Sulfolobus islandicus
The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial molecules
... Results: A novel (CAMP)-like peptide (VLL-28) was identified in the primary structure of a transcription factor, Stf76, encoded by pSSVx, a hybrid plasmid–virus from the archaeon Sulfolobus islandicus. ...
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Formation of a Viral Replication Focus in Sulfolobus Cells Infected by the Rudivirus Sulfolobus islandicus Rod-Shaped Virus 2
... DNA polymerase Dpo1 is primarily responsible for SIRV2 genome replication. The main role of Dpo1 in SIRV2 genome replication was further investigated in the individual knockout (KO) mutants of Dpo2, Dpo3, and Dpo4 and ...
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Virus Induced Dormancy in the Archaeon Sulfolobus islandicus
... concentrated S. islandicus strain Y08.82.36 (see Table S1), a highly suscep- tible Sulfolobus islandicus host isolated from Yellowstone National Park. Cells mixed with virus dilutions were plated on ...
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Cell Structure Changes in the Hyperthermophilic Crenarchaeon Sulfolobus islandicus Lacking the S Layer
... S. islandicus S-layer gene deletion mutants (26) provide strong and direct in vivo evidence in support of a so-called stalk-and-cap model of the S-layer proposed in Sulfolobales (9, ...related Sulfolobus ...
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The Structure of the NTPase That Powers DNA Packaging into Sulfolobus Turreted Icosahedral Virus 2
... Sulfolobus turreted icosahedral virus 2 (STIV2) was isolated from the hyperthermoacidophilic archaeon Sulfolobus islandicus originating from an acidic hot spring (88.3°C, pH 3.5) and has a ...
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Unveiling Cell Surface and Type IV Secretion Proteins Responsible for Archaeal Rudivirus Entry
... in Sulfolobus solfataricus P2 ...that Sulfolobus islandicus rod-shaped virus 2 (SIRV2) enters the host cell by at- taching and moving through a pilus-like filament; however, the nature of the ...
5
Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase
... about 70% with its closest homologues, i.e. SsoPox from Sulfolobus solfataricus [3] and SisLac from Sulfolobus islandicus [33,45]. Being an enzyme from a hyperther- mophile, SacPox is however less ...
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Novel Sulfolobus Virus with an Exceptional Capsid Architecture
... SEV1 infection resulted in no significant retardation in the growth of the host cell, producing no detectable cell debris. This observation agrees with the ability of the SEV1-infected cells to form colonies. However, the ...
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Sulfolobus Turreted Icosahedral Virus c92 Protein Responsible for the Formation of Pyramid-Like Cellular Lysis Structures
... in Sulfolobus solfa- taricus demonstrated that STIV was a lytic virus and that cell lysis was preceded by the formation of unusual pyramid-like structures on the cell surface ...virus, Sulfolobus ...
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First Insights into the Entry Process of Hyperthermophilic Archaeal Viruses
... host, Sulfolobus islandicus LAL14/1 (29), represent a valuable model system to study virus-host interactions in Archaea ...SIRV2-S. islandicus interaction, focusing on the early stages of ...
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Characterisation of proteins involved in CRISPR mediated antiviral defence in Sulfolobus solfataricus
... 2006 between the system components and the eukaryotic RNA interference led a number of groups to propose that the CRISPR loci and their associated genes represent an adaptive prokaryotic[r] ...
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A Novel Type of Polyhedral Viruses Infecting Hyperthermophilic Archaea
... Virus genome. The genome of SPV1 was extracted from highly purified virions and sequenced using the Illumina platform. Assembly of the sequencing reads resulted in a single circular contig of 20,222 bp. The viral genome ...
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Cloning and functional-analysis of the tata-binding protein from Sulfolobus-shibatae
... This speculation has been validated recently by the discovery of a Pyrococcus woesei protein that is related to the eukaryotic RNA polymerase II transcription factor TFIIB (14,15) and th[r] ...
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Genetic Analysis of the Major Capsid Protein of the Archaeal Fusellovirus SSV1: Mutational Flexibility and Conformational Change
... 12. Quemin, E. R. J.; Pietila, M. K.; Oksanen, H. M.; Forterre, P.; Rijpstra, W. I. C.; Schouten, S.; Bamford, D. H.; Prangishvili, D.; Krupovic, M. Sulfolobus spindle- shaped Virus 1 contains glycosylated capsid ...
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Unification of the Globally Distributed Spindle-Shaped Viruses of the Archaea
... Over the years, a number of spindle-shaped viruses that could not be assigned to the existing taxa based on genome similarity have been isolated from phylogenetically distant archaeal lineages, including Thermococcales ...
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Structure of A197 from Sulfolobus Turreted Icosahedral Virus: a Crenarchaeal Viral Glycosyltransferase Exhibiting the GT-A Fold
... It is highly unlikely that most of the STIV proteome consists of unique protein folds serving novel functions. Rather, like the major capsid protein, the general lack of sequence similar- ity in the STIV open reading ...
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In Silico Analysis of Occurrence of Tricorn Protease and Its Homologs
... protease, Sulfolobus salfataricus, 4; Tricorn protease, Pyrobaculum aerophilum, 1; hypothetical protein, Aminicenantes bacterium 5; tricorn protease, Vibrio cholerae, 6; tricorn protease, Streptomyces coelicor, 7; ...
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Particle Assembly and Ultrastructural Features Associated with Replication of the Lytic Archaeal Virus Sulfolobus Turreted Icosahedral Virus
... Little is known about the replication cycle of archaeal viruses. We have investigated the ultrastructural changes of Sulfolobus solfataricus P2 associated with infection by Sulfolobus turreted icosahedral ...
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Responses of hyperthermophilic crenarchaea to UV irradiation
... RNA from S. solfataricus P2 was extracted at time points 30, 60, 90 and 120 minutes following UV irradiation, and puri- fied using an RNeasy Kit (Qiagen) according to the manufac- turer's directions, with the ...
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Developing novel single molecule analyses of the single stranded DNA binding protein from Sulfolobus solfataricus
... SSBs from archaea show a large variation in structure and sequence, displaying common features with both eukaryote and prokaryote SSBs, typically employing OB folds. The euryarchaeon Methanosarcina acetivorans has three ...
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