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Time-Lapse Imaging

Genetic mosaics and time lapse imaging identify functions of histone H3 3 residues in mouse oocytes and embryos

Genetic mosaics and time lapse imaging identify functions of histone H3 3 residues in mouse oocytes and embryos

... During development from oocyte to embryo, genetic programs in mouse germ cells are reshaped by chromatin remodeling to orchestrate the onset of development. Epigenetic modifications of specific amino acid residues of ...

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Muscle satellite cell proliferation and association: new insights from myofiber time-lapse imaging

Muscle satellite cell proliferation and association: new insights from myofiber time-lapse imaging

... Time-lapse imaging is unlikely to replace analysis of fixed cell populations for many practical ...the time- and labor-intensive nature of the imaging and analysis, plus the difficulty ...

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3D time lapse imaging of polygonal patterned ground in the McMurdo dry valleys of Antarctica

3D time lapse imaging of polygonal patterned ground in the McMurdo dry valleys of Antarctica

... the time-lapse imaging for this paper was limited to one site there is significant scope for future work at multiple locations to confirm the results seen ...using time lapse surveying ...

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Use of time lapse imaging and dominant negative receptors to dissect the
steroid receptor control of neuronal remodeling in Drosophila

Use of time lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila

... length during each 20-minute interval, either extending, shortening or branching. In instances in which we could follow the regression of individual axon branches, we saw only retraction and no sign of branch ...

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Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms

Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms

... vivo time-lapse imaging of scl isoform- specific reporter transgenic zebrafish lines, we show that prior to EHT scl- β is selectively expressed in hemogenic endothelial cells, a unique subset of VAE ...

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BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo

BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo

... vivo time-lapse imaging of Xenopus retinal ganglion cell (RGC) axon arbors showed that BDNF increases arbor complexity, thereby increasing the number of presynaptic sites in the more elaborate axons, ...

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Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance.

Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance.

... functions in the CNS, for example in the maintenance of normal ionic concentrations and neurotransmitter levels in the extracellular space [18,19]. Consistent with these roles, astrocytes display high levels of membrane ...

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Segmentation of the vertebrate hindbrain: a time lapse analysis

Segmentation of the vertebrate hindbrain: a time lapse analysis

... overlapping time- lapse sequences, starting with 6-8 ss embryos (n=12) and 9-11 ss embryos (n=12); each sequence contained 10-15 h of recorded time-lapse video imaging of hindbrain ...

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The Arp2/3 complex, UNC-115/abLIM, and UNC-34/Enabled regulate axon guidance and growth cone filopodia formation in Caenorhabditis elegans

The Arp2/3 complex, UNC-115/abLIM, and UNC-34/Enabled regulate axon guidance and growth cone filopodia formation in Caenorhabditis elegans

... and time-lapse imaging of growth cones during out- growth, we show that the Arp2/3 complex acts cell auton- omously in the initiation of new growth cone filopodia during growth cone outgrowth, ...

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Dissecting the molecular mechanisms of drosophila border cell migration using time-lapse live cell imaging

Dissecting the molecular mechanisms of drosophila border cell migration using time-lapse live cell imaging

... the imaging is performed and the fluorophores ...Having imaging medium that can support long time-lapse imaging without deterioration enabled data from the first and second halves of ...

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Angiopoietin 2 signaling plays a critical role in neural crest cell migration

Angiopoietin 2 signaling plays a critical role in neural crest cell migration

... vivo time-lapse imaging experiments with control (n = 10), Ang2-FL (n = 8), or Ang2-shRNA (n = 4) elec- troporated neural crest and tracked single neural crest cells in the r4 migratory ...

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Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo

Transient axonal glycoprotein-1 (TAG-1) and laminin-α1 regulate dynamic growth cone behaviors and initial axon direction in vivo

... vivo time-lapse imaging of growth cone behaviors reveals that although there are some similar effects of TAG-1 or laminin-α1 loss of function, there are also distinct and significant ...

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Complement-independent bystander injury in AQP4-IgG seropositive neuromyelitis optica produced by antibody-dependent cellular cytotoxicity

Complement-independent bystander injury in AQP4-IgG seropositive neuromyelitis optica produced by antibody-dependent cellular cytotoxicity

... by time-lapse imaging, we found that NK cells sequentially kill CHO-AQP4 cells followed by bind- ing to and killing of nearby null cells, and that integrin blockade with an RGD peptide reduced ...

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Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement

Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement

... performed time-lapse imaging to quantitatively assess the dynamics of actomyosin ring assembly and contraction in cdc8-S125A and cdc8-S125E mutants, using Rlc1-3GFP and Atb2- mCherry as markers of ...

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Time lapse: A glimpse into prehistoric genomics

Time lapse: A glimpse into prehistoric genomics

... ‘Time lapse’ - to most of the audience - conjures up time-lapse imaging of human embryos; the title that you see above was thus deliberately mischievous as the talk had nothing to do ...

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Transient localization of the Arp2/3 complex initiates neuronal dendrite branching in vivo

Transient localization of the Arp2/3 complex initiates neuronal dendrite branching in vivo

... (F) Time-lapse imaging of differentiating class IV ddaC neurons of second instar larvae expressing Arp3-GFP (green) and mCD8cherry (magenta) imaged every 60 ...Three-minute time lapse ...

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OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation

OSCILLATOR: A system for analysis of diurnal leaf growth using infrared photography combined with wavelet transformation

... Additional file 4: Figure S4. Schematic representation of the different steps of OSCILLATOR. (a) Hardware consisting of cameras which are connected to a dedicated laptop are controlled by Nikon camera control software. ...

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Automated Dynamic Cellular Analysis in Time Lapse Microscopy

Automated Dynamic Cellular Analysis in Time Lapse Microscopy

... On the other hand, for the cell phase classification evaluation, we used sequences of CHO-K1 (Chinese Hamster Ovary Cell) microscopic images of 11 hours long (1000 frames, 1.5 ~ 2 fps). These images were captured with ...

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Time lapse inversion of one dimensional magnetotelluric data

Time lapse inversion of one dimensional magnetotelluric data

... or time-lapse inversions using 2D MT codes as approximations, it has previously been shown that simultaneous inversion results in a model with fewer artefacts and stronger constraints on areas of ...

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Through the Looking Glass: Real Time Imaging in Brachypodium Roots and Osmotic Stress Analysis

Through the Looking Glass: Real Time Imaging in Brachypodium Roots and Osmotic Stress Analysis

... Three days-old seedlings having roots were mounted into wells for the top and bottom imaging. Time Lapse Growth Curve Analysis in normal conditions and Root growth under PEG 137.. The g[r] ...

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