Sv40 promoter
A Recombinant Rhesus Cytomegalovirus Expressing Enhanced Green Fluorescent Protein Retains the Wild-Type Phenotype and Pathogenicity in Fetal Macaques
... To facilitate identification of rhesus cytomegalovirus (RhCMV)-infected cells, a recombinant virus express- ing enhanced green fluorescent protein (EGFP), designated RhCMV-EGFP, was constructed. An expression cassette ...
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PubMedCentral-PMC4863234.pdf
... well-known promoter for ON bipolar cell targeting is the mGluR6 ...basal SV40 promoter 8,14,15 ; however, AAV- mediated expression with the mGluR6 promoter in retinal bipolar cells is ...and ...
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Characterisation of a bagpipe homologue in Xenopus laevis
... reporter construct containing GAL4 DNA binding sites upstream of an SV40 promoter controlling firefly luciferase, in transfected ES cells. x axis Ga14 fusion transfected. [r] ...
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Role of the Membrane-Spanning Domain of Human Immunodeficiency Virus Type 1 Envelope Glycoprotein in Cell-Cell Fusion and Virus Infection
... an SV40 promoter-based ex- pression vector in the absence of other viral proteins or in the context of full-length proviral vectors, suggesting that specific amino acid residues within the HIV-1 MSD core ...
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Construction and properties of a recombinant herpes simplex virus 1 lacking both S-component origins of DNA synthesis.
... During construction of a virus in which a chimeric cx4 gene consisting of the coding domain of the gene fused to the SV40 promoter was recombined into the viral genome, a recombinant vir[r] ...
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Chromatin Structure of the Simian Virus 40 Late Promoter: a Deletional Analysis
... the SV40 promoter region, although the mechanisms leading to its formation and maintenance are not very well understood (11, 16, 17, 19, ...an SV40 recombinant reporter system (14) which has allowed ...
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PubMedCentral-PMC5599748.pdf
... inhibited SV40 promoter activity in EPC cells, which is consistent with the suppression of host protein expression being a conserved role of M among ...
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Heterologous Viral Promoters Incorporated into the Human Cytomegalovirus Genome Are Silenced during Experimental Latency
... cellular promoter but of heterologous viral promoters within the context of a herpesvirus ...the SV40 promoter to permit the facile monitoring of viral infections with fluorescence microscopy ...that ...
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A new Epstein-Barr virus transactivator, R, induces expression of a cytoplasmic early antigen.
... The region of the EBV genome containing the rightward divergent promoter and the EA-R gene pMH53 was cotransfected with BamHI-Z under the regulation of the SV40 promoter pPL17 A, HindIII[r] ...
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Characteristics of the Adeno-Associated Virus Preintegration Site in Human Chromosome 19: Open Chromatin Conformation and Transcription-Competent Environment
... FIG. 4. Transcriptional activity of DHS-S1. (A) Structure and activity of constructs containing DHS-S1 cloned upstream of the CAT gene. The whole DHS-S1 was excised as an SmaI-SmaI fragment (nt 18 to 353 of AAVS1) from ...
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Glial Cell-Specific Regulation of the JC Virus Early Promoter by Large T Antigen
... and SV40 early promoters four- to fivefold in U87MG glioma cells ...early promoter in nonglial cells, whereas the SV40 promoter was ...early promoter in other nonglial cells ...basal ...
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Use of a Murine Cytomegalovirus K181-Derived Bacterial Artificial Chromosome as a Vaccine Vector for Immunocontraception
... the SV40-derived transcrip- tion termination signal that was present twice in the BAC ...its SV40-derived transcription termination signal were removed to prevent this from ...
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Glucocorticoid regulation of murine leukemia virus transcription elements is specified by determinants within the viral enhancer region.
... In vitro CAT activity of extracts derived from HeLa cell cultures that transiently expressed transfected plasmids containing the enhancer-proficient SV40 early region promoter SV2, the e[r] ...
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Alternate translation initiation on hepatitis B virus X mRNA produces multiple polypeptides that differentially transactivate class II and III promoters.
... The results obtained from transfection of mutant pX9/79 suggested either that the transactivation of the SV40 enhancer/early promoter by X protein requires the full-length 17-kDa species[r] ...
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Role of the Innate Immune Response and Tumor Immunity Associated with Simian Virus 40 Large Tumor Antigen
... on SV40 Tag-mediated immune reactions ...in SV40 Tag-immunized ...enhanced SV40 Tag-specific CTL response rel- ative to wild-type ...recombinant SV40 Tag immunization induces a predominant Th2 ...
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Enterovirus 68 3C Protease Cleaves TRIF To Attenuate Antiviral Responses Mediated by Toll-Like Receptor 3
... we generated TRIF variants carrying residues 1 to 653, 1 to 312, 313 to 712, and 313 to 653 (Fig. 7A). In reporter assays, full-length TRIF activated the IFN- (Fig. 7E) and NF-B (Fig. 7F) promot- ers. The 1– 653 ...
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Effects of the simian virus 40 origin of replication on transcription from the human immunodeficiency virus type 1 promoter.
... intact SV40 origin, essentially equivalent to pWP0 ...basal promoter activity of the origin-containing plasmids as reported previously ...that SV40- induced replication or some closely related ...
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Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.
... A mutant similar to dlA2413 and dlA2414 has been described previously 17 and is defective for viral DNA replication and transformation of NIH 3T3 cells; the DNA binding and ATPase activi[r] ...
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Liver-specific expression of hepatitis B virus is determined by the combined action of the core gene promoter and the enhancer.
... We demonstrated that under the control of the heterologous SV40 enhancer, the promoter expresses low activity in nonliver CV-1 and HeLa cells, intermediate activity in Hep-SK cells, and [r] ...
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Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 and type 18 P105 promoters.
... Cells were transfected with pSV2CAT contains the SV40 enhancer and early promoter upstream of the CAT gene, no DNA none, a construct containing the wild-type HPV-18 LCR upstream of the C[r] ...
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