Transposon Mutagenesis
Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations
... Tn4001 transposon is effective in generating inser- tional mutations in a number of different Mycoplasma species [16,18,19,34] but even using our optimised transformation conditions, we were unable to demon- ...
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Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans
... heterologous transposon and machine learning (ML) analy- sis method, to predict the degree to which all of the open reading frames are re- quired for growth under standard laboratory ...
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Employing transposon mutagenesis to investigate foot-and-mouth disease virus replication
... single transposon inser- tion was identified within 3D pol (3D450), which replicated efficiently in both BHK-21 and MDBK ...a transposon mutagenesis study of poliovirus (Teterina et ...
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Construction and Transposon Mutagenesis inEscherichia coli of a Full-Length Infectious Clone of Pseudorabies Virus, an Alphaherpesvirus
... viral transposon mu- tants. Transposon mutagenesis offers a fast procedure for the isolation of random insertion mutations in ...insertion mutagenesis of a herpesvirus was first re- ported in ...
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Inferring the Minimal Genome of Mesoplasma florum by Comparative Genomics and Transposon Mutagenesis
... media. Transposon mutagenesis of both strains in a set of different media would presumably lead to the identification of many environment-specific essential ...
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Transposon Mutagenesis of the Zika Virus Genome Highlights Regions Essential for RNA Replication and Restricted for Immune Evasion
... Insertion sites in the structural proteins C, prM, and E were enriched after passaging of the mutant viruses. Specifically, the E protein, which sits on the virus surface, was highly tolerant of transposon ...
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The Influenza B Virus Hemagglutinin Head Domain Is Less Tolerant to Transposon Mutagenesis than That of the Influenza A Virus
... a transposon screen to compare the mu- tational tolerance of the currently circulating influenza A virus HAs (H1 and H3 sub- types) and influenza B virus HAs (B/Victoria87 and B/Yamagata88 antigenic ...tolerated ...
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Identification of Essential Genes in the Human Fungal Pathogen Aspergillus fumigatus by Transposon Mutagenesis
... for transposon mutagenesis were obtained using the following pro- ...Insertional mutagenesis of strain CEA17 has led to the isolation of spore color mutants CEA82 and CEA85 ...
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The utility of transposon mutagenesis for cancer studies in the era of genome editing
... employing transposon mutagenesis In vivo transposon mutagenesis requires up to four transgenic alleles to accelerate tumorigenesis in a tissue- specific manner in a sensitizing ...SB ...
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Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
... ABSTRACT Transposon mutagenesis is a widely applied and powerful genetic tool for the discovery of genes associated with selected ...Himar transposon mutagenesis system for generating ...
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Hermes Transposon Mutagenesis Shows [URE3] Prion Pathology Prevented by a Ubiquitin-Targeting Protein: Evidence for Carbon/Nitrogen Assimilation Cross Talk and a Second Function for Ure2p in Saccharomyces cerevisiae
... DNA transposon from the house fly re- lated to the Drosophila hobo element and, distantly, to the Ac transposon of maize (Warren et ...a transposon in ...genome-wide mutagenesis and insertion ...
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TnphoA and Its Use in Transposon Mutagenesis
... the transposon Tn5, which contained a kanamycin resistance gene flanked by two IS50 ...the transposon must be inserted into the correct orientation and the same reading frame as the gene has been inserted ...
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Transposon mutagenesis with coat color genotyping identifies an essential role for Skor2 in sonic hedgehog signaling and cerebellum development
... Beauty transposon, which expresses a tyrosinase minigene that confers four particularly useful features (Lu et ...the transposon with a functional copy of tyrosinase will lead to dose-dependent and ...
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Case-oriented pathways analysis in pancreatic adenocarcinoma using data from a sleeping beauty transposon mutagenesis screen
... by transposon insertion more than expected by chance ...by transposon insertions within/near genes in those ...by transposon insertion in the same tumors more often than would be expected by ...
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Transposon Mutagenesis of the Mouse Germline
... Analysis of the sequence flanking each insertion site did reveal a tendency for SB transposase to select TA dinucleotides that occurred within AT-rich regions (Fig- ure 5). Although this difference was statistically ...
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Use of pulsed field gel electrophoresis and transposon mutagenesis to estimate the minimal number of genes required for motility in Caulobacter crescentus.
... et al. DNA fragments are separated by PFGE and identified by the presence of an insertion or deletion mutation or by hybridization to radioactive probe DNA. If the genetic[r] ...
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Dissection of Filamentous Growth by Transposon Mutagenesis in Saccharomyces cerevisiae
... “Approximate position (within 25 bp) of Tn3::LEU2 insertions in the promoter region (Prom) or the protein coding region (ORF) are given relative to the translational start[r] ...
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Employing transposon mutagenesis to investigate foot and mouth disease virus replication
... Figure S1. Replication of the transposon-mutated replicon library. BHK-21 cells seeded into 12-well plates were allowed to adhere for 16 hours before transfection with transposon-mutated replicon library ...
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Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis
... The statistical analysis that we employed in this study was a hidden Markov model that takes advantage of both the identified sequence preference for insertion and the high level of saturation. Specifically, the HMM uses ...
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Identification of pathogenicity factors in the xylem-invading pathogen Xanthomonas albilineans by Transposon Mutagenesis
... 6:00-6:30 Shuttle picks up participants from The Fawcett Center and transports to The Blackwell Inn 7:00-9:00 Conference Banquet and Best Short Talk and Poster Awards (at The Blackwell I[r] ...
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