TaqMan real-time RT-PCR
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... the real time RT-PCR detec- tion method for H5N6 subtype AIV to prevent and con- trol the spread of H5N6 ...sensitive TaqMan real time RT-PCR method was ...
7
Use of TaqMan Real Time Reverse Transcription PCR for Rapid Detection, Quantification, and Typing of Norovirus
... The TaqMan assay easily and rapidly detected previously identified clusters of GI, GII, and ...assay time by moni- toring amplification of target sequences in real time by fluo- rescence ...
8
Real-time quantitative RT -PCR (Taqman)
... (Advantage RT-for-PCR Kit - Clontech) All reagents listed are provided with the kit a) Quickly thaw each tube in kit and place on ice b) Spin each tube briefly in a tabletop microcentrifuge and return to ...
6
Title: Design and validation of an H5 TaqMan real-time one-step RT-PCR and
... and rapid viral diagnosis of human disease associated with avian influenza A H5N1 23 virus.. Pathogenesis of avian influenza A (H5N1) viruses in ferrets.[r] ...
38
A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... A real-time PCR assay for ...the TaqMan hydrolysis probe, Plasprobe, recog- nizes all species of Plasmodium that infect humans, including ...
7
Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR
... Green PCR Master Mix is covered by ...for real-time PCR) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities ...
17
Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... the Real- GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea), a total of 62 bacterial, 25 fungal refer- ence strains (Table 1), and 115 clinical isolates from various ...
10
Original Article Development of TaqMan real-time PCR for detection of stenotrophomonas maltophilia
... general PCR, these assays has never been used widely due to their defects in accuracy, sensitivity or ...general PCR was more insensitive when it was compared to SYBR Green qPCR [22], yet, the sensitivity ...
8
Detection of ovine milk adulteration using taqman real-time pcr assay
... Food safety, quality and composition have become the subjects of increasing public concern. To prevent fraud and enhance quality assurance, credible analysis of dairy products is crucial. Bovine milk is more widely ...
6
A real time RT PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes
... single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan ...
5
A TaqMan real time PCR assay for Rhizoctonia cerealis and its use in wheat and soil
... Li, W., Sun, H., Deng, Y., Zhang, A. & Chen, H. (2013). The heterogeneity of the DNA ITS sequences and its 20 phylogeny in Rhizoctonia cerealis, the cause of sharp eyespot in wheat. Current Genetics 60, 1-9. 21 22 ...
16
Detection and Genotyping of Varicella Zoster Virus by TaqMan Allelic Discrimination Real Time PCR
... TaqMan PCR does not require postamplification steps, as the formation of PCR product is monitored by real-time mea- surement of reporter dye fluorescence, and thus, there is no need for ...
5
Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... on TaqMan PCR because in our clinical diagnostics all other viruses are identified by this tech- ...and time-consuming procedures that are associated with some of the older methods, since the ...
7
Validating Microarray Data Using RT 2 Real-Time PCR Products
... for real-time PCR. Other methods (such as TaqMan) require an expensive third primer labeled with a dye and a ...Most real-time systems detect and accommodate SYBR Green making ...
7
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
... by real- time RT-PCR using a specific in vitro-transcribed RNA quantification standard as described in Drosten et ...basic RT-PCR reagents (TaqMan Fast Virus 1-Step Master ...
8
quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... a Real-time semi-quantitative TaqMan ® PCR procedure for the determination of the presence of sequence-containing DNA in Rice species DNA extracted from Rice grain ...replicate ...
10
Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... different real-time PCR system, which amplified a 149-bp fragment to detect ...The real- time PCR approach increased the detection of PCV2 samples by 18% over that achieved by ...
5
Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... for ST16, M. hyorhinis strain 211, isolated in 1981, and M. hyorhi- nis strains 142 and 143, isolated in 1999). The MLST primers were designed to amplify only M. hyorhinis sequences, and their speci- ficity was verified ...
8
A TaqMan Real-time PCR System for the Identification and Quantification of Bovine DNA in Meats, Milks and Cheeses
... by PCR amplification of DNA is based on the presence of mamma- lian somatic cells in the milk ( Herman, 2001 ...plex PCR procedures have been developed for species identification in milk, cheese and yogurt ...
10
TaqMan Multiplex PCR Optimization For optimization of Multiplex PCR using 7500/7500 Fast, ViiA 7, and QuantStudio Real-Time PCR Systems
... If the required endogenous control target is available as a primer limited assay, you can begin validating your duplex PCR. However, if it is not, you must limit the primer concentration in the assay yourself. The ...
30