synthetic DNA
Who are the users of synthetic DNA? Using metaphors to activate microorganisms at the center of synthetic biology
... the synthetic biology lab as “ experimental partners ” with certain characteristics patterned by what they are described as being like, and of which certain expectations are there- fore ...the DNA design ...
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Direct detection of the common Mediterranean beta thalassemia gene with synthetic DNA probes An alternative approach for prenatal diagnosis
... uncloned DNA by restriction enzyme analysis. Using synthetic DNA of 19-nucleotides in length corresponding to the normal and mutant IVS-1 sequences as probes, we have developed a direct assay for ...
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From synthetic DNA to PCR product : detection of fungal infections using SERS
... thiolated DNA was purchased from ATDbio (Southampton, UK), treated with DTT then HPLC purified to remove di-thiols and other potential contaminants prior to ...moieties; DNA sequence for specific detection, ...
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The impact of processing on the biophysical properties of synthetic DNA complexes for gene delivery
... of DNA complexes has been shown to be affected by a number of factors including the degree of condensation, concentration of DNA and complexing agent, charge ratio, pH and ionic strength, size o f the ...
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Investigating and Manipulating Immune Responses to Hepatotropic Pathogens Using Synthetic DNA
... antigen or a pVax control. The injection sites were electroporated (EP) immediately following injection. We first looked at the humoral response induced by the constructs, since DNA vaccines have not always been ...
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Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides
... kb synthetic segment (gray solid arrow) to the yeast containing the landing pad ...the synthetic DNA segment into the genome via the loxP sites catalyzed by Cre recombinase, represented by purple ...
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Determination of the poliovirus RNA polymerase error frequency at eight sites in the viral genome.
... Poliovirion RNA uniformly labeled with 32P1 was hybridized to a synthetic DNA oligonucleotide that was complementary to a sequence in the viral genome that contained a single internal G [r] ...
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Addressing the shortcomings of three recent bayesian methods for detecting interspecific recombination in DNA sequence alignments
... generated synthetic DNA sequence alignments with the HKY nucleotide substitution model (Hasegawa et ...the DNA sequence alignments generated in our study, using the authors’ ...
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Inborn errors in RNA polymerase III underlie severe varicella zoster virus infections
... AT-rich DNA was reduced in PBMCs from patients, therefore con- firming previous results on POL III as an innate sensor of AT-rich DNA (26, ...that synthetic DNA derived from an AT-rich region ...
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Oligonucleotide probes for detection and differentiation of Staphylococcus aureus strains containing genes for enterotoxins A, B, and C and toxic shock syndrome toxin 1
... Different synthetic DNA nucleotide sequences were evaluated as gene probes for the specific detection and differentiation of Staphylococcus aureus strains encoding enterotoxins A SEA, B [r] ...
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The minimum transforming region of v-abl is the segment encoding protein-tyrosine kinase.
... Insertions of four amino acids, generated by putting synthetic DNA linkers into various restriction enzyme cleavage sites, abolished transforming activity, indicating that much of the in[r] ...
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Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems
... of synthetic DNA circuitry is an important goal to achieve in the cellular ...on DNA circuitry in serum and serum-supplemented ...of DNA devices using a three-state DNA tweezer ...
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Regulatory motif discovery using a population clustering evolutionary algorithm
... used synthetic DNA sequences to compare the ability of MEME and NestedMICA to identify known regulatory motifs from the JASPAR transcription factor binding site ...These synthetic sequences were ...
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Speculation of Nano gap Sensor for DNA sequencing technology: A Review on Synthetic Nanopores
... presented the ability to read single nucleotide base but with 10% identification error. Others suggested the application of plasmonic structures for unzipping method with a report of simulation results [88, 89]. In their ...
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Synthetic metallomolecules as agents for the control of DNA structure
... protein is made at any point in time. Furthermore, there are a range of special DNA structures, e.g. Holliday junctions, which play essential roles for the existence of life as we know it or as it will become. In ...
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Identification of an l-Rhamnose Synthetic Pathway in Two Nucleocytoplasmic Large DNA Viruses
... genomic DNA was achieved using the gDNA Wipeout buffer of the QuantiTect reverse transcription protocol (Qiagen); this step was followed by reverse tran- scription using the same ...
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AUTORADIOGRAPHY WITH MEIOTIC CHROMOSOMES OF THE MALE NEWT (TRITURUS VIRIDESCENS) USING H3-THYMIDINE
... MONESI (1962) studied the duration of the DNA synthetic period (S phase) prior to meiosis in the male mouse and concluded that there is a gradual lengthening of the S ph[r] ...
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Alpha 1 antitrypsin deficiency caused by the alpha 1 antitrypsin Nullmattawa gene An insertion mutation rendering the alpha 1 antitrypsin gene incapable of producing alpha 1 antitrypsin
... Evaluation of genomic DNA of the index cases and the father of the index cases using synthetic oligonucleotide probes centered at the 353 region and specific for the NUllmatawa mutation [r] ...
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Optimal management of nutrient reserves in microorganisms under time varying environmental conditions
... to synthetic machinery (RNA transcriptase, ribosomes, and the asso- ciated molecular components) and growth machinery (DNA replicase and machinery involved in cell envelope synthesis) there is a dedicated ...
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DNA Polymerase in Virions of a Reptilian Type C Virus
... Imnmunological experiments also to assay the partially purified viral DNA polymerase suggested that the viper virus possesses a group- also contained 2 ug of the synthetic template poly [r] ...
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