suppression subtractive hybridization
Identification of novel genes involved in gastric carcinogenesis by suppression subtractive hybridization
... performed suppression subtractive hybridization (SSH) on gastric adenocarcinoma tissue and the correspond- ing normal gastric tissue, and eight genes were found to be overexpressed in the tumor ...
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Investigation of an idiopathic lesion in redclaw crayfish Cherax quadricarinatus using suppression subtractive hybridization
... Suppression subtractive hybridization (SSH) was performed to generate a cDNA forward library between the two populations of crayfish. A total of 323 sequences were analysed from SSH libraries. These ...
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Suppression Subtractive Hybridization Technique in Wheat for the Identification of Disease Resistance Differentially Expressed Genes
... recipient. Suppression subtractive hybridization (SSH) is a powerful technique for the identification of disease specific differentially expressed genes that are expressed in a resistant or ...
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Suppression subtractive hybridization to investigate viruses in the lymphoid organ of Penaeus merguiensis and the gills of Cherax quadricarinatus
... 2.1. Introduction The rapid development of the cultured prawn industry is associated with environmental and sociological disturbances in land use, the ecology of the aquatic organisms and global trade patterns. A major ...
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Comparison of RNA expression profiles on generations of Porphyra yezoensis (Rhodophyta), based on suppression subtractive hybridization (SSH)
... the suppression subtractive hybridization (SSH) method The GO annotation showed that 58% cellular components of carpospores were located in cytoplasm and nucleus; while vegetative cells were mainly ...
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Expressed genes in the lymphoid organ of broodstock banana prawn (Penaeus merguiensis) using suppression subtractive hybridization
... Most systemic viral infections in penaeid prawns are associated with the formation of lymphoid organ spheroid (LOS) cells in the lymphoid organ (LO). The development of spheroids is suggested to be a major defence ...
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Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells
... of suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with ...
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Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization
... utilize suppression subtractive hybridization (SSH) to characterize the change in gene expression during conceptus transformation from spherical (8–9 mm) to tubular (15–40 mm) to early filamentous ...
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Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization
... In order to find new regulated transcripts, however rare, we have used a Suppression Subtractive Hybridization approach (SSH) on pig granulosa cells in primary culture treated or not with FSH. Two ...
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Differential Regulation of Proteins and a Possible Role for Manganese Superoxide Dismutase in Bioluminescence of Panellus stipticus Revealed by Suppression Subtractive Hybridization
... This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Suppression subtractive hybridization (SSH) was ...
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Enhanced Expression of Interferon-Regulated Genes in the Liver of Patients with Chronic Hepatitis C Virus Infection: Detection by Suppression-Subtractive Hybridization
... Hepatitis C virus (HCV) infection causes acute and often also chronic liver disease. Worldwide, prevalence of infection is estimated to exceed that of human immunodeficiency virus infection fourfold. Because of the lack ...
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Suppression Subtractive Hybridization as a Strategy To Identify Taxon Specific Sequences within the Mycoplasma mycoides Cluster: Design and Validation of an M capricolum subsp capricolum Specific PCR Assay
... The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia 3 Received 14 August 2007/Returned for modification 20 September 2007/Accepted 21 January 2008 The phylogenetically related Mycoplasma ...
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Suppression subtractive hybridization to investigate viruses in the lymphoid organ of Penaeus merguiensis and the gills of Cherax quadricarinatus
... 195 Genes/molecules Elongation factor-1α Elongation factor 2 Nucleosome remodelling factor e 38kD, isoformA Protein disulfide isomerase Proteasome 25 kDa subunit Proteasome 26S ATPase su[r] ...
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Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot blot hybridization
... strain subtractive cDNA libraries was blotted in duplicate onto two positively charged nylon membranes (Roche Applied Science) in a ...each subtractive cDNA library were prehybridized for 30 min at 42°C ...
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Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)
... employed. Subtractive suppression hybridization (SSH) is an effective method that can be used to maximise the identification of genes that are involved in host responses to pathogen infection and ...
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Subtractive Hybridization Techniques to Study Cellular Senescence
... expression. Subtractive cDNA hybridization is a powerful approach to identify and isolate differentially expressed genes in various ...termed suppression subtractive hybridization ...
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Subtractive hybridization-assisted screening and characterization of genes involved in the rice-Magnaporthe oryzae interaction
... Suppression subtractive hybridization (SSH) is a PCR- based approach to highlight differentially expressed genes between two populations of transcripts (Diatchenko et ...the subtractive ...
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Use of Subtractive Hybridization To Identify a Diagnostic Probe for a Cystic Fibrosis Epidemic Strain of Pseudomonas aeruginosa
... Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain ...
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Establishment of Methylation Sensitive Suppression Subtraction Hybridization Analysis and Isolation Of 5’cpg Islands Fragments in Chinese Fir Genomic DNA
... [8], methylation-sensitive single nucleotide primer extension (MS- SnuPE) Herman et al. [10], electrochemistry Kuppuswamy et al. [11] and so on. However, each method was restricted to the evaluation of DNA methylation on ...
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Differential gene expression at coral settlement and metamorphosis: a subtractive hybridization study
... suppressive subtractive hybridization (SSH) method of the PCR-Select system (Clontech) was used to produce two pools of cloned cDNA sequences corresponding to transcripts whose expression is higher in pre- ...
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