Transformation efficiency
Genome alterations associated with improved transformation efficiency in Lactobacillus reuteri
... plasmid transformation to induce mutations in trgA, led us to hypothesize that mutations in trgA improve the ability of LR 6475 to take up plasmid DNA by inactivating the activity of the ...their ...
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COMPARISON OF TRANSFORMATION EFFICIENCY OF FIVE AGROBACTERIUM TUMEFACIENS STRAINS IN NICOTIANA TABACUM L.
... mediated transformation has been widely used for research in plant molecular biology and for genetic improvement of crops exploiting its tremendous ability to transfer foreign DNA to ...the transformation ...
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Characterization of Avirulent TnphoA Mutants in Agrobacterium tumefaciens to Enhance Transformation Efficiency
... Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane protein. Agrobacterium strain A6007 was mutagenized with E. coli strain mm294A plasmid pRK609 ...
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Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula
... The R108 ecotype has a much higher transformation efficiency as compared to the A17 ecotype [6]. The R108 ecotype is derived from the same cultivar as the A17 ecotype, but as a result of multiple rounds of ...
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The effect of different antibiotics on the elimination of Agrobacterium and high frequency Agrobacterium mediated transformation of indica rice (Oryza sativa L )
... The transformation efficiency obtained using this method was around ...high efficiency of transformation obtained lead us to conclude that the method established in this study is an effective ...
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Assessment of plant transformation technology of maize
... higher transformation efficiency and quality of ...mediated transformation and particle ...like transformation efficiency, copy number, expression level of GUS gene and its ciency and ...
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Optimizing agrobacterium-mediated transformation parameters of melastoma decemfidum ROXB EX JACK
... mediated transformation parameters for ...the transformation efficiency in various plant ...The transformation efficiency was determined based on the percentage of positive transformed ...
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High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes
... regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic ...genetic transformation of cauliflower employ‑ ing the process of ...(72% ...
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An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L by using stem internodes as explants
... low efficiency of adventitious shoot and root formation on a selective medium containing additional compounds for Agrobacterium ...reproducible transformation and regeneration of ...
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The Study of High Current Charging and Sawtooth Wave Equalization Method for Ni-MH Capacitance Power Battery
... At present, electric vehicle chargers are mainly divided into three categories according to their working principles [10]-[15]: the first type of charger is composed of power- frequency transformer, uncontrollable ...
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Development of Transgenic Tomato Expressing Antimicrobial Peptide Gene through Agrobacterium-Mediated Transformation
... The effect of bacterial density and duration was studied it was found that 0.4 OD with 1 min of co-cultivation duration gave highest transformation efficiency. Increasing the bacterial cell density and ...
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Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
... transformation efficiency depending on the location of the integration sites within the ...the transformation efficiency in A2842 and to some extent in A0159, but has no detectable influence ...
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High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
... Agrobacterium-mediated transformation of ...the transformation efficiency more than ...the transformation efficiency of five Agrobacterium strains was evaluated in Nicotiana tabacum ...
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The Introduction of Transgenes to Control Blackheart in Pineapple: Biolistics Vs Agrobacterium Transformation, in 'Contributing to a Sustainable Future'
... Abstract. Techniques for the introduction of transgenes to control blackheart by particle bombardment and Agrobacterium co-transformation have been developed for pineapple cv. Smooth Cayenne. Polyphenol oxidase ...
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Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies
... plant transformation protocol is thus crucial for functional studies and crop improvement ...mediated transformation for developing transgenic chickpea ...the transformation efficiency ...
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Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci
... the efficiency of recombination can be increased by several orders of magnitude by using I-SceI sites at the target locus ...cloning efficiency of large DNA fragments into the CRIM plasmids prior to ...
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Multiple Effects of an Anti-Human Immunodeficiency Virus Nucleocapsid Inhibitor on Virus Morphology and Replication
... Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis.. High efficiency transformation of[r] ...
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STUDY REGARDING EFFICIENCY OF INDUCED GENETIC TRANSFORMATION IN BACILLUS LICHENIFORMIS WITH PLASMID DNA
... Genetic transformation through electroporation (Phillip ...genetic transformation of the competent cells with plasmid DNA using MicroPulser electroporator from BIO-RAD, ...
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Regulation of m4 muscarinic receptor gene expression
... Immunological detection of muscarinic receptor subtype proteins (ml-m5) in rabbit peripheral tissues. High efficiency transformation of E.coli. by high voltage electropo[r] ...
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INTEGRATION EFFICIENCY IN DNA-INDUCED TRANSFORMATION OF PNEUMOCOCCUS. II. GENETIC STUDIES OF MUTANT INTEGRATING ALL THE MARKERS WITH A HIGH EFFICIENCY
... markers was determined in both strains CE, and 401. The experimental method used was very similar to the one used in the experiments above, except that the transformed bacteria we[r] ...
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