tissue slices
Regulation of Renal Citrate Metabolism by Bicarbonate Ion and pH: Observations in Tissue Slices and Mitochondria
... in tissue slices and isolated kidney ...in slices of renal cortex and in kidney mitochondria when [HCO 3 - ] and pH are increased within the physiologic range (pH ...liver slices when a ...
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Reproducible proteomics sample preparation for single FFPE tissue slices using acid-labile surfactant and direct trypsinization
... tonsil tissue slices, derived from two patients, with different standard histologi- cal stainings and analyzed them with the DTR ...unstained slices or slices stained with hematoxylin and ...
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Validation and reproducibility of computerised cell viability analysis of tissue slices
... Fifteen slices were selected with varying proportions of green and red cells. Three copies of each image were made with one being the original image, one a mirror image, and one rotated 180° to make it difficult ...
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Age dependent changes in the exocytotic efficacy in Kir6 2 ablated mouse pancreatic β cells
... Pancreatic slices were prepared as previously described ...thick slices using vibratome (VT 1000 S, Leica, Nussloch, ...the tissue slices were kept in ice-cold ECS, carbogenated with 95% oxy- ...
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Pathways of glutamine and organic acid metabolism in renal cortex in chronic metabolic acidosis
... in tissue slices of renal cortex from chronically acidotic and alkalotic littermate ...by slices from acidotic animals providing further evidence for the similarity of the metabolic responses seen in ...
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Assessment of long-term cultivated human precision-cut lung slices as an ex vivo system for evaluation of chronic cytotoxicity and functionality
... lung slices in 1994 [8], optimizations have been made to reproducibly prepare very thin tissue slices of defined ...lung tissue [16–18], these studies only focused on limited endpoints to ...
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Identification and Characterization of Bovine Mammary Peptide Transporters in Response to Tripeptide and Lactogenic Hormone Treatment
... mammary tissue slices and cultured epithelial cells, and the effects of tripeptide and lactogenic hormones on PepT1 and PepT2 mRNA abundance were also analyzed in cultured mammary epithelial ...
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Original Article Toponostics of invasive ductal breast carcinoma: combina- tion of spatial protein expression imaging and quantitative proteome signature analysis
... 53 tissue slices from formalin-fixed and paraffin-embedded tumor and control tissue samples from ten patients and fourteen ...breast tissue samples were investigated by computer-assisted ...
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Periostin: a promising target of therapeutical intervention for prostate cancer
... Furthermore, to determine the effects of sliencing Periostin on LNCap cells in vivo, down-regulated Peri- ostin LNCap cells, normal LNCap cells and control GFP LNCap cells were implanted into the oxter of the nude mice. ...
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Pyruvate carboxylase is critical for non–small cell lung cancer proliferation
... Warburg’s tissue slices (25) that we have introduced enables detailed ex vivo analysis of patient-specific tumor metabolism (Figure 2 and Supplemental Figure 2), while maintaining most of the tissue ...
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Optimal sampling of MRI slices for the assessment of knee cartilage volume for cross sectional and longitudinal studies
... MRI slices and has high repro- ducibility with coefficients of variation of 2–3% [12] and has been used in both cross sectional and longitudinal studies of OA ...thick slices to reduce the post-image ...
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Semi-automated segmentation and quantification of adipose tissue in calf and thigh by MRI: a preliminary study in patients with monogenic metabolic syndrome
... transaxial slices comprising a 51 mm section obtained in both the mid-calf and mid-thigh ...adipose tissue contours within the lower limb segment from a user-defined seed ...adipose tissue was then ...
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Characterisation of androgen metabolism and 5α-reductase activity in human prostate cells in vitro
... in slices of BPH tissue demonstrated that although DHT concentration in BPH tissue is higher than normal tissue, there is no difference in the rate of DHT formation in BPH tissue ...
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Incorporating outlier detection and replacement into a non parametric framework for movement and distortion correction of diffusion MR images
... Signal loss (dropout) affecting a whole slice, or a large connected region of a slice, is frequently observed in diffusion weighted images, leading to a set of unusable measurements. This is caused by bulk (subject or ...
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Significance of MDR1 and multiple drug resistance in refractory human epileptic brain
... assess tissue viability throughout the ...The slices were then fixed in 4% formalin in PBS overnight at 4°C and cryoprotected by 30% ...µm slices were cryosectioned from each 500 µm slice and ana- ...
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Mapping breast tissue types by miniature radio-frequency near-field spectroscopy sensor in ex-vivo freshly excised specimens
... 10 tissue types, as assessed from the data at 4, 10 and 30 MHz, are presented in ...1253 tissue measurements which had feature sizes above ...relative tissue compositions of these samples. Only ...
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Enhanced Cytomegalovirus Infection of Developing Brain Independent of the Adaptive Immune System
... coronal slices at the widest part) was 6 mm wide, the P12 brain was 11 mm wide, and the adult brain was 13 mm ...thick slices of the brain inoculated in vitro, infection of cell bodies was restricted to the ...
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Spontaneous breathing with airway pressure release ventilation favors ventilation in dependent lung regions and counters cyclic alveolar collapse in oleic acid induced lung injury: a randomized controlled computed tomography trial
... Controlled mechanical ventilation in the supine position causes larger tidal excursions of the diaphragm in its upper, anterior part than in its dorsal segment [12]. This is due to higher abdominal pressure in the ...
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Degradation of cartilage proteoglycan by human leukocyte granule neutral proteases a model of joint injury I Penetration of enzyme into rabbit articular cartilage and release of 35SO4 labeled material from the tissue
... The present work was undertaken to explore the effect of two purified neutral proteases derived from human peripheral blood polymorphonuclear leukocytes (PMN) on articular cartilage as a model of joint injury. Human ...
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