The Golgi
Targeting of a Short Peptide Derived from the Cytoplasmic Tail of the G1 Membrane Glycoprotein of Uukuniemi Virus (Bunyaviridae) to the Golgi Complex
... the Golgi, but interestingly enough, the typical vacuoles seen in cells expressing this peptide were absent ...for Golgi targeting (2), we deleted the tail peptide from the C terminus starting from 10 to 60 ...
12
A GFP tagged version of the pseudorabies virus protein UL56 localizes to the Golgi and trans Golgi network through a predicted C terminal leucine rich helix in transfected cells
... the Golgi compartment localization of pUL56, we constructed a plasmid expressing GFP- Rab6a, a small GTPase, which is a ubiquitous rab as- sociated with membranes of the Golgi complex as well as that of ...
11
Retention and targeting in the Golgi apparatus
... the Golgi stack which, in contrast to the CGN, does not exactly follow the main axis of the Golgi ribbon and thus shows a peeling-off configuration (Rambourg and Clermont, ...(2-4) Golgi cistemae ...
153
The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis
... the golgi compartment as its subcellular localization: “leucin zipper-like transcrip- tional regulator 1” ...a Golgi matrix associated behaviour of LZTR-1 upon BFA treatment and therefore conclude that ...
10
Inhibition of intra-Golgi transport in mitotic extracts
... medial Golgi, and is resistant to the clipping enzyme endoglycosidase H, whose effect is normally readily detectable on protein ...the Golgi enzymes act in the same way with or without their spatial ...the ...
211
Differential Effects of the Putative GBF1 Inhibitors Golgicide A and AG1478 on Enterovirus Replication
... of Golgi-specific BFA resistance factor 1 (GBF1), a regulator of secretory pathway integrity and ...to Golgi vesicles, a process important in transport between the endoplasmic reticulum and Golgi ...
8
Giantin is the major Golgi autoantigen in human anti Golgi complex sera
... These Golgi autoantigens are referred to as giantin/macrogolgin/ GCP372, golgin-245/p230, golgin-160/GCP170, golgin-95/GM130, golgin-97, and golgin-67, with their names based in part on their molecular weights as ...
8
Syntaxin 5-Dependent Retrograde Transport to the trans-Golgi Network Is Required for Adeno-Associated Virus Transduction
... the Golgi apparatus (5, 23– 25), but the significance of this trafficking pathway in AAV trans- duction has not been firmly ...target Golgi apparatus trafficking, such as brefeldin A and the more specific ...
15
Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases in Saccharomyces cerevisiae
... the Golgi and from the Golgi to the plasma membrane, involved in vesicle budding, tethering and fusion, were over-expressed in Clostridium thermocellum endoglucanase (CelA)- and Sacchromycopsis fibuligera ...
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Isolation of an intercisternal matrix that binds medial-Golgi enzymes
... containing Golgi proteins still remain in the cytoplasm of cells treated with BFA (De Lemos-Chiarandini et a i, 1992; Hidalgo et ...these Golgi remnants represent Golgi membranes which have been ...
268
Two Types of Virus-Related Particles Are Found during Transmissible Gastroenteritis Virus Morphogenesis
... To this point we have no data on whether cellular factors are responsible for viral morphological maturation or whether spe- cific virus-associated elements are activated during intracellu- lar transport. A combination ...
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Golgi phosphoprotein 2 in physiology and in diseases
... for Golgi localization were inves- tigated using a panel of GOLPH2 truncation mutants ...The Golgi localization of GOLPH2 was not affected by the deletion of the C-terminal part of the ...
5
Golgi protein localisation in Schizosaccharomyces pombe
... of Golgi retention. The TMD of this enzyme was found to contain Golgi targeting information; replacement of the TMD of ST w ith that of dipeptidyl peptidase IV (DPP IV) a type II plasm a m em brane protein ...
252
Role of microtubules in the synthesis, conversion, and release of (pro)insulin A biochemical and radioautographic study in rat islets
... preformed insulin was progressively inhibited and that of newly synthesized hormone delayed. When the islets were preincubated for 120 min with colchicine, subsequently pulse-labeled with [3H]leucine, and eventually ...
14
Fluorescent labelling of the actin cytoskeleton in plants using a cameloid antibody
... prepared. Agrobacterium tumefaciens was transformed with these constructs and Nicotiana tabacum leaves were infil- trated with the transformed agrobacteria, either singly or with the Golgi marker consisting of the ...
9
A High Copy Suppressor Screen Reveals Genetic Interactions Between BET3 and a New Gene: Evidence for a Novel Complex in ER-to-Golgi Transport
... methods, and incubated with either anti-a-factor (A) or anti- in the Golgi complex (p2CPY), before it is proteolyti- CPY (B) antibody. Lane 1, wild-type; lane 2, sec18-1 (NY432); cally cleaved in the vacuole to ...
9
Ultrastructural Pathology of Golgi Apparatus of Nerve Cells in Human Brain Edema
... that Golgi fragmentation is an early and probably irreversible lesion in neurodegeneration, caused by a variety of mechanisms that may “trigger” ...the Golgi apparatus, a process that involves the tubulin- ...
7
Intracellular Localization of Poliovirus Plus- and Minus-Strand RNA Visualized by Strand-Specific Fluorescent In Situ Hybridization
... the Golgi complex is not the unique origin of the virus-induced ...from Golgi-derived vesicles, as suspected previously for morpholog- ical reasons (13) and as inferred from immunocytochemical studies ...
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Phosphorylation of Golgi Peripheral Membrane Protein Grasp65 Is an Integral Step in the Formation of the Human Cytomegalovirus Cytoplasmic Assembly Compartment
... on Golgi membrane morphology and investigated the role of Golgi membrane reorganization in AC morphogenesis and the production of infectious ...the Golgi apparatus (55, ...the Golgi apparatus ...
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Fragmentation of Golgi complex and Golgi autoantigens during apoptosis and necrosis
... the Golgi complex in HeLa cells is fragmented into scattered vesicles by cas- pases due to interference of Golgi reassembly and abnor- malities of the 65 kDa stacking protein GRASP65 ...the Golgi ...
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