidence for transposition of sequences during synthesis and processing of subgenomic mRNA.
... This 38S RNA ported here, we have used specific cDNA probes codes for three classes of viral structural pro- to study the intracellular virus-specific mRNA's teins: the internal structur[r] ...
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Identification of a Noncanonically Transcribed Subgenomic mRNA of Infectious Bronchitis Virus and Other Gammacoronaviruses
... Coronavirus subgenomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving transcrip- tion regulatory sequences (TRSs) located in the 5= leader sequence (TRS-L) and ...
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Sequence Requirements for Sindbis Virus Subgenomic mRNA Promoter Function in Cultured Cells
... minimal subgenomic mRNA promoter (spanning positions ⴚ 19 to ⴙ 5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active ⴚ 98 to ⴙ 14 ...
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