Two-dimensional difference in-gel electrophoresis
Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients
... In the preparative gel stained with colloidal coomassie, all the 26 differentially expressed spots could not be visualized, probably because of low abundance. Of the fif- teen spots digested and analysed by mass ...
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Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting
... 2D gel images using DeCyder software enabled detection of 150-200 spot features in both Trichinella proteomes over the pI range of ...2-DE gel regions containing chosen species-specific protein spots ...
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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis
... the two detergents – LPC and MEGA 10 working in ...the two alternate detergents as opposed to a result of the difference in CHAPS concentration during extraction since this protein was not recovered ...
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Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus
... 2-fold difference in abun- dance (ratio of the mean of the normalized spot volume of the TGEV-infected samples versus mock-infected samples) were considered as up-regulated (ratio > 2) or down- regulated (ratio ...
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Proteome changes of lungs artificially infected with H PRRSV and N PRRSV by two dimensional fluorescence difference gel electrophoresis
... non-redundant (NCBInr) database (EST_chordata chordata_20081008 (87827958 sequences; 17755145374 residues)), taxonomy of other mammalia (23009496 sequences); tryptic peptides with max one missed cleav- age site; fixed ...
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Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach
... (2D-Difference Gel Electrophoresis) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometry to identify proteins that may serve as brain tumor antigens ...
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Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
... used two-dimensional difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF mass spectrometry (MS), to investigate secreted proteins in Nipponbare embryo cell suspension ...
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Proteomics in India: the clinical aspect
... 2DE: two–dimensional electrophoresis; 2D DIGE: two-dimensional difference gel electrophoresis; ACTREC: Advanced Centre for Treatment, Research and Education in ...
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A unique protein profile of peripheral neutrophils from COPD patients does not reflect cytokine-induced protein profiles of neutrophils in vitro
... Second dimensional SDS-PAGE was performed on hand-cast 12% SDS-PAGE gels using low fluorescence glass ...plates. Electrophoresis was carried out at 0.2 watts/gel for 2 hours followed by 1 ...
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Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease
... DIGE: Two dimensional fluorescent difference in-gel electrophoresis; 2D PAGE: Two dimensional polyacrylamide gel electrophoresis; ANOVA: Analysis of variance ...
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Clustering of Clinical Strains ofHelicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis
... tein spots to polyvinylidene difluoride (PVDF) membranes was performed. The membranes were air dried, rewetted in meth- anol, rinsed in sterile water, and blocked for 20 min in 0.5% Tween 20. Pooled sera from 10 H. ...
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Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis
... Albumin and immunoglobulin G constitute about 65% and 15% of total protein concentrations in the plasma respectively[6]. The identification of potential less abun- dant disease biomarkers may be complicated or masked by ...
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Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm
... determine the protein contents of coelomic fluid obtained from Eisenia foetida earthworm. The results of our study demonstrated a number of proteins with pH ranging from 3-6, and molecular weight of 10-150 kDa (mostly ...
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Preterm intraamniotic infection and inflammation: Search for protein biomarkers via proteomics approach
... The two-dimensional gel electrophoresis (2-DE) method separates proteins using two different physiochemical ...polyacrylamide gel electrophoresis (SDS_PAGE) [130]. ...
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Comparison of staining methods for two dimensional electrophoresis gel resolved with Puntius javanicus liver proteome
... slab gel and electrophoresis was performed at 16mA for 30 minutes and 24 mA for 2 hours and 30 ...the electrophoresis was completed, the gel was washed with water for 10 minutes and repeated ...
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Two Dimensional Electrophoresis pdf
... of two steps: (1) the separation of protein mix- tures by 2D electrophoresis, and (2) the identi R cation of the separated proteins by analytical techniques, such as mass spectrometry and amino acid ...
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One dimensional Polyacrylamide Gel Electrophoresis pdf
... Electrophoresis is based on the differential migration of electrically charged particles in an electric R eld. As such, the method is applicable only to ionic or ionogenic materials, i.e. substances convertible to ...
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Second-step transfer of bacteriophage T5 DNA: purification and characterization of the T5 gene A2 protein.
... We used uninfected cells, T5amH16d-infected cells, T5amH231-infected cells, and T5bi-infected cells as samples for two-dimensional gel electrophoresis.. We detected four spots on the gel[r] ...
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Means and method of detection in chemical separation procedures
... A method of detection of certain components of an ionic mixture separated or gel electrophoresis comprising: impregnating a two-dimensional medium for separating components of the mixtur[r] ...
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Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach
... Aliquots of total Xenopus egg extract containing 50 µg of protein were dissolved in rehydration buffers formulated according to the L9 orthogonal Taguchi array shown in Table 1 and then separated by 2DE. The resulting 2D ...
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