Tat peptide
Tat peptide-admixed elastic liposomal formulation of hirsutenone for the treatment of atopic dermatitis in Nc/Nga mice
... significantly lower than those of the liposomal formulations. The slope of the EL/T formulation was much steeper than those of the other formulations. The order of HST penetration at 24 hours was as follows: EL/T . EL . ...
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Tat peptide-decorated gelatin-siloxane nanoparticles for delivery of CGRP transgene in treatment of cerebral vasospasm
... derived Tat peptide-decorated gelatin-siloxane (Tat-GS) nanoparticles via sulfhydryl groups, and we hypothesized that these nanoparticles would be biocompatible as vectors and could enhance gene ...
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Transactivator of transcription (TAT) peptide–chitosan functionalized multiwalled carbon nanotubes as a potential drug delivery vehicle for cancer therapy
... (TAT) peptide was used for noncovalent functionalization of multiwalled carbon nanotubes (MWCNTs), aiming at providing a more efficient drug delivery vehicle for cancer ...The TAT–chitosan-conjugated ...
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Topical ocular delivery to laser-induced choroidal neovascularization by dual internalizing RGD and TAT peptide-modified nanoparticles
... of peptide to ...iRGD peptide until the concentration of iRGD became almost ...of TAT-modified PEG–PLGA (TAT– PEG–PLGA) followed the same ...Free TAT peptide was determined using ...
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Quantum dots: synthesis, bioapplications, and toxicity
... Ruan G: Imaging and tracking of Tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding.. Wei Y: Surface[r] ...
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A TAT-conjugated peptide inhibitor of polo-like kinase 1 for in vivo tumor imaging
... other peptide drugs, its action is limited by the difficulty of intracellular delivery (Malik et ...47–57 TAT fragment is a well-known cell-penetrating peptide, and it is very useful for introducing ...
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InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity
... For in vivo C. albicans infection, a group of C57B/L6 fe- male mice aged 6–8 weeks were injected via lateral tail vein with 1 × 10 5 CFUs of C. albicans in 200 μl sterile saline. One hour later, the mice were injected ...
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The Telomerase-Derived Anticancer Peptide Vaccine GV1001 as an Extracellular Heat Shock Protein-Mediated Cell-Penetrating Peptide
... and TAT in Jurkat T-cells upon co-treatment with additives that can affect the cell entry and intracellular trafficking of CPPs [19], namely heparin (10 mg/ml), methyl-b-cyclodextrin (MbCD), nocodazole (20 mM), ...
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Conjugation of an oligonucleotide to Tat, a cell penetrating peptide, via click chemistry
... It is not fully understood why the reaction between 84 5ʹ-azido-modified oligonucleotide and alkyne-modified Tat peptide did not proceed, however 85 successful formation of oligonucleoti[r] ...
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Synergistic Enhancement of Antitumor Efficacy by PEGylated Multi walled Carbon Nanotubes Modified with Cell Penetrating Peptide TAT
... cell-penetrating peptide TAT modified with PEGylated MWCNTs was developed, characterized, and applied to augment the antitumor activity of DOX to cancer ...the TAT-modified MWCNTs displayed ...
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Cell penetrating peptides selectively targeting SMAD3 inhibit profibrotic TGF β signaling
... of TAT–SH3-2 improved lung histology, reduced inter- stitial fibronectin to essentially basal levels, and showed a dose- dependent diminution in total collagen production induced by BLM, the mutant TAT ...
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Brain delivery of small interfering ribonucleic acid and drugs through intranasal administration with nano-sized polymer micelles
... cell-penetrating peptide-modified polymer ...with Tat peptide in the intranasal administration enables the non-invasive delivery of therapeutic agents to the brain by increasing the transfer of the ...
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Tünnemann, Gisela (2009): Toxicity, uptake and applications of intracellular delivery by cell penetrating peptides. Dissertation, LMU München: Fakultät für Biologie
... the TAT-HA2 pep- tide flooded into living cells and accumulated in nucle- oli; later it was also localized in cytoplasmic vesicles ...of TAT-mediated peptide internaliza- tion, several TAT ...
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Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription
... Virion genomic RNA levels are not altered in Dtat viruses. To determine whether the defects in reverse transcription were due to alterations in HIV-1 RNA encapsidation, we performed RT-PCR on RNA obtained from partially ...
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Modulation of microtubule assembly by the HIV 1 Tat protein is strongly dependent on zinc binding to Tat
... We first investigated the interaction of Tat with tubulin dimers at 20°C in 20 mM NaPi, 10 μM GTP, pH6.5 (PG buffer). This buffer normally allows neither the associa- tion of tubulin nor microtubule assembly at a ...
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Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans
... pUxcs has only one or two extra copy of the gene than S. lividans/pU-xcs-bgl, BGL productivity was drastically increased. It has been reported that the multi-copy vector doesn’t necessarily encourage protein expression ...
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Equine infectious anemia virus tat: insights into the structure, function, and evolution of lentivirus trans-activator proteins.
... Expression plasmids derived from EIAV tat cDNA clones were transfected into mammalian cells to evaluate tat expression and to examine the effects of amino acid deletions on Tat activity.[r] ...
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In vitro nuclear interactome of the HIV 1 Tat protein
... HIV-1 Tat with the SIN3/HDAC and NuRD complexes fur- ther implicates them as potential epigenetic regulators of HIV-1 post-integration latency and suggests how Tat might intersect with epigenetic ...
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Original Article HIV-1 Tat Contributes to Alzheimer’s Disease-like Pathology in PSAPP Mice
... HIV-1 Tat directly modulates polymerization of microtubules; a process which positively correlates with ...thus Tat expression) is in accord with previous studies. This effect of Tat on the ...
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Mechanism of HIV 1 Tat RNA translation and its activation by the Tat protein
... the Tat and the Rev factors are absolutely required for viral gene expression at the transcriptional and post-transcrip- tional levels in infected cells ...HIV-1 Tat is a small basic protein that mainly ...
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