terminal restriction fragment
Bacterial Diversity in Cases of Lung Infection in Cystic Fibrosis Patients: 16S Ribosomal DNA (rDNA) Length Heterogeneity PCR and 16S rDNA Terminal Restriction Fragment Length Polymorphism Profiling
... by terminal restriction fragment length polymorphism (T-RFLP), length heterogeneity PCR (LH-PCR), and sequencing of individual cloned PCR products to characterize these ...
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Examining Complex Microbial Communities Using the Terminal Restriction Fragment Length Polymorphism Method and Dedicated TRFLP Analysis Software
... that Terminal Restriction Fragment Length Polymorphism analysis could be effectively used to examine microbial communities associated with swine waste lagoon systems (Devine, Chapter ...
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Rapid Identification of Bacteria from Positive Blood Cultures by Terminal Restriction Fragment Length Polymorphism Profile Analysis of the 16S rRNA Gene
... of terminal restriction fragment (TRF) length polymorphisms (T-RFLP) due to 16S ribosomal DNA (rDNA) sequence diversity to rapidly identify bacterial pathogens directly from positive blood ...from ...
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Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences
... Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA G[r] ...
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Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community
... ludwig W., Strunk o., Westram r., richter l., Meier H., Yadhu K., Buchner a., lai t., Steppi S., Jobb G., Forster W., Brettske i., Gerber S., Ginhart a.W., Gross o., Grumann S., Hermann S., Jost r., Konig a., liss t., ...
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Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... The restriction products were purified using filter tubes as described in Mate- rials and ...labeled terminal restriction products from each fungus were mixed to generate a ready-to-use reference ...
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Comparison of microbial populations in the small intestine, large intestine and feces of healthy horses using terminal restriction fragment length polymorphism
... two restriction enzymes were assessed (Cfo and Msp1, Roche Applied Science, Laval, QC, Canada) using a subset of samples and con- ...The restriction enzyme Msp1 resulted in a larger number of T-RF peaks ...
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Chicory (Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs
... ition, terminal restriction fragment length polymorphism (T-RFLP) analysis was carried out on intestinal digesta samples from pigs as previously described ...
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Genetic Architecture of Natural Variation of Telomere Length in Arabidopsis thaliana
... ABSTRACT Telomeres represent the repetitive sequences that cap chromosome ends and are essential for their protection. Telomere length is known to be highly heritable and is derived from a homeostatic balance between ...
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Early development of bacterial community diversity in emergently placed urinary catheters
... We used terminal restriction fragment length polymorphism analysis TRFLP and CFU counts to characterize indwelling urinary catheter microbial community dynamics as a function of catheter[r] ...
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MOLECULAR MARKERS IN WHEAT
... AFLP assays are based on a combination of restriction digestion and PCR amplification. AFLPs caused by mutation or insertion-deletions in a restriction site that create or abolish restriction ...
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A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
... In contrast, genotypic assays that are mostly DNA- based approaches are more accurate and less vulnerable to variations due to growth condition and phenotypic switching. Among the various molecular techniques, the best ...
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Inheritance of restriction fragment length polymorphisms in Agaricus brunnescens.
... Those isolates which differ from their parents by having become homoallelic at a single RFLP locus, or which are distinguished only by a change in size of one restri[r] ...
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Typing of Enterococcus species by DNA restriction fragment analysis
... Total DNA was digested with a suitable restriction enzyme SstI, and the pattern of bands produced after electrophoresis and staining was used for comparison of isolates.. Secondly, DNA d[r] ...
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Behavior of the [mi-3] mutation and conversion of polymorphic mtDNA markers in heterokaryons of Neurospora crassa.
... However, the differences in these mtDNAs can essen- tially be summarized in terms of restriction fragment length polymorphisms (RFLPs) in the EcoRI-5 and -9 restriction fragmen[r] ...
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Detection by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of Human Papillomavirus in Archival Cervical Disease
... Detection by Polymerase Chain Reaction and Restriction fragment Length Polymorphism of Human Papillomavirus in Archival Cervical Disease Detec tion by Polymerase Chain Reaction and Restriction fragmen[.] ...
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Insect Molecular Markers and its Utility- A Review
... Restriction Fragment Length Polymorphism: RFLP is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their ...particular Restriction Endonucleases, ...
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Genetic Divergence of Flax Genotypes (Linum Usitatissimum L.) Utilizing Microsatellite Markers
... (RAPD), Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeat (SSR) have been developed and advanced to assess flax genetic ...
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The distribution and spreading of rare variants in the histone multigene family of Drosophila melanogaster.
... We surveyed the distribution of rare variant restriction sites within and among histone gene arrays of Drosophila melanogaster using restriction fragment length polymorphism[r] ...
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Isolation and characterisation of the 5' region sequence for the bovine ATP citrate lyase gene : a thesis presented in fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... genomic library . A 17 kb clone was isolated . The restriction map of this clone was determined with several enzymes. A part of this clone (9490 base pairs) was sequenced and shown to consist of a 3 kb promoter ...
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