Upstream Open Reading Frame (uORF)
Reselection of a Genomic Upstream Open Reading Frame in Mouse Hepatitis Coronavirus 5′-Untranslated-Region Mutants
... AUG-initiated upstream open reading frame (uORF) encoding a potential polypeptide of 3 to 13 amino acids (aa) is found within the 5= untranslated region (UTR) of >75% of coronavirus genomes ...
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Abundant Early Expression of gpUL4 from a Human Cytomegalovirus Mutant Lacking a Repressive Upstream Open Reading Frame
... The human cytomegalovirus UL4 gene encodes a 48-kDa glycoprotein, expression of which is repressed at the translational level by a short upstream open reading frame (uORF2) within the UL4 ...
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Translational Effects of Mutations and Polymorphisms in a Repressive Upstream Open Reading Frame of the Human Cytomegalovirus UL4 Gene
... 22-codon upstream open reading frame (uORF2), the peptide product of which represses downstream translation by blocking translation termination at its own stop codon and by causing ribosomes ...
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Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
... To better understand the effects of changes in temperature on start site selection, we identified a set of 1367 uORFs that show evidence of translation in our yeast strain at one or more temperatures. To this end, we ...
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Translational inhibition by a human cytomegalovirus upstream open reading frame despite inefficient utilization of its AUG codon.
... codon is suboptimal (ACCaugG is optimal, with the under- lined A at 2 3 and G at 1 4 being the most influential bases [11]) or if the AUG codon is very close to the cap site (14, 22), the 40S subunits may bypass the AUG ...
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Translational Repression of the RpoS Antiadapter IraD by CsrA Is Mediated via Translational Coupling to a Short Upstream Open Reading Frame
... far upstream of the iraD ribosome binding ...Three open reading frames (ORFs), all of which are translated, were identi- fied in the iraD leader ...
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Efficiency of reinitiation of translation on human immunodeficiency virus type 1 mRNAs is determined by the length of the upstream open reading frame and by intercistronic distance.
... an upstream ORF of 84 nucleotides and should be entirely abrogated by an ORF longer than 165 nucle- otides (predicted by extrapolating the linear range of the curve in ...
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Translational inhibition mediated by a short upstream open reading frame in the human cytomegalovirus gpUL4 (gp48) transcript.
... To clarify which additional gp48 leader sequences were required elements of the inhibitory signal, we constructed plasmids that express transcripts containing wild-type or mutant gp48 le[r] ...
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Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene.
... We have found that, as in PrV, a portion of an upstream open reading frame exhibiting extensive nucleotide and amino acid sequence similarity 58.9% similarity over 175 amino acids to the[r] ...
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A Stem-Loop Structure in Potato Leafroll Virus Open Reading Frame 5 (ORF5) Is Essential for Readthrough Translation of the Coat Protein ORF Stop Codon 700 Bases Upstream
... 27, 52–54). The readthrough protein of PLRV plays key roles in several processes, including virus movement, accumulation, and symptom development in plant hosts, as well as in the virus life cycle within aphids. In this ...
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Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein
... different reading frames and the only known promoter driving the transcription of these constructs lies upstream of the ORF 2 coding ...ribosomal frame- shifting, internal initiation, or ...
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Murine Gammaherpesvirus 68 Open Reading Frame 11 Encodes a Nonessential Virion Component
... size of the band was consistent with the predicted size of the ORF11 gene product (42.5 kDa). We also tagged ORF11 in situ with CFP to give a predicted 70-kDa fusion protein. To do this, we used PCR to generate 1.2-kb ...
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Human Cytomegalovirus UL131 Open Reading Frame Is Required for Epithelial Cell Tropism
... just upstream of UL131, and 176207, within UL130 (number- ing according to reference ...end upstream of the UL131 coding region has the potential to encode polypeptides encoded by all three ORFs, whereas ...
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Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization.
... (CAT) open reading frame generated GBV-CAT fusion proteins in ...immediately upstream of the putative signal sequence for the GBV E1 envelope ...
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Quantification and Analysis of Thymidine Kinase Expression from Acyclovir-Resistant G-String Insertion and Deletion Mutants in Herpes Simplex Virus-Infected Cells
... ⫹1 frame immediately upstream of the G string (Stop ⫹ 1a), in the 0 frame after the sixth G in the G string (Stop0b), or three codons downstream of the G string ...N-terminal reading ...
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Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.
... Transformed cell lines were established from a plate containing >50 foci induced by the indicated viral DNAs.. Cells were then grown in 0.3% agarose in Dulbecco modified Eagle medium-10%[r] ...
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Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation.
... Previous studies have identified a region of the herpesvirus saimiri genome required for immortalization of marmoset lymphocytes in vitro and for oncogenicity in vivo; this region is not[r] ...
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Deletion of the VP16 open reading frame of herpes simplex virus type 1.
... Deletion mutant virions propagated in this cell line contained wild-type, cell-derived VP16 protein that was recruited during virion assembly and was functional for immediate-early gene [r] ...
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Bovine herpesvirus 1 U(s) open reading frame 4 encodes a glycoproteoglycan.
... To determine whether the 65-kDa protein released from the US ORF4 proteoglycan carries carbohydrates, [35S]methionine-cysteine-labeled proteins from the supernatants of cells infected wi[r] ...
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Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... In this series of experiments UL32 fusion protein expressed by recombinant baculovirus in insect cells or R7032-infected RS cell lysates were immunoprecipitated with UL32 polyclonal anti[r] ...
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