Thaps: thapsigargin
Influence of external calcium and thapsigargin on the uptake of polystyrene beads by the macrophage-like cell lines U937 and MH-S
... promoter thapsigargin was demonstrated to in- crease intracellular Ca 2+ levels by inhibiting Ca 2+ re- uptake from the cytosol by sarco-endoplasmic reticulum ATPases ...
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Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica
... less, thapsigargin was not detected or measured in these ...knowledge thapsigargin production has not been established in in vitro cultures prior to this ...of thapsigargin based on in vitro tissue ...
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Selective depletion of stored calcium by thapsigargin blocks rotavirus maturation but not the cytopathic effect.
... in thapsigargin- treated cells with a set of MAbs against VP7, we found a decreased recognition of this ...in thapsigargin-treated cells could be linked to an incorrect con- formation of VP7 in such ...in ...
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<p>Thapsigargin induces apoptosis in adrenocortical carcinoma by activating endoplasmic reticulum stress and the JNK signaling pathway: an in vitro and in vivo study</p>
... Thapsigargin (TG) has recently emerged as an out- standing anticancer lead compound, and prodrug deriva- tives of TG, such as mipsagargin (G-202), are currently in clinical trials for the treatment of multiple ...
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Measurement of Hepatic Protein Fractional Synthetic Rate with Stable Isotope Labeling Technique in Thapsigargin Stressed HepG2 Cells
... With both glycine and phenylalanine isotope tracers, we found that the protein-bound amino acid enrichments also increased linearly, and FSR values, which were calculate[r] ...
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Palmitic and linoleic acids induce ER stress and apoptosis in hepatoma cells
... with thapsigargin (2 μM), palmitic acid (250 μM), or linoleic acid (250 μM) significantly increased cell death com- pared with the untreated control cells (P < ...
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A γ-Secretase Independent Role for Presenilin in Calcium Homeostasis Impacts Mitochondrial Function and Morphology in Caenorhabditis elegans
... by thapsigargin treatment compared to the modest structural damage ob- served as a result of a global increase in cytoplasmic calcium levels in the egl-19(gof) ...
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Rudy, Anita (2007): Unusual apoptotic signaling pathways in cancer cells induced by cephalostatin. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Caspase-2 activation upon cephalostatin treatment as well as by thapsigargin is independent of an activated caspase-9, as shown using caspase-9-deficient Jurkat cells (Caspase 9-/-,Fig[r] ...
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The role of inositol 1,4,5-triphosphate receptors in mammalian oocytes and preimplantation embryos
... Repetitive sperm-induced Ca^^ transients in mouse oocytes are cell cycle dependent. lonomycin, thapsigargin,[r] ...
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Fusion Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes
... 5 m M Fluo-4 acetoxymethylester (AM) for 20 min or with 4 m M Fura-2 AM for 15 min. FM 1-43 was used at 2 m M in combination with Fura-2. In control experiments, cells were preincubated with CellTracker Green CMFDA ...
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Anti-allergic effects of Marmin, a coumarine isolated from Aegle marmelos Correa : In vitro study
... μM thapsigargin, 10 μM ionomycin, or a combination of 100 nM PMA and 1 μM ionomycin) was added to each well and the plates were incubated at 37°C for 30 ...
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NOTCH inhibits osteoblast formation in inflammatory arthritis via noncanonical NF κB
... Effects of thapsigargin on osteoblast differentiation and bone volume. (A) 2.5-month-old TNF-Tg mice and WT littermates were given thapsigargin (0.4 mg/kg/injection i.p.) or vehicle daily for 4 days. CFU ...
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VASORELAXANT AND ANTIHYPERTENSIVE EFFECTS OF RHUS PENTAPHYLLA (SEARSIA PENTAPHYLLA)
... 6: A EFFECT OF A SINGLE DOSE 0.1mg/ml OF RpAE ON INTACT THORACIC AORTIC RINGS ISOLATED FROM NORMOTENSIVE RATS AND PRE-CONTRACTED WITH PE 10-6 M AND IN THE PRESENCE OF THAPSIGARGIN AND VE[r] ...
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Thioesterase mediated control of cellular calcium homeostasis enables hepatic ER stress
... Disruption of calcium homeostasis by palmitic acid requires the expression of Them2 and PC-TP. Because the incorporation of palmitic acid into the ER membrane induces ER stress in cultured cells by altering ER membrane ...
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The Uranium Lead Geochemistry of the Mount McRae Shale Formation, Hamersley Basin, Western Australia
... Thapsigargin TG induced ER Ca2+ depletion reduced surface expression of mGluR1α, and D208I and Y236I also decreased the receptor trafficking to plasma membrane suggesting the role of Ca2[r] ...
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Production of Infectious Human Cytomegalovirus Virions Is Inhibited by Drugs That Disrupt Calcium Homeostasis in the Endoplasmic Reticulum
... by thapsigargin, CLT, and tunicamycin. We find that thapsigargin and CLT have a profound inhibitory effect on infectious virion ...in thapsigargin, the accumulation of immediate-early and early gene ...
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Identification and characterization of p. nucleorum larvae myosin
... ATPase isolated of P. nucleorum larvae particulate fraction expressed high ATPase activity in the presence of calcium and that activity was significantly inhibited by thapsigargin (Duarte & Coelho, manuscript ...
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Altered calcium signaling in platelets from nitric oxide-deficient hypertensive rats
... to thapsigargin in platelets from control and L-NAME hypertensive rats, in the absence of extracellular calcium (left) and after addition of 1 mM external calcium ...
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Effect of sodium 4 phenylbutyrate on clenbuterol mediated muscle growth
... Although the precise role of the ER in the maintenance of skeletal muscle mass is unknown, ER-stress induced by treatment with thapsigargin causes anabolic resistance in cultured muscle [r] ...
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Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
... activator of NF-kappaB ligand expression in osteoblast. A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation. Thapsigargin modulates osteo- clastog[r] ...
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