The Catalytic Mechanism
CiteSeerX — Selenium as an Electron Acceptor during the Catalytic Mechanism of
... reaction mechanism was recently demonstrated by creating a mutant of human thioredoxin reductase-1 in which the Cys 497 -Sec 498 dyad of the C-terminal redox center was mutated to either a Ser 497 -Cys 498 dyad or ...
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New insights into the catalytic mechanism of aldose reductase : a QM/MM study
... important mechanism is ...the catalytic mechanism of aldose reductase with a QM/MM approach employing this time density functional theory (DFT) as the QM ...the catalytic mechanism that ...
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Identification and characterization of important residues in the catalytic mechanism of CMP‐Neu5Ac synthetase from Neisseria meningitidis
... Here we used crystal structures of CNS and similar enzymes to predict resi- dues of importance in the CNS from Neisseria meningitidis. Nine residues were mutated to alanine, and the steady-state enzyme kinetic parameters ...
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Insight into enzymatic nitrile reduction : QM/MM study of the catalytic mechanism of QueF nitrile reductase
... the catalytic mechanism of the QueF enzymes would expedite rational inhibitor design and enzyme ...the catalytic mechanism of the Vibrio cholerae QueF nitrile reductase by state-of-the-art ...
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The catalytic mechanism of glyceraldehyde 3-phosphate dehydrogenase from Trypanosoma cruzi elucidated via the QM/MM approach
... the catalytic mechanism used by GAPDH involving the G3P substrate from 2D-PMF surfaces obtained through QM/MM MD ...enzyme mechanism proposed from the experimental ...
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Investigation of the Catalytic Mechanism of Lysozyme using Substrate Analogs
... residue, identical to N-acetyl-D-glucosamine but for the C 6 carbon and hydroxyl, can bind to subsite D without distortion and thus actually c~ntribute to the overall binding s[r] ...
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An investigation into the catalytic mechanism of the adenovirus type II proteinase
... 1.2.5 The 23kPa adenovirus proteinase 32 Proteinases are proteins with aininoacids linked in a specific sequence. They have a coiled structure folded into a specific shape and are stabilised by hydrogen bonds between ...
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Computer simulations of the catalytic mechanism of wild-type and mutant β-phosphoglucomutase
... T16P. T16 was shown to be an essential component of the hinge that opens and closes the cap and core domains in L. lactis β-PGM. 8 Its mutation to proline would reduce the ability of the cap to open and close, as proline ...
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The role of spin states in the catalytic mechanism of the intra- and extradiol cleavage of catechols by O-2
... the catalytic cycle, with all accessible spin states and two possible pathways, intradiol and extradiol, were ...reaction mechanism, where again for the L-N 2 H 2 ligand in the critical steps a two-state ...
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Protein mass-modulated effects in the catalytic mechanism of dihydrofolate reductase : beyond promoting vibrations
... As temperature increases, ecDHFR undergoes an initial collapse of the adenosine binding domain (ABD), followed by loss of key tertiary interactions in the loop domain, and unfolding of the remaining secondary structures. ...
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Investigation of the catalytic mechanism of RNase P: the role of divalent metal ions and functional groups important for catalysis
... Heide et al., 1999; Siew et al., 1999). Site-specific functional group modification experiments identified non-bridging phosphate oxygens in helix P4 that, when substituted with sulfur, dramatically inhibit ribozyme ...
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The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis
... Abstract Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k cat = 200 s 1 ). ...
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Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD
... the catalytic mechanism of FKBPs that involves C-terminal rotation around the peptidyl-prolyl bond medi- ated by stabilization of the partially rotated transition state in the hydrophobic binding ...the ...
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Catalytic Mechanism of Amyloid-β Peptide Degradation by Insulin Degrading Enzyme: Insights from QM/MM MP2 Calculation
... The mechanism of IDE catalyzed degrading of Aβ peptides, which is of fundamental importance in the design of therapeutic methods for Alzheimer’s disease, has not been fully ...the catalytic mechanism ...
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Catalytic Methanol Carbonylation
... the catalytic mechanism is normally depicted as proceeding via reductive elimination of acetyl iodide from the rhodium cen- ...the catalytic mechanism, some theoretical studies have also ...
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MELATONIN MEDIATES CLASSIC LACTOPEROXIDASES CATALYTIC MECHANISM
... Here, too, was apparent the formation of compound II with no signs of formation of compound I. However, the formation of this compound was somewhat slower and transition of compound II to transitional intermediate with ...
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Kinetics and Mechanism of the Reaction of Catalytic Dehydroaromatization of Methane
... Thus, catalytic activity of catalysts depends on morphology and size of bentonite ...crystals. Catalytic activity depends also on acidity centers on bentonite obtained from different admixtures forming ...
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The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase
... yeast extracts diminished, indicating an efficient hydrolysis of PAR in vivo (Fig. 2e, Supplementary Fig. 5). Although the first PARG gene was cloned in 1997 15 , to date there are no structural data available for a ...
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Structure and catalytic mechanism of the human histone methyltransferase SET7/9
... NMR studies (see Supplementary Information) indicated that a histone peptide containing mono-methylated Lys 4 was better ordered in complex with SET7/9 than unmodified peptide. We therefore used the products of the ...
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Catalytic Mechanism and Mode of Action of the Periplasmic Alginate Epimerase AlgG
... Significance: This is the first structural characterization of a periplasmic alginate epimerase. Pseudomonas aeruginosa is an opportunistic pathogen that forms chronic biofilm infections in the lungs of cystic fibrosis ...
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