tissue sections
A sensitive non-radioactive in situ hybridization method for the detection of chicken IgG γ-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections
... of tissue morphology. Generally paraffin sections provide better cytological resolution than frozen ...the tissue sections must be fully dried (at 40°C overnight) on the slides to provide ...
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Factors affecting the yield of microRNAs from laser microdissectates of formalin fixed tissue sections
... RPCI. Tissue blocks were cut on a CUT4055 rotary microtome (Triangle Biomedical Sciences ® , Durham, NC) into 8 μ m-thick sections, which were placed on glass slides covered with a PEN membrane (Leica ® , ...
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Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization
... air-dried. Tissue sections were covered with a hybridization mixture containing 11 µl distilled water, 20 µl 20× saline-sodium citrate (SSC) buffer, 50 µl forma- mide, 5 µl herring sperm, 2 µl 50× ...
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In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections
... Post hybridization, staining, and permanent mounting Slides were removed from the 42 °C hybridization oven and dipped in 2× SSPE to aid in coverslip removal. Once coverslips were removed slides were washed in 2× SSPE for ...
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Ki67 expression in invasive breast cancer: the use of tissue microarrays compared with whole tissue sections
... using tissue microarrays (TMA) platform [14] [15], although it remains unclear as to their validity and comparison with assessment in whole tissue sections ...
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Optical and digital methods for the analysis of microscopic tissue sections
... All twelve scans are over prostate tissue sections and the table shows that points with a high gradient magnitude are far more common than points with a high Laplacian value.. In contras[r] ...
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Nuclear size measurement for distinguishing urothelial carcinomas from reactive urothelium on tissue sections
... One helpful feature for detecting cytologic atypia is nucleomegaly of urothelial cells. Because of the lack of reliable reference urothelial cells in tissue sections, lym- phocytes are often used for ...
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Usefulness of molecular biology performed with formaldehyde-fixed paraffin embedded tissue for the diagnosis of combined pulmonary invasive mucormycosis and aspergillosis in an immunocompromised patient
... Immunocompromised patients who develop invasive filamentous mycotic infections can be efficiently treated if rapid identification of the causative fungus is obtained. We report a case of fatal necrotic pneumonia caused ...
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Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing
... wound tissue samples were fixed in 4% formaldehyde solution at 4°C, dehydrated with a graded series of ethanol solutions, embedded in paraffin, and sequentially sectioned at 4 μm ...wound tissue ...
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Developmental changes of lymphoid tissue in Harderian gland of the laying hens
... age. Tissue sections were stained with haematoxylin and eosin, periodic acid-schiff (PAS) and methyl green-pyronin ...lymphoid tissue is colonised by three types of cells: heterophils, lymphocytes ...
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Fatal Cerebral Phaeohyphomycosis Due to Curvularia lunata in an Immunocompetent Patient
... in tissue sections is possible; definitive identi- fication of species of a fungal organism based on morphology in tissue, however, is not always ...in tissue sections as 2- to 6- ...
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Protective Effects of α Tocopherol against Brain Tissue Damage Induced by Intracerebral Hemorrhage in SD Rats
... brain tissue morphology after intrace- rebral hemorrhage (ICH), cerebral edema, or the expression of Bax and Bcl-2 ...brain tissue sections of rats from different groups showed that brain ...
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Rheumatoid arthritis synovium contains plasmacytoid dendritic cells
... synovial tissue. Sections of frozen normal human synovial tissue (a, b, e) or synovial tis- sue from a patient with untreated active RA (c, f, g) or formalin-fixed sections from a patient with ...
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Synovium as a source of increased amino terminal parathyroid hormone related protein expression in rheumatoid arthritis A possible role for locally produced parathyroid hormone related protein in the pathogenesis of rheumatoid arthritis
... synovial tissue specimens. Synovial tissue samples, collected under sterile conditions and kept at 4 8 C for , 1 h before processing, were obtained from patients with RA ( n 5 9) or osteoarthritis (OA) ( n ...
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Hyper stain inspector : a framework for robust registration and localised co expression analysis of multiple whole slide images of serial histology sections
... In this paper, we present a fast method for registration of multiple large, digitised whole-slide images (WSIs) of serial histology sections. Through cross-slide WSI registration, it becomes possible to select and ...
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Sites of synthesis of urokinase and tissue type plasminogen activators in the murine kidney
... on tissue sections and in situ hybridizations, we have explored the cellular distribution of urokinase-type (u-PA) and tissue-type (t-PA) plasminogen activators and of their mRNAs in developing and ...
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Reovirus infection and tissue injury in the mouse central nervous system are associated with apoptosis.
... and tissue damage are restrict- ed to distinct regions within the neonatal mouse ...and tissue injury, brain tissue sections from T3D-infected mice were either stained with cresyl violet to ...
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Autologous adipose-derived regenerative cell therapy modulates development of hypertrophic scarring in a red Duroc porcine model
... paraffin. Tissue sections were subjected to an antigen retrieval step then incubated with primary polyclonal rabbit alpha-smooth muscle actin (α-SMA) (5 μg/ml, Abcam, Cambridge, MA, USA) ...controls, ...
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Detection of colorectal dysplasia using fluorescently labelled lectins
... Lectin binding to fixed tissue sections from human colon. Next, binding of fluorescently-labelled WGA and other lectins to the luminal surface epithelium of fixed human colon tissue sections ...
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Characterization of tau prion seeding activity and strains from formaldehyde-fixed tissue
... frozen tissue was limited to gross ...adjacent tissue slices using anti-tau AT8 staining, we easily de- tected tau seeding activity, even in fixed tissue sections with a minimal AT8 ...fixed ...
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