subgenomic RNA
trans Regulation of Cap-Independent Translation by a Viral Subgenomic RNA
... multi- RNA viruses has been observed primarily in vitro (30, 31, 46). The RNA elements that confer translational competitiveness of viral subgenomic RNAs have been mapped to both the 5 ⬘ (17, 31, 40) ...
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Promoter for Sindbis virus RNA-dependent subgenomic RNA transcription.
... This was accomplished by inserting an AvaIl fragment from JN22 junction sequences -20 to +2 from position 1353 corresponding to -6 from the subgenomic RNA start site to position 1641 int[r] ...
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Aura alphavirus subgenomic RNA is packaged into virions of two sizes.
... The subgenomic RNA is transcribed from minus- strand copies of alphavirus genomic RNA during the process of infection and serves as mRNA for the three structural pro- teins ...virus subgenomic ...
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A Positive-Strand RNA Virus with Three Very Different Subgenomic RNA Promoters
... Numerous RNA viruses generate subgenomic mRNAs (sgRNAs) for expression of their 3 ⴕ -proximal ...BYDV RNA replication in oat ...of RNA sequences and computer- predicted secondary structures ...
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An RNA Pseudoknot Is Required for Production of Yellow Fever Virus Subgenomic RNA by the Host Nuclease XRN1
... small subgenomic RNA that is colinear with the distal part of the viral 3 ⴕ -untranslated region ...small subgenomic flavivirus RNA (sfRNA) results from the incomplete degradation of the viral ...
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Internal Initiation Is Responsible for Synthesis of Wuhan Nodavirus Subgenomic RNA
... Defining the boundaries of the WhNV subgenomic pro- moter. As noted above, WhNV sgRNA synthesis does not fit the two major steps of the premature termination model, which includes (i) generation of a ...
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Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.
... By preparing mutant RNAs with in-frame deletions in the P1 region of the genome, Kaplan and Racaniello 14 showed that most of the sequence that encodes the capsid proteins was not requir[r] ...
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Mechanism of coronavirus transcription: duration of primary transcription initiation activity and effects of subgenomic RNA transcription on RNA replication.
... This model is consistent with most previously described observations; subgenomic DI RNA synthesis does not occur in subgenomic DI RNA-transfected, MHV-infected cells 20, subgenomic RI RN[r] ...
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Generation of Subgenomic RNA Directed by a Satellite RNA Associated with Bamboo Mosaic Potexvirus: Analyses of Potexvirus Subgenomic RNA Promoter
... Satellite RNA of bamboo mosaic potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic potexvirus (BaMV) for replication and ...
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DNA-Directed Expression of Functional Flock House Virus RNA1 Derivatives in Saccharomyces cerevisiae, Heterologous Gene Expression, and Selective Effects on Subgenomic mRNA Synthesis
... for subgenomic mRNA synthesis from independently generated subgenomic negative strands have been discussed for coronaviruses (35) and for red clover necrotic mottle virus ...positive-strand ...
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A Six-Nucleotide Segment within the 3′ Untranslated Region of Hibiscus Chlorotic Ringspot Virus Plays an Essential Role in Translational Enhancement
... RNA plant viruses use various translational regulatory mechanisms to control their gene expression. Translational enhancement of viral mRNAs that leads to higher levels of protein synthesis from specific genes may ...
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Interactions between Rubella Virus Capsid and Host Protein p32 Are Important for Virus Replication
... of subgenomic RNA were significantly lower in cells infected with the 5RA virus ...the RNA replication-specific roles of capsid (9, 52) are dependent upon interactions with ...RV RNA better ...
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A Subgenomic Segment of Theiler's Murine Encephalomyelitis Virus RNA Causes Demyelination
... TMEV subgenomic RNA enters the nucleus and is ...of subgenomic segments of virus genes (with the presence of viral RNA within the nu- cleus) has been of value in clarifying the function of ...
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Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation
... LIYV RNA 1 progeny genomic and subgenomic RNAs could be detected in protoplasts as early as 12 h postinoculation ...LIYV RNA 1 open reading frame 2 (ORF 2) subgenomic RNA was the most ...
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Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... Generation of synthetic RNA templates. Sapovirus clone pJG-Sap01 (GenBank accession number AY694184), which was characterized by phylogenetic analysis based on the complete sequence of its capsid gene as belonging ...
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Identification of a Conserved RNA Replication Element (cre) within the 3Dpol-Coding Sequence of Hepatoviruses
... replication-competent, subgenomic RNA replicon, HAVLuc, in which an in-frame fusion of the firefly luciferase coding sequence replaces most of the P1, capsid coding region of the genome of a rapidly ...
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Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination.
... Our strategy for repairing the deletion in Alb4 was to transfect cells with Alb4 genome RNA and wild-type subgenomic RNA7, the smallest MHV subgenomic RNA, which is a monocistronic mRNA [r] ...
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Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase
... disrupted, RNA replication was greatly reduced (18), whereas disruption of the putative minus-strand stem-loop structure was not correlated with effects on viral replication ...plus-strand RNA is involved ...
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JBC-2005-Huang-36417-28.pdf
... strand RNA maps to the polypyrimidine tract, an element known to be essential for genome replication and ...gle-stranded RNA containing stretches of uridine or ...Moreover, RNA immunoprecipitation ...
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jvirol00090-0376.pdf
... These data suggest that an N binding site is present within the leader RNA sequences at a site at least 65 nucleotides from the 5' end of genomic RNA and all six subgenomic mRNAs.. The l[r] ...
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