T-lymphocyte clones
Identification of two epitopes on the dengue 4 virus capsid protein recognized by a serotype-specific and a panel of serotype-cross-reactive human CD4+ cytotoxic T-lymphocyte clones.
... 1 T-lymphocyte response of a donor who had received an experimental live-attenuated dengue 4 virus (D4V) ...cytotoxic T-lymphocyte clones (CTL) by stimulation with D4 ...CTL ...
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T lymphocyte clones responsive to Shigella flexneri
... Proliferation of clone MC12 in response to EDL and irradiated PBM or a B-lymphoblastoid cell line.. a Clone MC12 2.5 x 104 cells per well with irradiated donor X PBM 5 x 104 cells per we[r] ...
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Vaccinia virus-specific human CD4+ cytotoxic T-lymphocyte clones.
... b Clones established by UV-inactivated vaccinia virus stimulation of PBL from a healthy donor were tested for their ability to lyse uninfected or vaccinia virus-infected autologous B-LCL[r] ...
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Bystander Target Cell Lysis and Cytokine Production by Dengue Virus-Specific Human CD4+ Cytotoxic T-Lymphocyte Clones
... by T cells has not been demonstrated in vivo, the ability of the human hep- atocellular cell line HepG2 to serve as a bystander target for the DV-specific T-cell clones suggests a potential role for ...
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Human CD8+ herpes simplex virus-specific cytotoxic T-lymphocyte clones recognize diverse virion protein antigens.
... Therefore, the B-LCL targets were infected with HSV-2, IL-2 was added to 32 U/ml final concentration, and the cells were incubated overnight in parallel with untreated or acyclovir-treat[r] ...
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Polymorphism of cytotoxic T-lymphocyte clones that recognize a defined nine-amino-acid immunodominant domain of lymphocytic choriomeningitis virus glycoprotein.
... From our results, 6 CTL clones selected at random from the 15 clones that recognized one immunodominant GP epitope possess unique sequences from amino acids 272 to 293, two of the six cl[r] ...
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Cytotoxic T-lymphocyte clones specific for an immunodominant epitope display discerning antagonistic response to naturally occurring Epstein-Barr virus variants.
... There is now convincing evidence that EBV induces a potent CTL response in vivo, yet the virus is able to persist for life in the lymphoid tissues of the immunocompetent host. Earlier studies have suggested that EBV ...
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Persistence of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T-Lymphocyte Clones in a Subject with Rapid Disease Progression
... CTL clones in subject 115, an individual with chronic stable disease and persistent functional CTL ...CTL clones were isolated and maintained by limiting dilution clon- ing performed on either fresh or ...
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Ultrastructural changes during lysis of L929 target cells by class II-restricted influenza virus-specific murine cytotoxic T-lymphocyte clones.
... KO,1t AND YASUHIRO HOSAKA2* Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565,1 and Department of Microbiology, Osaka Unive[r] ...
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Generation of Cytomegalovirus-Specific Human T-Lymphocyte Clones by Using Autologous B-Lymphoblastoid Cells with Stable Expression of pp65 or IE1 Proteins: a Tool To Study the Fine Specificity of the Antiviral Response
... Although recombinant proteins were similarly expressed in the stably transfected BLC stimulator cells, generation of T- cell clones reactive against pp65 was less efficient than against IE1. Such a result ...
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Abnormal cytolytic activity of lymphocyte function associated antigen 1 deficient human cytolytic T lymphocyte clones
... CTL clones and LFA-1- deficient lymphoid target ...CTL clones was 43% of control when tested against a target cell line expressing normal levels of LFA-1 and less than 10% of control when tested against an ...
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Cross-Reactivity against Multiple HIV-1 Epitopes Is Characteristic of HIV-1-Specific Cytotoxic T Lymphocyte Clones
... CTL clones that recognize multiple HIV-1 variants that are highly distinct from their index ...CTL clones (recognizing SL9 Gag 77– 85 and IV9 Pol 464 – 472) cross-recognized the A*02-restricted ...
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Cytotoxic T lymphocyte responses to Ras protein
... CD4+ T lymphocyte clones were raised from a healthy donor with specificity for peptides carrying different amino acid substitutions at residues 12 and ...These T cell clones were either ...
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The Memory Cytotoxic T-Lymphocyte (CTL) Response to Human Cytomegalovirus Infection Contains Individual Peptide-Specific CTL Clones That Have Undergone Extensive Expansion In Vivo
... CTL clones specific to a total of four pp65 peptides, all restricted by three different HLA al- ...these clones from six healthy virus carriers at one to four time points up to 18 months ...CTL ...
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Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV 1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
... naive” T cells and memory CD4 ⫹ and CD8 ⫹ T cells obtained post-cART to generate “DC-stimulated memory” T cells at a dC:T (ratio of DC-primed naive T cell to DC- stimulated memory T ...
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T Lymphocyte responses to murine cytomegalovirus infection
... The data in Chapters 2 and 3 confirmed the usefulness of this model for the generation of MCMV-specific cytotoxicity dependent upon Thyl+, Lyt2+, Class I, H-2 restricted cells, the diffi[r] ...
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Characteristics and functions of Sendai virus-specific T-cell clones.
... The intravenous inoculation of T-cell clones, such as 2H3.B8, 2H3.E8, or 2H3.A5 106 cells per mouse, into mice which had received intranasally a lethal dose of SV 24 h earlier augmented [r] ...
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In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity.
... Virus infectivity was monitored by electroporating the mutant HIV proviral clones into the T4+ lymphocyte cell line A3.01, which has been shown to be sensitive to viral infection and tra[r] ...
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Deficient autologous mixed lymphocyte reaction in Kaposi's sarcoma associated with deficiency of Leu 3+ responder T cells
... phenotype) T cells. Leu-3+ T cells were purified from two patients by depleting Leu-2+ T cells in complement-dependent ...Leu-3+ T cells from both patients demonstrated poor proliferative ...
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Contact of human immunodeficiency virus type 1-infected and uninfected CD4+ T lymphocytes is highly cytolytic for both cells.
... 1 T cells (vMN462) were not lysed ...1 T cells is destroyed after only a few hours and without undergoing significant syncytium ...1 T cells, syncytium formation involving such T-cell leukemia ...
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