Tendon-derived stem cells
Markers for the identification of tendon-derived stem cells in vitro and tendon stem cells in situ – update and future development
... of tendon-derived stem cells (TDSCs) for the promotion of tendon and tendon-bone junction repair has been reported in animal ...the tendon stem cell niche in vivo ...
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Celastrol improves self-renewal and differentiation of human tendon-derived stem cells by suppressing Smad7 through hypoxia
... The cells were lysed in radio-immunoprecipitation assay lysis buffer and the concentration of protein was deter- mined using the bicinchoninic acid Protein System ...
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Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder
... qRT-PCR was performed as previously described [21]. Briefly, cells were harvested and homogenized for RNA ex- traction with RNeasy mini kit (Qiagen GmbH, Hilden, Germany). Then, mRNA was reverse transcribed to ...
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Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions
... removed from the rats to ensure a minimum margin of 0.5 mm between tendon and muscle or enthesis. The tissue was sectioned into 1 × 1 mm pieces and digested in saline using collagenase (C0130; Sigma-Aldrich Co., ...
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A comparison of the stem cell characteristics of murine tenocytes and tendon derived stem cells
... for tendon is poorly understood, however it is well documented that tendon tissue is unable to heal effectively resulting in painful and debilitating scar tissue, which is unable to perform its nor- mal ...
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Abstract: Mesenchymal Stem Cells derived from bone marrow (hBM-MSCs) are utilized in tendon
... COL1A1 is the major component of tendon tissue (75–85% of the dry mass of tendon), and is the responsible for its mechanical strength [65]. The up-regulation of COL1A1 genes by hBM-MSCs reached 1.5-fold, ...
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Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells
... foreign cells while theoretically preserving the ultrastruc- tural, mechanical, and biochemical motifs of the native tissue ...decellularized tendon and cartilage may serve as the ideal scaffold to promote ...
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Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells
... typical tendon stress–strain curve exhibits three distinct phases, as shown in ...the tendon stretches out to pro- duce normal ...loaded tendon behaves purely elastically, as depicted by the linear ...
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Influence of commonly used pharmaceutical agents on equine bone marrow-derived mesenchymal stem cell viability
... Although the precise mechanism by which MSCs implanted into core SDFT lesions result in improved healing is still unknown, it is logical to deduce that survival with or without further proliferation of implanted ...
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PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells
... movement. Tendon injuries are common and often caused by overstretching the ten- ...stromal cells (BM-MSCs) are difficult to mobilize into the injured sites of tendons ...accelerate tendon ...
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Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon
... the tendon tis- sues were then removed, carefully dissected, cut into pieces, and digested in 3 mg/mL of type I collagenase (Sigma-Aldrich, ...China). Cells were subcultured at 80% confluence. Cells ...
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Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon–bone healing in a rabbit model of anterior cruciate ligament reconstruction
... mesenchymal stem cells (BMSCs) have attracted much interest and have be- come ideal seed cells for tissue engineering because of their multipotentiality and self-renewal potential, as well as their ...
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Harnessing endogenous stem/progenitor cells for tendon regeneration
... biologically derived and FDA-approved hydrogel ...of cells and/or bio- chemical factors. As a blood plasma–derived protein, fibrin gel is biocompatible, biodegradable, and favorable for cell adhesion ...
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Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response
... mal stem cell surface markers, including CD73 and CD146, whereas the hematopoietic marker CD34 was negative ...two cells was tested by CCK-8 assay, and the results displayed that the proliferation rate of ...
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Collagen type I and decorin expression in tenocytes depend on the cell isolation method
... in tendon such as collagen type I, collagen type III, decorin, tenascin-C and fibronectin were ex- pressed continuously by all TLCC of the third cell pas- ...of tendon dis- orders ...
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A comparison of the use of adipose-derived and bone marrow-derived stem cells for peripheral nerve regeneration in vitro and in vivo
... seeded cells, through which blood vessels and other cell types can immigrate and form a new nerve supportive structure [40, ...of cells after being injected into ...
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ADIPOSE-DERIVED STEM CELLS IN TISSUE ENGINEERING: LABORATORY TO BEDSIDE
... The site of tissue harvesting may influence the concentration and function of human ASCs. Lipoaspirates from the inner thigh and lower abdomen may have a higher concentration of MSCs than lipoaspirates harvested from the ...
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Crosstalk between stem cell and spinal cord injury: pathophysiology and treatment strategies
... immune cells and promoting nerve regen- eration and anti-apoptotic effects ...rived cells, as well as Schwann cells ...nerve cells that are degenerated or undergoing necrosis in SCI through ...
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Differences in the cargos and functions of exosomes derived from six cardiac cell types: a systematic review
... T cells via the αvβ6-TGF-β pathway, while other studies have confirmed the involve- ment of TGF-β in the formation and growth of athero- sclerotic ...exosomes derived from human umbil- ical vein ECs or ...
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A Common Suite of Coagulation Proteins Function in Drosophila Muscle Attachment
... target tendon cells (Schnorrer and Dickson 2004; Schejter and Baylies 2010; Schweitzer et ...epidermally derived tendon cells at direct attachment sites (or muscle– tendon ...
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