substrate specificity
Bacteriophage display of peptides and its use in determining proteolytic substrate specificity
... characterizing substrate specificity of proteases, the technique can be utilized for various other purposes like identifying specific antibodies, interaction with specific ligands and many ...
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Functional identification of nucleotides conferring substrate specificity to retroviral integrase reactions.
... In summary, each of the retroviral INs exhibited unique substrate specificity traits. The comparative study presented here indicates that the nucleotides in the LTR termini present some structural ...
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Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease
... tended substrate interactions, it is unusual that HIV PR is able to catalyze proteolysis of the tetrapeptide ACC substrates, in which only the coumarin binds on the prime ...the specificity constants for ...
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Substrate specificity of Cryptococcus albidus and Eupenicillium erubescens α-L-rhamnosidases
... The substrate specificity of Cryptococ- cus albidus and Eupenicillium erubescens α-L- rhamnosidases has been ...narrow specificity for glycon: ...
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Evolutionary Selection on Barrier Activity: Bar1 Is an Aspartyl Protease with Novel Substrate Specificity
... unusual substrate specificity of Bar1 is a direct result of coevolution with Ste2, the receptor for ␣ pheromone, for recognition of the same peptide ...novel specificity of Bar1 reveals the complex ...
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Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina
... Background: The understanding of enzymatic polysaccharide degradation has progressed intensely in the past few years with the identification of a new class of fungal-secreted enzymes, the lytic polysaccharide ...
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Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase
... its substrate specifi- city is affected by protein ...The substrate specificity of MT is significantly af- fected by the protein context within which it is ...
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Amino Acid Residues Contributing to the Substrate Specificity of the Influenza A Virus Neuraminidase
... NA substrate specificity in viral replication among different animal ...the substrate speci- ficities of NA for linkages between N-acetyl sialic acid and galactose (NeuAc ␣ 2-3Gal and NeuAc ␣ 2-6Gal) ...
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Furaldehyde substrate specificity and kinetics of Saccharomyces cerevisiae alcohol dehydrogenase 1 variants
... The results presented here unravel the impact of single mutations on the substrate specificity of a key S. cerevisiae metabolic enzyme and identify tyrosine 295 as the key amino acid to mutate for getting ...
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Evolution of substrate specificity in a recipient's enzyme following horizontal gene transfer
... The core view of enzyme evolution is that gene duplication of multi-specific enzymes, followed by narrowing of substrate specificity, is the primary mechanism by which novel enzyme families have evolved ...
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MECHANISTIC AND SUBSTRATE SPECIFICITY STUDIES ON Burkholderia mallei QUORUM SENSING SIGNAL SYNTHESIS ENZYME
... and substrate specificity details for these enzymes are not well ...C8ACP substrate, in-line with tight signal specificity observed in ...
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Amino acids determining enzyme-substrate specificity in prokaryotic and eukaryotic protein kinases
... We now turn our attention to positions 191 and 196. The residues are not in contact with the inhibitor in PKA (Fig. 2d), so it seems unlikely that Val-191 or Trp-196 plays a direct role in recognizing the ...
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Rapid Triacylglycerol Turnover in Chlamydomonas reinhardtii Requires a Lipase with Broad Substrate Specificity
... We first examined the expression of CrLIP1 in yeast. As ex- pected for a lipase, the majority of recombinant CrLIP1 was asso- ciated with the membrane-enriched fraction that also included lipid droplets and TAG (Fig. 1A ...
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An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes
... Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair, transcription and replication. In this paper, we describe a novel assay that ...
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Changing the substrate specificity of arogenate dehydratases (ADTs) from Arabidopsis thaliana.
... Evidence in the literature suggests that enzymatic function can be converted between two similar proteins by introducing only a few amino acid substitutions to the protein sequence (Jäckel et al. 2008). This raises the ...
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Restricted Substrate Specificity for the Geranylgeranyltransferase-I Enzyme in Cryptococcus neoformans: Implications for Virulence
... The results of this study demonstrate that the C. neoformans Ggtase-I plays a conserved role in Cdc42 and Rho10 protein local- ization but that it is not likely to be the major CaaX prenyltrans- ferase in this species. ...
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The substrate specificity of hormone sensitive lipase from adipose tissue of the Antarctic fish Trematomus newnesi
... In mammals, it is clear that the release of fatty acids from adipose tissue is not random. For example, in rabbit adipose tissue, mobilization of fatty acids into the plasma was not proportional to their abundance but ...
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Probing the substrate specificity of hepatitis C virus NS3 serine protease by using synthetic peptides.
... in-depth substrate specificity ...NS5A/5B substrate peptide by alanine-scanning ...peptide substrate also revealed that the P1 and P3 residues of NS4A/4B were most important ...
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Evolution of substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases
... control substrate specificity, we compared homology models for 43 proteins that spanned the AA10 family (Figure 5) across the clade I and clade II se- quences shown in Figure ...the substrate binding ...
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Insights into Substrate Specificity of NlpC/P60 Cell Wall Hydrolases Containing Bacterial SH3 Domains
... Evolution of SH3b domains and SH3b-NlpC/P60 fusion pro- teins. Although the SH3b domains studied here are extremely diverse in sequence ( ⬍ 10% pairwise sequence identity), they all share a core consisting of seven ...
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