The P. pastoris Expression System
Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis
... several expression conditions in parallel and require limited equipment compared to bioreactor-based sys- tems ...the P. pastoris culture every 24 hr allows the control of the amount of nutrients ...
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Cloning and Expression of a Functional Core Streptavidin in Pichia pastoris: Strategies to Increase Yield
... is shown in this work as an alternative for process development. Together, these results show the expression of a functional cStp by the yeast P. pastoris. This novel strain could be used to express ...
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Optimization of the expression of surface antigen SAG1/2 of Toxoplasma gondii in the yeast Pichia pastoris
... for P. pastoris expression system is an important factor to be considered in order to improve the yield of the expressed recombinant ...of P. pastoris strain used or the foreign ...
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A novel methanol-free Pichia pastoris system for recombinant protein expression
... for P. pastoris, since Δgut1 showed signifi- cant Aox activity on glycerol but impaired cell growth ...that P. pastoris might preferen- tially use the phosphorylation pathway for the initial ...
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Expression of lignocellulolytic enzymes in Pichia pastoris
... Multifors system (Infors AG, Bottmingen, Switzerland)) containing 450 ml BSM-media (pH ...phase P. pastoris was grown on glycerol (4%) at ...GXII System, Hopkinton, USA). Standard deviations ...
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Preparation of expression plasmids for Pichia pastoris
... heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression ...Pichia pastoris ...
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Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
... in P. pastoris by sequential Phi29 DNA polymerase-based amplification methods, error-prone rolling circle amplification (RCA) and multiple displacement amplification ...
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Insights on Heterologous Expression of Fungal Cellulases in Pichia pastoris
... using P. pastoris as a host expression system ...using p-nitrophenyl-β-D-glucopyranoside as the substrate ...U/ml), p-nitrophenyl-β-D glucopyranosidase (10-12 U/ml) and ...
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Expression of endoglucanases in Pichia pastoris under control of the GAP promoter
... Pichia pastoris, widely used for heterologous protein expression, can be utilized for fungal endoglucanase ...™ expression system allows for rapid clone selection, and employs the methanol ...
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Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris
... transformation system of 10 µg of linearized DNA in 10 μL TE Buffer (10 mM Tris–HCl, 1 mM EDTA, pH ...competent P. pastoris cells was incu- bated in ice-cold electroporation cuvette of ...
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Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
... 31 protein suitable for crystallography experiments. In Pichia we have observed approximately 0.05 mg of purified protein per 100 milliliters of cell culture, a five-fold improvement over insect cell expressed GLB1. ...
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Expression, purification and evaluation of recombinant L-asparaginase inmehthylotrophic yeast Pichia pastoris: Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast Pichia pastoris: Research article
... the expression and evaluation of hydrolysis activity of native sequence (X12746) encoding for L-asparaginase from Erwinia chry- santhemi NCPBB1125 in the popular expression system Pichia ...in ...
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Expression of Gp63 Gene from NIH Strain of Leishmania major in Pichia pastoris
... Although, expression in baculoviruse-insect cell, as an eukaryotic expression system, enables preparation of the recombinant proteins in reasonable amounts, but with post-translational modifications, ...
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Expression of Functional Influenza Virus RNA Polymerase in the Methylotrophic Yeast Pichia pastoris
... three P proteins were expressed in the same cells transiently after infection of recombinant vaccinia viruses (25, 41, 48) or permanently after integration of all three P protein cDNAs into the chromosome ...
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Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris
... Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthe- sized by the adult kidney and responsible for the regulation of red blood cell ...
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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway
... to P. pastoris NRRL Y-11430 [16]) or ...of P. pastoris with Mut + phenotype ...promoter system in P. pastoris Mut + strains have been summarized by Macauley-Patrick et ...
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Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
... into P. pastoris GS115, respectively. The heterologous expression of ReGal5 with removal of introns and the coding sequence for N-ter- minal transmembrane region predicted by TMHMM ...of P. ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... promoters, P AOX1 and P GAP were tested based on two expression systems, induced and consti- tutive, ...induced system produces active clavMO against the microorganisms ...
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Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris
... of P. pastoris SMD1168H for obtaining Cu/ZnSOD has been demonstrated using a constitutive expression system reaching its highest expression after 96 hours, without the need for any ...
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Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
... For laccase and CDH zymograms, samples were mixed with the same loading buffer as described above without heating; they were incubated at ambient temperature for 15 min and the gel was run. After electrophoresis the gel ...
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