Substrate recognition
Cellulose-specific Type B carbohydrate binding modules: understanding oligomeric and non-crystalline substrate recognition mechanisms
... improvement. Substrate composition and crystallinity, as well as efficient substrate recognition by enzymes, are a few of the important factors that determine the yield and rate of enzymatic ...
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Structure and substrate recognition of the Bottromycin maturation enzyme BotP
... Abstract: The bottromycins are a family of highly modified peptide natural products displaying potent antimicrobial activity against Gram-positive bacteria including methicillin-resistant Staphyloccoccus aureus. ...
15
The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases
... inhibits substrate binding and is a regulatory mechanism to maintain proper epithelial Na+ transport ...for substrate recognition, but also for the regulation of ...Pellino substrate ...
103
Flavivirus enzyme-substrate interactions studied with chimeric proteinases: identification of an intragenic locus important for substrate recognition.
... Although the chimeric proteinases did not allow direct mapping of regions involved in substrate recognition 21, the results with them do suggest that residues 144 to 180 are important fo[r] ...
10
Atlas on substrate recognition subunits of CRL2 E3 ligases
... The later research also indicates that LRR-1 acts as a nuclear substrate-recognition subunit of a CRL2 complex, which ensures DNA replication integrity [59]. Loss of LRR-1 function induces re-replication of ...
10
Molecular Mechanisms of Viral and Host Cell Substrate Recognition by Hepatitis C Virus NS3/4A Protease
... NS3/4A substrate sequences are recognized in a conserved three-dimensional shape, defining a consensus van der Waals volume, or substrate envelope ...of substrate recognition regulates ...
11
Triglycine-Based Approach for Identifying the Substrate Recognition Site of an Enzyme
... X-ray crystal structure confirmed that triglycine binds at the PaProK substrate recognition site 144. in two different conformations[r] ...
9
DNA substrate recognition and processing by the full-length human UPF1 helicase
... independent, substrate recognition by helicases can be structure depen- ...mRNA recognition, ss- DNA length and sequence are the primary factors influ- encing hUPF1 substrate ...
13
The structure of Toho1 β lactamase in complex with penicillin reveals the role of Tyr105 in substrate recognition
... The role of the conserved residue Tyr105 in class A b -lactamases has been the subject of investigation using both structural studies and saturation mutagenesis. Both have shown that while it does not need to be strictly ...
9
The C terminal GGAP motif of Hsp70 mediates substrate recognition and stress response in yeast
... for diasporic communities makes it more than whimsy (Burdsey et al., 2013) and its analysis requiring a shift from the ‘toy department of human affairs’ (Edwards, 1979: 116) to a clear recognition that sport ...
12
Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein.
... In addition, the enzyme may recognize the polarity of the two strands at the cut site, or it may recognize another sequence element within the stem of the hairpin that is required for co[r] ...
9
Structural Insights into Thermotoga maritima FtsH Periplasmic Domain on Substrate Recognition
... a substrate is bound, the equilibrium would be shifted in favor of PD hexamerization (Figure ...the substrate and enclose it inside the central channels of the hexameric PD, the TM domain, and the peptidase ...
15
Determinants of substrate recognition by poliovirus 2A proteinase.
... The mechanism which limits proteolytic processing to specific sites has not been fully characterized, but by analogy with other viral proteinases 6, 7, 23, it is likely that substrate re[r] ...
9
Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD
... tetrapeptide substrate analogue succinyl-Ala-Leu-Pro-Phe-4-nitroanilide (suc-ALPF-pNA) or variants thereof ...ideal substrate mimics, because they bind to Escheri- chia coli SlyD with much lower affinity ...
25
ADAMTS13 substrate recognition of von Willebrand factor A2 domain
... to start each reaction. For analyses of the intact A2 domain, reactions were performed in the presence and absence of 1 M urea. At different time points, 65-l sub-samples were removed and stopped with EDTA. 15 l were ...
9
Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase
... the substrate-binding site. A peptide-flip does not accompany substrate binding since apo-LmMK molecules retain the strained ...for substrate so proving an important role in substrate binding ...
16
Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
... Sequence alignment shows that KsdD3 shares at most 46% sequence identity with R. erythropolis SQ1-KsdD1, which gives us only a traditional Rossmann fold (nucleo- tide-binding fold), a FAD binding domain and a catalytic ...
13
Degradation of Saccharomyces cerevisiae Transcription Factor Gcn4 Requires a C-Terminal Nuclear Localization Signal in the Cyclin Pcl5
... Cyclin-dependent kinases (CDKs) are protein kinases (7) that are activated by binding of an auxiliary subunit, the cyclin (7, 20). Cyclins play an important role in targeting the kinase to specific substrates (15, 49, ...
15
VAMP-2 Q58 -K 59 peptide bond exhibit unique
... Q 58 /K 59 bond in VAMP-2 but exhibit unique catalytic and substrate recognition properties versus 40.. the prototype BoNT/F1.[r] ...
18
In vitro biosynthetic studies of bottromycin expand the enzymatic capabilities of the YcaO superfamily
... We next investigated if the BmbC follower peptide played a role in substrate recognition. Stop codons (denoted as asterisks) were in- troduced to produce C-terminal truncations of BmbC at intervals of five ...
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