Thymidine Incorporation
Treatment of leukemia with large doses of methotrexate and folinic acid: clinical biochemical correlates
... deoxynucleoside incorporation rates were determined. Thymidine incorporation into DNA was variably ...deoxyuridine incorporation was suppressed during the ...
17
Antibody based targeting of FGFR3 in bladder carcinoma and t(4;14) positive multiple myeloma in mice
... Figure 5 R3Mab inhibits proliferation, clonal growth, and FGFR3 signaling in bladder cancer cells expressing WT or mutated FGFR3S249C.. A Inhibition of [3H]thymidine incorporation by R3M[r] ...
15
Inhibition of lymphoproliferation by hyperlipoproteinemic plasma
... lipoproteins in appreciable amounts. [3H]-Thymidine incorporation correlated well with morphologic evidence of lymphoproliferation. The mechanism of the inhibitory effect of type IV or V ...
6
Amber Mutants of Bacteriophages T3 and T7 Defective in Phage-directed Deoxyribonucleic Acid Synthesis
... A corresponding observation was made at the level of phage-directed thymidine incorporation after mixed infection of nonpermissive cells with DO-amber mutants and heterologous wild type:[r] ...
14
Antibacterial properties of diterpenes and their derivatives : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University
... Thymidine incorporation per microgram protein by MRSA strain MR96/l 64 in the presence of increasing concentrations of totarol.. Glutamine incorporation per viable cell by MRSA strain MR[r] ...
13
Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP
... The results indicate that whole-blood microcultures can be used to evaluate T-lymphocyte responses via assessment of increases in the levels of either ATP or DNA synthesis com- pared to those of unstimulated controls. A ...
5
Stimulation of the proliferation of human bone cells in vitro by human monocyte products with interleukin 1 activity
... [3H]thymidine incorporation and in cell number, we found that human monocytes in culture released factors capable of stimulating the proliferation of osteoblast-like cells derived from human ...
8
Retrobulbar T cells from patients with Graves' ophthalmopathy are CD8+ and specifically recognize autologous fibroblasts
... Proliferation [3H]thymidine incorporation of T cell lines in response to stimulation with irradiated autologous retrobulbar fibroblasts U, irradiated autologous retrobulbar fibroblasts i[r] ...
7
Low density lipoprotein enhances the cellular action of arginine vasopressin in rat glomerular mesangial cells in culture
... [3H]thymidine incorporation, an effect significantly greater in the presence of 10 micrograms/ml ...[3H]thymide incorporation and mitogen-activated protein kinase were not altered by 10 micrograms/ml ...
9
PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
... Immunoaffinity chromatography was used for purification of GM-CSF. A column was constructed which anti-GM-CSF was coupled to CNBr-activated Sepharose 4B. The renatured protein was loaded on the column and eluted by the ...
6
The angiotensin AT2 receptor mediates inhibition of cell proliferation in coronary endothelial cells
... Angiotensin II (ANG II) is known to be a potent growth promoting factor for vascular smooth muscle cells and fibroblasts but little is known about its influence on growth in endothelial cells. We studied the effects of ...
8
Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and Man
... The plasmin digests of hGH retained all the metabolic actions of the native hormone in the rat: a to promote growth in hypophysectomized rats, b to stimulate thymidine incorporation into[r] ...
12
Transforming growth factor beta regulates thyroid growth Role in the pathogenesis of nontoxic goiter
... tritiated thymidine incorporation in cultured thyroid follicular cells, but inhibited, at a concentration of 10 ng/ml, the growth stimulatory influence of insulin-like growth factor I, epidermal growth ...
8
Anti-proliferative activity of oral anti-hyperglycemic agents on human vascular smooth muscle cells: thiazolidinediones (glitazones) have enhanced activity under high glucose conditions
... of thymidine kinase and thus the mechanism remains ...radiolabel incorporation into vSMC proteoglycans (Nigro and Little, unpublished observations) so it is possible that these agents also per- turb ...
9
Decreased growth inhibition by recombinant gamma interferon is associated with increased transforming growth factor Α production in keratinocytes cultured from psoriatic lesions
... However, subsequent to the first report, it was suggested that [^*H]-thymidine incorporation by cultured keratinocytes may not accurately refiect keratinocyte proliferation.** Another re[r] ...
15
Effect of transfer factor on lymphocyte function in anergic patients
... Before transfer factor, none of the patients had delayed skin responses to any of the natural antigens studied. Their lymphocytes did not produce MIF after exposure to antigens in vitro and only one patient showed ...
12
Biochemical mechanisms in the Killmann experiment: critique of the deoxyuridine suppression test
... of thymidine kinase activity by both mebolites further decreases the specific radioactivity of [3H]dTMP and ...the incorporation of [3H]deoxyadenosine and [3H]deoxyguanosine into DNA in these ...
13
Role of local prostaglandin synthesis in the modulation of proliferative activity of rat colonic epithelium
... the incorporation of [3H]thymidine (dThd) into DNA in vivo was increased approximately twofold over control in mucosal scrapings from distal colon, and approximately threefold over control in the ...
12
Nature and specificity of the immune response to collagen in type II collagen induced arthritis in mice
... incorporation by mononuclear cells cultured in the presence of collagen. The maximal proliferative response to collagen occurred at 2 wk after immunization. Equally good incorporation of label occurred when ...
12
Effect of β-Propiolactone Inactivation of Polyoma Virus on Viral Functions
... T-antigen production, polyoma-RNA synthesis, induction of host DNA synthesis measured by incorporation of labeled thymidine into the cell culture, and in vitro transforming ability were [r] ...
6