Three-dimensional co-culture
Characterization of mesenchymal stem cells and fibrochondrocytes in three-dimensional co-culture: analysis of cell shape, matrix production, and mechanical performance
... Progress toward a tissue-engineered meniscus has shown great promise, but has yet to reach clinical appli- cation [9–13]. Tissue-engineered menisci often lack na- tive biochemical and mechanical properties necessary for ...
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Breast fibroblasts modulate epithelial cell proliferation in three dimensional in vitro co culture
... fibroblast culture are provided in ...0.390). Co-culture of MCF10A cells or MCF10AT cells may enhance expression of IGF II in fibroblasts, as has been reported in fibroblasts co-cultured with ...
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Three dimensional Huh7 cell culture system for the study of Hepatitis C virus infection
... cylindrical culture vessel which reduces shear and turbu- lence associated with conventional stirred bioreactors; therefore, it simulates aspects of microgravity similar to the environment encountered during fetal ...
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Airway branching morphogenesis in three dimensional culture
... of three simple branching modes, repeated in different order throughout lung ...in co-culture with endothelial cells opens the possibility to study important aspects of human lung morphogenesis, such ...
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Comparative analysis of ABCG2+ and ABCG2- retinoblastoma cells in three-dimensional culture
... Cell culture: Retinoblastoma cell lines Y79 (ATCC, Manassas, VA) and WERI-Rb27 (a generous gift from Dr. John Ludlow) were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% calf serum. Both cell ...
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Effect of deforolimus and VEGF on angiogenesis in endometrial stromal cells following three dimensional culture
... M199 culture medium), plated in culture dishes (24 wells) and 15 ul thrombin (50NIH unit/ml in ...5.5% CO 2 and 95% humidity for time period up ...
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Cell Dynamics in Three-dimensional (3D) Culture Environments
... cell culture technique provides a powerful platform for understanding biological mechanism of organism, studying cancer and disease model, researching drugs and methods for curing patients, and opening new avenues ...
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A three-dimensional tri-culture model for the ex vivo study of acute myeloid leukaemia
... The growth and capillary network formation of HUVECs within the applied platform of biohybrid glycosaminoglycan hydrogels has previously been demonstrated. 20 Typically, HSCs are found in the vicinity of vascular ...
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The fibroblast Tiam1 osteopontin pathway modulates breast cancer invasion and metastasis
... Using three different experimental systems, includ- ing three-dimensional (3D) mixed cell spheroid co-cultures of mammary epithelial and fibroblast cells, a 3D orga- notypic culture ...
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Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture
... Type Culture Collection (Rockville, MD, USA), were cultured with Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal-calf serum, 50 U/ml penicillin, 50 µg/ml streptomycin and ...tissue ...
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Differentiation of respiratory epithelium in a 3-dimensional co-culture with fibroblasts embedded in fibrin gel
... that co-culture with fibroblasts might improve the differentiation of respiratory epithelium in a three-dimensional tissue model based on fibroblasts embedded into fibrin ...of culture, ...
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Supercapacitance Performances of Electrodeposited Co(OH)2 /three-dimensional Graphene Nanocomposite
... , where C (F/cm 2 ) is the specific capacitance, I (mA) the the discharge current, Δt (s) the discharge time, ΔV (V) represents the potential drop and the area of electrodeS (cm 2 ) , respectively. Based on above ...
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Organotypic brain slice cultures to model neurodegenerative proteinopathies
... the culture medium or by measuring metabolic activity [12, 16, ...different culture mediums, carbo- gen atmospheres and reducing the thickness of slices some adult BSCs have been reported, however extensive ...
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Recapitulating Parkinson's disease pathology in a three dimensional human neural cell culture model
... For immunofluorescence staining, 2D cultures were fixed with ice-cold methanol for 15 min at − 20°C followed by washing with phosphate buffered saline (PBS) solution containing 0.1% (v/v) Tween-20 (PBS-T; Sigma- ...
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A Riview Paper on Content Based Image Retrieval Technique Using Color and Texture Feature
... form of a query image.CBIR further classified on the basis of features,which is used to retrieve the image from image database. Content could be color, shape, texture or any piece of information, which is needed to ...
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RGO and Three Dimensional Graphene Networks Co modified TIMs with High Performances
... The RGO nanosheets and 3DGNs co-modified ER has been prepared to prepare the TIMs. The advantages of the RGO nanosheets and 3DGNs can give full play to loading the RGO nanosheets on the surface of 3DGNs (by a ...
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Dental and mandibular growth in Papio and Pan
... No observed premature or term chimpanzees (Pan troglodytes) have any deciduous teeth emerged (Siebert & Swindler 1991). The deciduous teeth of chimpanzees and gorillas emerge in the sequence d ii di2 dm i dm2 del ...
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Src-mediated morphology transition of lung cancer cells in three-dimensional organotypic culture
... 3-D culture is that it allows assessment of tumor modulating cues derived from the tumor microenvironment ...3-D culture, which allows investigation of EMT of lung cancer cells, a pivotal step towards ...
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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
... cell culture systems perform poorly as preclinical drug discovery tools, and are not suitable models for investigating solid tumors ...cell culture systems provide neither good glioma stem cell (GSC) ...
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Inventions and Innovations in Preclinical Platforms for Cancer Research
... three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip. Lee, S.-A., Kang, E., Ju, J., Kim, D.-S., and Lee, S.-H., Spheroid-based three-dimensional [r] ...
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