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subgingival plaque

Comparison of Real Time PCR and Culture for Detection of Porphyromonas gingivalis in Subgingival Plaque Samples

Comparison of Real Time PCR and Culture for Detection of Porphyromonas gingivalis in Subgingival Plaque Samples

... Periodontitis lesions are associated with a complex subgin- gival microflora which consists mainly of gram-negative bacte- rial species (37), of which the dark-pigmented organism Por- phyromonas gingivalis is considered ...

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Detection and Quantification of Oral Treponemes in Subgingival Plaque by Real Time PCR

Detection and Quantification of Oral Treponemes in Subgingival Plaque by Real Time PCR

... to subgingival plaque sampling, each tooth was isolated with cotton rolls and air dried, and then the pocket depth was measured by ...Supragingival plaque was first removed with sterile cotton, and ...

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High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia

High throughput DNA sequencing to detect differences in the subgingival plaque microbiome in elderly subjects with and without dementia

... These sequences were analyzed using bioinformatics tools available in the publicly accessable software package Quantitative Insights into Microbial Ecology (QIIME) [21], and sequence comparisons were made among parti- ...

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Molecular Identification of Dialister pneumosintes in Subgingival Plaque of Humans

Molecular Identification of Dialister pneumosintes in Subgingival Plaque of Humans

... the subgingival microbiota may have missed the ...of subgingival iso- lates. This study showed four subgingival specimens containing more than 5% cultivable ...the subgingival plaque of ...

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Negative Correlation of Distributions of Streptococcus cristatus and Porphyromonas gingivalis in Subgingival Plaque

Negative Correlation of Distributions of Streptococcus cristatus and Porphyromonas gingivalis in Subgingival Plaque

... dental plaque samples were determined and compared with the numbers of ...the plaque sam- ples where the number of ...dental plaque are due to repression of FimA production induced by the presence of ...

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Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients

Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients

... As expected, none of the A.actinomycetemcomitans 16 s rDNA negative samples were positive for cdtB . This result confirmed that A.actinomycetemcomitans was the exclusive member in the oral microbial flora identified to ...

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Microbiological Analysis of Periodontal Pocket (Subgingival Plaque) and Coronary Atheromatous Plaque in Atherosclerotic Patients

Microbiological Analysis of Periodontal Pocket (Subgingival Plaque) and Coronary Atheromatous Plaque in Atherosclerotic Patients

... The study was performed on twenty five individuals with atherosclerosis. Atheromatous Plaques were collected from the patients during endarterectomy and the subgingival plaque was collected from the same ...

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Biofilm structure and cell vitality in a laboratory model of subgingival plaque

Biofilm structure and cell vitality in a laboratory model of subgingival plaque

... Participants in the study were assigned to one of five test groups based on the search tool they were asked to use while completing a “search process interview”: EDS, Summon, GS, the “co[r] ...

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Pseudomonas aeruginosa and Periodontal Pathogens in the Oral Cavity and Lungs of Cystic Fibrosis Patients: a Case Control Study

Pseudomonas aeruginosa and Periodontal Pathogens in the Oral Cavity and Lungs of Cystic Fibrosis Patients: a Case Control Study

... the subgingival plaque and sputum ...in subgingival plaque is not a transitory event and the oral cavity may be a reservoir for pathogens ...

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Assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study

Assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study

... val plaque pathogens were useful indicators of periodontal ...in subgingival plaque had the potential to help identify sites at significant risk for progression of periodontitis ...in ...

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Culture Independent Identification of Periodontitis Associated Porphyromonas and Tannerella Populations by Targeted Molecular Analysis

Culture Independent Identification of Periodontitis Associated Porphyromonas and Tannerella Populations by Targeted Molecular Analysis

... Periodontitis is the commonest bacterial disease of humans and is the major cause of adult tooth loss. About half of the oral microflora is unculturable; and 16S rRNA PCR, cloning, and sequencing techniques have ...

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Application of monoclonal antibodies to the detection of black pigmented Bacteroides spp  in subgingival plaques by immunoslot blot assay

Application of monoclonal antibodies to the detection of black pigmented Bacteroides spp in subgingival plaques by immunoslot blot assay

... Subgingival plaque samples from adult periodontal patients were examined by immunoslot blot assay with monoclonal antibodies that specifically recognize Bacteroides gingivalis, Bacteroid[r] ...

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Analysis of Orange Complex Bacteria in the microbiome of whole Saliva in Periodontal Health and Periodontitis Individuals using Next Generation Sequencing Technology

Analysis of Orange Complex Bacteria in the microbiome of whole Saliva in Periodontal Health and Periodontitis Individuals using Next Generation Sequencing Technology

... in subgingival plaque by studying 13,261 plaque samples from 185 subjects using whole genomic DNA probes and checkerboard DNA-DNA ...of subgingival plaque taken from subjects with ...

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Dynamic Changes in the Subgingival Microbiome and Their Potential for Diagnosis and Prognosis of Periodontitis

Dynamic Changes in the Subgingival Microbiome and Their Potential for Diagnosis and Prognosis of Periodontitis

... hygiene, subgingival plaques were col- lected from two affected tooth sites per subject on ...supragingival plaque was re- moved. The subgingival plaque samples were then collected using ...

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An Evaluation of Microbial Profile in Halitosis with Tongue Coating Using PCR (Polymerase Chain Reaction)- A Clinical and Microbiological Study

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... Both the tanita & organoleptic scores & tongue microbial profile showed a weak positive correlation with tongue coating. The Fn count in tongue coat was found be lower than in subgingival plaque ...

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Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples

Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples

... The probes were used to detect the organism directly from nucleic acid extracts of subgingival plaque samples, and the results were compared with results of detection by culture methods.[r] ...

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Association of Three Bacterial Species and Periodontal Status in Chinese Adults: an Epidemiological Approach

Association of Three Bacterial Species and Periodontal Status in Chinese Adults: an Epidemiological Approach

... in subgingival plaque collected from 468 subjects with chronic periodontitis in a group of Chinese adults by using a PCR method and to determine the degree of association between the target bacteria and ...

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Response of Subgingival Bacteria to Smoking Cessation

Response of Subgingival Bacteria to Smoking Cessation

... Subject selection and study design. Ethical approval for this study was ob- tained from the Newcastle Research Ethics Committee and the Office of Re- sponsible Research Practices at The Ohio State University. The study ...

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Oral microbiota in youth with perinatally acquired HIV infection

Oral microbiota in youth with perinatally acquired HIV infection

... and subgingival plaque samples has shown overall simi- lar microbial composition between HIV-positive and HIV-negative adults, some differences are notable [8– ...

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Impact of aging on TREM 1 responses in the periodontium: a cross sectional study in an elderly population

Impact of aging on TREM 1 responses in the periodontium: a cross sectional study in an elderly population

... in subgingival plaque samples harvested from patients with gingivitis and CP, than in those from healthy participants (p < ...the subgingival levels of the other four periodontal pathogens and ...

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