The green fluorescent protein
Influence of Some Point Mutations of Green Fluorescent Protein Gene on Expression Efficiency in <i>Escherichia coli</i> - Volume 7 Number 4 (Aug. 2016) - IJCEA
... Green fluorescent protein (GFP) was discovered as a companion protein to aequorin, the famous chemiluminescent protein from Aequorea jellyfish ...small protein with a molecular ...
5
Dynamics of Nontypical Apoptotic Morphological Changes Visualized by Green Fluorescent Protein in Living Cells with Infectious Pancreatic Necrosis Virus Infection
... of green fluorescent protein (EGFP) was ex- pressed in a fish cell line such that EGFP served as a protein marker for visualizing dynamic apoptotic cell mor- phological changes and for tracing ...
8
Exciton dynamics in solid state green fluorescent protein
... We study the decay characteristics of Frenkel excitons in solid-state enhanced green fluorescent protein (eGFP) dried from solution. We further monitor the changes of the radiative exciton decay over ...
5
Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins
... (2011) Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.. Applied.[r] ...
12
Fluoresceination of Lactobacillus rhamnosus through the expression of green fluorescent protein
... Green fluorescent protein (GFP) is a 27-kDa protein with a pI of ...produce green fluorescence of around 500 ...another fluorescent substance, luciferase, in fireflies, ...
7
Green fluorescent protein as a marker for Pseudomonas spp.
... The development of sensitive methods for observing individual bacterial cells in a population in experimen- tal models and natural environments, such as in biofilms or on plant roots, is of great importance for studying ...
9
Generation of a Recombinant Akabane Virus Expressing Enhanced Green Fluorescent Protein
... The eGFP-AKAV we generated was pathogenic in mice. Mor- tality rates were similar to those seen for AKAV-RG. Although eGFP-AKAV generated smaller plaques, and maximum virus ti- ters were 5-fold lower than those for ...
8
Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
... residual fluorescent activity after the modifi- cation of 79 and 80 amino acids actually point out for the first time that K79 and R80 in this helix are crucial for the fluorescent behaviour of ...
12
Green Fluorescent Protein-Tagged Retroviral Envelope Protein for Analysis of Virus-Cell Interactions
... fusion protein. It was expected that eGFP, as part of a type I membrane protein, would be located both intra- and ...high fluorescent sig- nal intensities, which reflect synthesis and transport of ...
6
FMDV replicons encoding green fluorescent protein are replication competent
... Fig. 4. Time course of FMDV replicon-derived GFP fluorescence. Data from mock- transfected BHK-21 cells are shown (×), together with cells transfected with transcript RNA derived from the pGFP-PAC replicon ( ), ...
6
Green Fluorescent Protein as a Targeting Probe.
... Following the transformation, 5mL of LB broth (Fisher) with 5 µL of ampicillin was inoculated with a single colony from the LB agar plate and it was incubated at 37°C overnight shaking at 300rpm. This 5mL growth was ...
64
Green fluorescent protein as a reporter of prion protein folding
... of protein fusions as reporters of protein folding and solubility has emerged rapidly and includes use of chloramphenicol acetyltransferase (CAT) [59], β -galactos- idase [60,61] and secretion by defined ...
9
Specialized mouse embryonic stem cells for studying vascular development
... Notes: Tumor assays of (A–C) A3-ESC exhibiting endoderm, ectoderm, and mesoderm, respectively. (D–F) B2-ESC exhibiting endoderm, ectoderm, and mesoderm, respectively. The selected A3-ESC images show (A) elongated ...
10
Intercellular Trafficking of VP22, a Herpes Simplex Virus Type 1 Tegument Protein
... the protein through dissolving the cellular membrane causes an artifact that confuses the results and leads to misinterpretation as intracellular translocation of the protein ...trafficking, Green ...
5
Transformation of Epichloë typhina by electroporation of conidia
... ardgrass. Green Fluorescent Protein (GFP) has been uti- lized extensively as a marker to aid in the development of fungal transformation systems and to examine early stages in plant/fungal ...
7
Construction of Various γ34.5 Deleted Fluorescent-Expressing Oncolytic herpes Simplex type 1 (oHSV) for Generation and Isolation of HSV-Based Vectors
... Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the ...
12
Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation
... meric green variant of KillerRed ...monomeric green flavin mononucleotide (FMN)-based genetically encoded photosensitizer, miniSOG [29], and its improved variants singlet oxygen photosensitizing ...
12
Determination of Spontaneous Mutation Frequencies in Measles Virus under Nonselective Conditions
... There is a paradox between the remarkable genetic stability of measles virus (MV) in the field and the high mutation rates im- plied by the frequency of the appearance of monoclonal antibody escape mutants generated when ...
7
QUANTIFICATION OF BIOFILMS IN MULTI-SPECTRAL DIGITAL1 VOLUMES FROM CONFOCAL LASER-SCANNING MICROSCOPES
... the fluorescent in situ hybridisation (FISH) using rRNA- targeted gene probes in combination with confocal laser-scanning microscopy (CLSM) allows to directly analyse the microbial community structure of such ...
6
New insights into enzymatic hydrolysis of heterogeneous cellulose by using carbohydrate-binding module 3 containing GFP and carbohydrate-binding module 17 containing CFP
... fusion protein, GC3, containing a green fluorescent protein (GFP) and a family 3 carbohydrate-binding module (CBM3) that can bind both surfaces of amorphous and crystalline celluloses, we ...
11