Two-dimensional mass spectrometry
Application of tandem two dimensional mass spectrometry for top down deep sequencing of calmodulin
... Abstract. Two-dimensional mass spectrometry (2DMS) involves simultaneous acquisition of the fragmentation patterns of all the analytes in a mixture by correlating their precursor and frag- ...
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Phase relationships in two dimensional mass spectrometry
... one-dimensional mass spectra show improved signal-to-noise ratio (up to a factor of ...mode mass spectra was achieved with the development of the Autophaser software by Kilgour et ...mode mass ...
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Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two dimensional gel electrophoresis and mass spectrometry
... Peptide mass spectra were obtained on an Applied Biosystem Sciex 4800 MALDI-TOF⁄TOF mass spectrom- eter (Applied Biosystems, ...a mass range from 800 to 4000 with a focus mass of ...
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Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry
... Raw files were converted to MGF files using MSConvert from the ProteoWizard open-source project [31] at [http://proteowizard.sourceforge.net/downloads.shtml]. The first pass search of the MS/MS spectra was against the ...
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Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry
... from two cleaved, disulfide-linked triticin precursors ...least two copies each of globulin genes Glo3A, 3B and 3D were reported for hexaploid wheat and mapped to chromosome 4; at least one of the protein ...
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Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry
... uals was compared (table 2). 8 of these spots were identi- fied as different fragments of albumin and all these were up-regulated in allergic individuals (1.8- to 2.6-fold). Wnt-2B (splice isoform 1), transthyretin and ...
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Proteomic profiling of the rat hypothalamus
... chloroform/methanol. Two-dimensional (2-D) gels of hypothalamic extracts from four-month-old rats were analyzed; the protein spots were digested and identified by using tandem mass ...
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Analysis of three dimensional cell cultures using mass spectrometry imaging
... the mass, however expression changes may also be contributing to ...using two separate statistical methods and 10 highly differing species were detected using both tests, which may be indicative of the ...
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Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo
... ducted two proteomic studies using immortalized human HTR-8/SVneo trophoblastic ...via two-dimensional gel electrophoresis (2DE), and protein spots were identified via tandem mass ...
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Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences
... In this work, we propose to directly use raw genome sequences for identifying proteins separated by 2-DE and analyzed by mass spectrometry. The method is demon- strated for proteomic analysis of Klebsiella ...
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A Three-Dimensional Microfluidic Device Coupled with Paper Spray Ionization Mass Spectrometry for the Detection of Triazines.
... a two-dimensional µPAD can detect triazines using PS-MS and a piece of filter paper patterned with ...the two-dimensional µPAD can be coupled with PS-MS for detection, the device fails to ...
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Proteomic analysis of prolactinoma cells by immuno-laser capture microdissection combined with online two-dimensional nano-scale liquid chromatography/mass spectrometry
... In a typical shotgun proteomics experiment, a com- plex protein sample is digested into peptides followed by separation by 2D-nanoLC/MS, resulting peptides were loaded into a mass spectrometer for fragmentation ...
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The Detection of THCA Using 2 Dimensional Gas Chromatography Tandem Mass Spectrometry in Human Fingernail Clippings: Method Validation and Comparison with Head Hair
... The specimens were analyzed using an Agilent Tech- nologies (Santa Clara, CA, USA) 7890A GC equipped with a LTM Series II System, Agilent Dean’s Switch and 7693 Autosampler. Samples were injected in the splitless mode. ...
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Complementary Mass Spectrometry Methods for Characterizing Protein Folding, Structure, and Dynamics
... (14). Two-dimensional hydrodynamic focusing causes the compression of a central liquid stream into a thin filament by a coaxial sheath flow, ensuring submillisecond diffusive mixing ...
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A simple and reliable protocol for mouse serum proteome profiling studies by use of two-dimensional electrophoresis and MALDI TOF/TOF mass spectrometry
... Tryptic peptides were spotted directly onto a 600 μm/384 well AnchorChip™ sample target (Bruker Daltonics). The matrix CHCA was saturated in 97% Acetone/0.1% TFA solution; DHB matrix was solved in 30% ACN/0.1% TFA ...
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Comprehensive two-dimensional gas chromatography–mass spectrometry combined with multivariate data analysis for pattern recognition in Ecuadorian spirits
... sealed two-stage cryogenic modulator that provided cold (T = − 196 °C) and hot (T = 250 °C) jets that were controlled by sole- noid valves (ASCO, Florham Park, NJ—USA) and a 8-bit Duemilanove microcontroller board ...
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Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv.oryza (Xoo)
... used two-dimensional difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF mass spectrometry (MS), to investigate secreted proteins in Nipponbare embryo cell suspension culture ...
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Salivary protein profiling in type I diabetes using two-dimensional electrophoresis and mass spectrometry
... Until recently, the study of salivary protein modification occurring in type 1 diabetes was mainly based on techniques targeting specific proteins. For instance, using enzymatic assays, salivary concentration of ...
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Comprehensive analysis of the mouse renal cortex using two-dimensional HPLC – tandem mass spectrometry
... different mass spec- trometry runs, these results suggest that the variation in reproducibility in protein identifications can mostly be accounted for by the mass spectrometer, and not by vari- ability ...
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Proteins isolated with TRIzol are compatible with two dimensional electrophoresis and mass spectrometry analyses
... average mass of horse myoglobin without the N- terminal methionine because of cotranslational ...considerable mass modifications in myoglobin, although we cannot exclude mass changes that occur at a ...
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