Transient transfection
Liposome and polycation-transferrin-conjugate mediated delivery of DNA for the transient transfection of haemopoietic cell lines
... toxic transfection protocols would be ...of transient transfection of non-adherent haemopoietic cells are problematic because of the toxicity observed and the small proportion of the cells ...
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Transient transfection of human CDNF gene reduces the 6-hydroxydopamine-induced neuroinflammation in the rat substantia nigra
... Figure 5 Expression of cerebral dopamine neurotrophic factor (CDNF) by astrocytes and astrocyte-like NG2 cells in the substantia nigra pars reticulata (SNr). Representative merged confocal micrographs showing ...
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Regulation of transcription from the hepatitis B virus large surface antigen promoter by hepatocyte nuclear factor 3.
... the transient-transfection analysis in ...in transient-transfection analysis when the en- hancer I sequence is removed from the context of the HBV ...in transient-transfection ...
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Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2
... FLAG-ZIC2 transfection (Figure 3B). By the transient transfection of the expression vector of FGFR3-IIIb splice variant of human FGFR3 gene, we also found that the FGFR3 up-regulation increased the ...
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N-Terminal Gag Domain Required for Foamy Virus Particle Assembly and Export
... Analysis of cellular protein expression, egress of particulate viral material, and infectivity after transient transfection of 293T cells with the pcHSRV2 point mutants.. In an immunoblo[r] ...
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Functional Characterization of the Human Immunodeficiency Virus Type 1 Genome by Genetic Footprinting
... during transfection, complementation could occur between trans-acting elements in a transfection experiment ...of transient transfection was conducted, cotransfecting the mutant library with a ...
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Rhesus Cytomegalovirus Contains Functional Homologues of US2, US3, US6, and US11
... For transient-transfection experiments, cells were cotransfected with a plasmid encoding green fluo- rescent protein (GFP) at a ratio of 8:1 to distinguish between transiently transfected cells and ...
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Open reading frame 26 of human herpesvirus 8 encodes a tetradecanoyl phorbol acetate- and butyrate-inducible 32-kilodalton protein expressed in a body cavity-based lymphoma cell line.
... BCBL1 cells were found to express HHV8 ORF26 at high levels upon being induced by TPA or sodium butyrate. Immu- nofluorescence analysis of TPA- or sodium butyrate-induced cells revealed a fivefold increase in the number ...
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Zinc Finger and X-Linked Factor (ZFX) Binds to Human SET Transcript 2 Promoter and Transactivates SET Expression
... Transient transfection with plasmid DNA or siRNAs was carried out in HEK 293 and HeLa cells using the Effectene transfection kit (Qiagen, Valencia, CA, USA) and the HiPerFect transfection kit ...
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Establishment of transient and stable transfection systems for Babesia ovata
... for transient transfection, we intro- duced 50 μg of plasmid constructs with transfection conditions of low voltage/high capacitance ...the transfection with plasmid- preloaded ...for ...
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Development of a tetracycline inducible lentiviral vector with an instant regulatory system : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science (MSc) in Biochemistry at Massey University, Manawatu, New Zealand
... This unexpected result could be due to several factors. For instance, the infection efficiency was too low to give a reasonable amount of gene expression. To solve this problem, viral infection was attempted in 24-well ...
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Screening for differentially expressed miRNAs in Aedes albopictus (Diptera: Culicidae) exposed to DENV 2 and their effect on replication of DENV 2 in C6/36 cells
... Results: Six small libraries of Ae. albopictus midgut RNAs were constructed, three of which from mosquitoes that were infected with DENV-2 after feeding on infected blood, and another three that remained uninfected with ...
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An N terminal domain helical motif of Prototype Foamy Virus Gag with dual functions essential for particle egress and viral infectivity
... Infectivity of viral supernatants produced was deter- mined by a flow cytometric based EGFP marker gene transfer assay as described previously [24]. HT1080 cells were seeded at the density of 2 × 10 4 cells/well in 12- ...
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Regulation of major histocompatibility complex class II gene expression in trophoblast cells
... in transient trans- fection assays of HeLa cells, and that the sequences of the CIITA PIV between the GAS and IRE were required for transcriptional ...in transient transfection assays of trophoblast ...
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Inhibition of DNA methyltransferase 1 increases nuclear receptor subfamily 4 group A member 1 expression and decreases blood glucose in type 2 diabetes
... Our previous genome-wide association studies showed that DNA methyltransferase 1 (DNMT1) is associated with increased susceptibility to type 2 diabetes (T2D) in Han Chinese individuals. Here, we aimed to further evaluate ...
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Studies on a new human herpesvirus, Kaposi's sarcoma-associated herpesvirus
... This suggests that in transient transfection systems using 293HEK cells the recombinant expression of KSHV 0RF47/gL only possible at levels undetectable by western blot procedures. 4.5.1[r] ...
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The RR1 gene of herpes simplex virus type 1 is uniquely trans activated by ICP0 during infection.
... The presence of an octamer/TAATGARAT sequence in the 5' regulatory region of the HSV-1 and HSV-2 genes, which is responsive to trans activation by VP16 in transient transfection assays, [r] ...
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Identification of Six Autographa californica Multicapsid Nucleopolyhedrovirus Early Genes That Mediate Nuclear Localization of G-Actin
... in transient transfection experiments, we identified six AcMNPV early genes that mediate nuclear localization of G-actin in TN-368 cells: ie-1, pe38, he65, Ac004, Ac102, and ...
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A mutation in a functional Sp1 binding site of the telomerase RNA gene (hTERC) promoter in a patient with Paroxysmal Nocturnal Haemoglobinuria
... Sp1.2). Transient transfection of hTERC promoter-luci- ferase constructs confirmed that this site is a functional regulator of hTERC core promoter activity and that intro- duction of the patient mutation at ...
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Packaging Cells Based on Inducible Gene Amplification for the Production of Adeno-Associated Virus Vectors
... by transfection of HeLa cells with plasmids pUHG17-1 encoding rtTA and pBSbsr2 as a blasticidin-S re- sistance ...by transient transfection of ptetO-luc and measurement of luciferase expression in ...
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