Three-dimensional primary culture
A Bioengineered Three Dimensional Cell Culture Platform Integrated with Microfluidics To Address Antimicrobial Resistance in Tuberculosis
... elements: primary host cells infected with fully virulent bacteria, culturing within a 3D structure that incorporates a physiological extracellular matrix, and pharmacokinetic modeling of drug ...medium ...
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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
... in culture, U87 and primary glioma cells were fixed, dehydrated and embedded in paraffin for H&E staining or dried for SEM ...2D culture plates (Figure 1A) displayed a high degree of similarity ...
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Comparative analysis of ABCG2+ and ABCG2- retinoblastoma cells in three-dimensional culture
... Immunofluorescence staining: Paraffin slides were baked for 60 minutes at 60°C, then incubated step- wise in xylene, alcohol and boiling sodium citrate (pH 6.0). Slides were blocked with commercial blocking solution (Syd ...
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A three-dimensional model of primary bovine endometrium using an electrospun scaffold
... Having established a stromal cell population on the scaffold, the timing of epithelial cell seeding was examined. Seeding of epithelial cells onto the stromal-seeded scaffold was attempted 1 or 7 days after stromal cells ...
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Recapitulating Parkinson's disease pathology in a three dimensional human neural cell culture model
... with primary antibody in blocking solution for 1 h at room temperature under gentle continuous ...Following primary- antibody incubation, cells were washed with PBS-T and incubated with secondary antibody ...
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A three-dimensional tri-culture model for the ex vivo study of acute myeloid leukaemia
... vitro culture models and complex animal models are sophisticated ex vivo models, which rely on our ability to replicate tissue microenvironments in order to sustain the growth of donor ...of primary ...
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A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture
... the three-dimensional morphology of the surface to which they are attached is poorly ...their primary and secondary ...the three-dimensional ...
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Breast fibroblasts modulate epithelial cell proliferation in three dimensional in vitro co culture
... Fibroblasts were derived from mammary reduction speci- mens (NAF) and from primary breast cancers (CAF). The tissues were remnants of diagnostic surgical specimens and were obtained from The University of Alabama ...
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Effect of deforolimus and VEGF on angiogenesis in endometrial stromal cells following three dimensional culture
... Having three passages after isolation, the cells were characterized by flow cytometry for cell surface ...(T-PBS). Primary antibodies were added and incubation was performed for 30 ...
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Three dimensional Huh7 cell culture system for the study of Hepatitis C virus infection
... As expected, the cell adhesion molecules E-Cadherin and β-Catenin were membrane localized both in 2-D and 3-D Huh7 cultures; however, there was a profound decrease in the accumulation of nuclear β-Catenin-containing com- ...
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Src-mediated morphology transition of lung cancer cells in three-dimensional organotypic culture
... 3-D culture resembled the glandular his- tology observed in the tumors formed by the implanted A549 cells in mice (Figure ...3-D culture as reported in a previous study (Figure 1B, inset) ...3-D ...
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Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation
... Aggregation of cells is the pivotal stage in the develop- ment of skeletal tissues and the primary resource from which the skeleton is built and through which the skele- ton is modified ontogenetically [29]. ...
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Airway branching morphogenesis in three dimensional culture
... epithelial culture model using a recently described human basal-like air- way epithelial cell line (VA10) cultured in reconsti- tuted basement membrane (rBM) ...air-liquid culture and have been used to ...
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Prevention of the β-amyloid peptide-induced inflammatory process by inhibition of double-stranded RNA-dependent protein kinase in primary murine mixed co-cultures
... genic mice, and in vitro in Ab42-treated SH-SY5Y neu- roblastoma cells [27,29,38,43]. Moreover, other studies have clearly reported that PKR is involved in the activa- tion of NF- B pathway through phosphorylation of IKK ...
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Cell Dynamics in Three-dimensional (3D) Culture Environments
... cell culture due to its simplicity and homogeneity of the cultured spheroids [75, ...drop culture plate, in which the cell suspension could be hung, and a water reservoir around the plate (Figure ...
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Automated microfluidic cell culture of stem cell derived dopaminergic neurons
... between process management software and one or more devices. SiLA defines common commands per device class and the common device states. SiLA defines the device classes to achieve these functionalities such as an ...
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Relationships Between Prior Experiences, Current Teaching Contexts, and Novice Teachers' Use of Concrete Representation for Mathematics Instruction
... The key challenges in making off-the-shelf tissue-engineered organs include providing sufficient oxygen and nutrient exchange to engineered tissue constructs with pore size larger than 100 μm, selecting a suitable cell ...
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Modulation of platelet derived growth factor receptor expression in microvascular endothelial cells during in vitro angiogenesis
... autophosphorylation. During in vitro angiogenesis induced by 3D-culture, both PDGF receptors were rapidly downregulated. Assessment of cell proliferation showed quiescent cells and PDGF unresponsiveness. We ...
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The fibroblast Tiam1 osteopontin pathway modulates breast cancer invasion and metastasis
... We have previously shown that silencing the Rac ex- change factor Tiam1 in tumor-associated fibroblasts in- duces increased invasion and metastasis in epithelial and cancer cells, using different tissue models with a ...
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Halo Orbits around Sun Earth L1 in Photogravitational Restricted Three Body Problem with Oblateness of Smaller Primary
... massive primary in the three-dimensional restricted three-body ...smaller primary as an oblate spheroid with its equatorial plane coincident with the plane of ...
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