target DNA
Gammaretroviral Integration into Nucleosomal Target DNA In Vivo
... gammaretroviral DNA integration were positively correlated with DNase I-hypersensitive sites in ...nucleosomal DNA is actually favored over naked DNA, raising the question of whether integration ...
9
Key determinants of target DNA recognition by retroviral intasomes
... preferential target- ing of structural chromatin features is most evident for the lenti- and ...to target chromatin-specific features than do either the lentiviruses or gammaretroviruses, with the ...
19
Fast DNA Diagnostic Using Fe3O4 Magnetic Nanoparticles and Light Emitting ZnS/Mn Nanoparticles
... each target DNA containing solution (later called target DNA solution) with different concentrations was bath in boiled water for 5 ...of target DNA solution after ...initial ...
11
Class II Transposons ; also known as Miniature I nverted-repeatsT ransposable Elements or MITE s. Class III Retrotransposons that first transcribe the
... of target DNA are generated footprints (residual DNA sequences) are often left behind after the element is excised Ds are truncated versions of Ac; these can lack a transposase or the inverted ...
11
Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis
... the target DNA to protect Au-nanoprobes against S1 nuclease activity and maintain cy5 enhanced Raman signals was tested us- ing SERS ...the target DNA sequence were gradually cooled down from ...
11
Impedimetric measurement of DNA-DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)
... thiolated DNA probe sequence for the mecA gene and ...mecA target DNA + 10 mM KCl solution for 20 minutes to allow DNA hybridisation, followed by a stringency wash in 1 mM KCl to remove any ...
15
Loop Mediated Isothermal Amplification for Detection of African Trypanosomes
... the target DNA to detectable ...sensitive DNA amplification method in many places, under field conditions and in local clinics and hospitals where cost and environmental restraints prohibiting PCR ...
8
PCR DNA probe assays for identification and detection of Prevotella intermedia sensu stricto and Prevotella nigrescens
... PCR- DNA probe assays for the specific and sensitive identification and detection of ...of DNA probes that we (24) and others (7, 11, 21, 29) had ...genomic DNA of strains belonging to the ...
7
Multiplex target capture with double-stranded DNA probes
... multiplex target capture with dou- ble-stranded, complementary LPPs performed better than single-stranded LPPs and can be built in less than half the time of traditional probe ...duplex DNA have identical ...
8
Detection of Smallpox Virus DNA by LightCycler PCR
... amplified DNA products (204 bp) detectable by FRET analysis with primers and probes whose sequences were homologous to a portion of the sequence of smallpox virus DNA within the HA ...The DNA of each ...
5
Transposon Tn5 target specificity: preference for insertion at G/C pairs.
... A short segment of target DNA is duplicated during transposition, probably the result of staggered nicks made in the target DNA by transposition proteins and repair of the[r] ...
6
DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification
... Mitochondrial DNA has several features that can make it a useful marker for human ...nuclear DNA), mtDNA typing is well suited to biological specimens where DNA fragmentation has occurred or the ...
13
Campylobacter cryaerophila isolated from a human
... First, DNA from the clinical isolate 02811 was labeled with [32P]dATP by the nick translation method and probed for DNA homology with the unlabeled target DNAs of type and reference Camp[r] ...
5
Assessment of the Target Capture PCR Hepatitis B Virus (HBV) DNA Quantitative Assay and Comparison with Commercial HBV DNA Quantitative Assays
... the target-capture PCR assay (Table ...PCR-based target amplification, several points of overlap could be established to obtain a ratio of copies per milliliter to ...follows: target-capture PCR ...
6
Functional analyses of the Neurospora crassa MT a-1 mating type polypeptide.
... idiomorphic DNA: The ability of MT a-1 to bind to spe- cific DNA sequences suggests a mechanism by which it could control the many target genes we presume are required to [r] ...
8
The human T transcription factor: A study of genetics and function
... transcription/translation reaction does not include either a stop codon, or a polyadenylation signal sequence. It seems likely that these om issions m ight effect the com pletion of transcription or translation. Furtherm ...
264
CD4- and Time-Dependent Susceptibility of HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity
... Antibodies and cell lines. An array of MAbs against HIV-1 gp120 (A32, 2G12, PGT126, PGT145, and 3BNC117) and the TZM-bl and CEM.NKr-CCR5 (CEM) cell lines were obtained from the NIH AIDS Reagent Program. The ...
16
Duplex DNA Capture
... heterogeneous DNA mixtures or directly from biological samples have contributed immensely to molecular biology, genomics and the emerging field of DNA ...of DNA isolation and ...of DNA ...
6
ATM controls DNA repair and mitochondria transfer between neighboring cells
... inhibited DNA–PK and ATM to discriminate between their pathways (Additional file 9: Figure ...impaired DNA repair under inhibition [19, 20], we observed a significant, foci reduction of 57% in ATMwt ...
11
Development of a Species Specific Probe for Pythium insidiosum and the Diagnosis of Pythiosis
... A common route of infection by P. insidiosum is via zoo- spores through small cuts or body cavities of individuals who spend time in or drink from stagnant swampy waters or fields. Many fungal species, including other ...
8