The penicillin-binding domain
Characterization of the Bacillus subtilis Penicillin Binding Protein PBP4
... a Penicillin Binding Protein belonging to the class C of AmpH type whose function remains poorly ...N-terminal penicillin-binding domain have been produced in Escherichia coli and ...
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Cloning, Characterization, and Inactivation of the Gene pbpc, Encoding Penicillin-Binding Protein 3 of Staphylococcus aureus
... C-terminal penicillin- binding domain, presumably responsible for peptidoglycan cross-linking, and an N-terminal non-penicillin-binding do- main whose function remains ...
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Penicillin binding protein 3 of Staphylococcus aureus NCTC 8325 4 binds and activates human plasminogen
... The penicillin binding domain of PBP3 that synthesizes the last steps in S. aureus cell wall polymerization is located outermost from the cell membrane in PBP3, but it is cur- rently not known ...
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The 2.4-A crystal structure of the penicillin-resistant penicillin-binding protein PBP5fm from Enterococcus faecium in complex with benzylpenicillin.
... Results Structure of PBP5fm The crystal structure of PBP5fm was solved at 2.4 Å by MAD using data collected from one crystal of the Se- Met-substituted protein cocrystallized in the presence of benzylpenicillin. The ...
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Glycosyltransferases and Transpeptidases/Penicillin-Binding Proteins: Valuable Targets for New Antibacterials.
... GT domain of the essential PBP2 (Y196D being the most frequent mutation) ...the binding affinity of the polymer for the active site and its premature release, thus yielding glycan chains of reduced ...
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Involvement of the Eukaryote-Like Kinase-Phosphatase System and a Protein That Interacts with Penicillin-Binding Protein 5 in Emergence of Cephalosporin Resistance in Cephalosporin-Sensitive Class A Penicillin-Binding Protein Mutants in Enterococcus faecium
... At this time, we have no compelling data to suggest a specific activity for P 5 AP. It shares little similarity with proteins of known function. Our sequence searches retrieved homologous sequences in Enterococcus ...
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Involvement of the Eukaryote Like Kinase Phosphatase System and a Protein That Interacts with Penicillin Binding Protein 5 in Emergence of Cephalosporin Resistance in Cephalosporin Sensitive Class A Penicillin Binding Protein Mutants in Enterococcus faecium
... At this time, we have no compelling data to suggest a specific activity for P 5 AP. It shares little similarity with proteins of known function. Our sequence searches retrieved homologous sequences in Enterococcus ...
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Involvement of the Eukaryote-Like Kinase-Phosphatase System and a Protein That Interacts with Penicillin-Binding Protein 5 in Emergence of Cephalosporin Resistance in Cephalosporin-Sensitive Class A Penicillin-Binding Protein Mutants in Enterococcus faecium
... At this time, we have no compelling data to suggest a specific activity for P 5 AP. It shares little similarity with proteins of known function. Our sequence searches retrieved homologous sequences in Enterococcus ...
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The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length.
... TP domain. This main trypsin-resistant domain had been identified pre- viously in PBP2a* ...GT domain in full-length PBP2a is therefore susceptible to tryptic digestion, as in the truncated ...GT ...
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In silico analysis of penicillin binding protein 5 as an in hibitory target of beta lactam antibiotics in Enterococcus faecalis
... and domain diagrams of PBP5 showed that it comprised 679 amino acid residues, and the total number of negatively charged residues in it was approximately equal to the total number of positively charged ...
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Variation in penicillin binding protein patterns of penicillin resistant clinical isolates of pneumococci
... These two resistant strains had a common PBP pattern that was completely different from the PBP pattern of the penicillin-susceptible laboratory strain in the number of PBPs detectable b[r] ...
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Penicillin binding protein inactivation by human neutrophil myeloperoxidase
... Pseudomonas aeruginosa, of a class of cytoplasmic membrane enzymes (penicillin-binding proteins, PBPs) found in all eubacteria, that covalently bind beta-lactam antibiotics to their active sites with loss ...
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Whole genome sequencing of penicillin resistant Streptococcus pneumoniae reveals mutations in penicillin binding proteins and in a putative iron permease
... to penicillin in ...to penicillin, the introduction of altered versions of PBPs into a penicillin-susceptible strain by sequential transformation led to strains with a minimal increase in resistance, ...
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EPIgeneousTM Binding Domain Kit A
... GST-binding domain of interest has already been validated, inhibitor titration experiments can be performed directly using the recommended conditions (kit and peptide-biotin concentration) described in the ...
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Characterization of nutritionally variant streptococci by biochemical tests and penicillin binding proteins
... A comparative study of 60 strains of nutritionally variant streptococci (NVS) with 34 strains of Streptococcus mitis and 37 strains of Streptococcus sanguis Il showed the presence of a r[r] ...
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Development of an Immunochromatographic Strip for Simple Detection of Penicillin-Binding Protein 2′
... Laboratory for Antimicrobial Agents, Kitasato University, 5-9-1 Shirokane, Minato-Ku, Tokyo 108-8641, Japan, 1 and Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-Ku, Tokyo 108-8641, ...
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Identification of a reticulocyte specific binding domain of Plasmodium vivax reticulocyte binding protein 1 that is homologous to the PfRh4 erythrocyte binding domain
... high-likelihood binding functional domain from PfRh4 were selected for the FACS-based bind- ing assay with reticulocytes enriched by more than ...specific binding activity with reticulocytes but not ...
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DNA binding properties of a chemically synthesized DNA binding domain of hRFX1
... this domain (76 amino acids) was achieved by the solid phase method, which required optimization of several coupling ...DNA binding site in order to simplify study of the interaction of hRFX1/DBD with ...
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Human herpesvirus 6B origin-binding protein: DNA-binding domain and consensus binding sequence.
... DNA-binding domain by EMSA with in vitro-translated OBP H6B ...DNA- binding domain of OBP H6B mapped between amino acids 482 and 514, and the carboxyl terminus mapped between amino acids 744 ...
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Combinational detection of autolysin and penicillin binding protein 2B genes of Streptococcus pneumoniae by PCR
... On the basis of results from the present study, it appears that class B mutations are present at a high rate in isolates for which the MIC of penicillin is ^ 1.0 m g/ml in Japan. There were very few class A ...
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