Ussing chamber
The Ussing chamber system for measuring intestinal permeability in health and disease
... Optimisation of dissection techniques for both mouse and human tissue is important for viability. In this study, experiments ran for a total of ninety minutes, with for- skolin addition at sixty minutes. Lengthier ...
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Active osmoregulatory ion uptake across the pleopods of the isopod Idotea baltica (Pallas): electrophysiological measurements on isolated split endo and exopodites mounted in a micro ussing chamber
... however, we found neither structural nor electrophysiological differences between the two epithelial sides. The absence of physiological differences (Fig. 3) between the sediment-facing and the pleon-facing half-lamellae ...
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Calcium induced chloride secretion is decreased by Resveratrol in ileal porcine tissue
... Fig. 2 Exemplary time course of short circuit currents (I sc ) as measured in Ussing chamber studies. The respective chambers were started with chloride containing or chloride free buffer solutions ...
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Activation of the calcium-sensing receptor attenuates TRPV6-dependent intestinal calcium absorption
... an Ussing chamber (EM-CYS-4 system with P2400 chambers and P2407B sliders, Physiologic Instruments) and incubated with 4 ml Ringer’s solu- tion consisting of 115 mM NaCl, ...
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CPT-cGMP Is A New Ligand of Epithelial Sodium Channels
... using Ussing chamber and voltage clamp techniques showed that 8-pCPT-cGMP increased the amiloride-sensitive short-circuit current across H441 cell monolayers and also increased heterologously expressed ...
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Abnormal vitamin D metabolism, intestinal calcium transport, and bone calcium status in the spontaneously hypertensive rat compared with its genetic control
... Abnormalities of intestinal calcium absorption and the vitamin D axis in the spontaneously hypertensive rat (SHR) are controversial. The present report documents a reduction in circulating 1,25-dihydroxyvitamin D3 ...
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Structural alterations of the intestinal epithelial barrier in Parkinson’s disease
... gastrointestinal biopsies either paracellularly or transcel- lularly over a 3 hour period [34]. Using this approach, we showed that there were no significant differences in para- and transcellular permeabilities between ...
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Hydrogen peroxide stimulates rat colonic prostaglandin production and alters electrolyte transport
... 3 depicts the effect of SOD on the AIsc response to ROMs created by the X/XO reaction in the serosal bathing solution of rat colon mounted in the Ussing chamber.. The response of the tis[r] ...
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Virus protein transport across the gut epithelium of Spodoptera frugiperda (Lepidoptera: Noctuidae)
... an Ussing chamber to examine the transport efficiencies and mechanisms involved with the movement of a virus-derived coat protein and bovine serum albumin across the gut of Spodoptera ...
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Determinants of mild clinical symptoms in cystic fibrosis patients Residual chloride secretion measured in rectal biopsies in relation to the genotype
... Previous Ussing chamber measurements of secretagogue-provoked changes in short circuit current in rectal suction biopsies of cystic fibrosis (CF) patients showed that in a minority of patients chloride ...
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Active absorption of Na+ and Cl across the gill epithelium of the shore crab Carcinus maenas: voltage clamp and ion flux studies
... needle was pressed through the ventral side of the body wall), the carapace was lifted and the three posterior gills were removed. Single gill lamellae were isolated and split according to the method described by Schwarz ...
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Vapour Explosion Chamber
... the chamber may be increased by heating the liquid in ...the chamber until the chamber is full or contains a predetermined quantity of ...the chamber under high ...the chamber through ...
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Axial Flow Characteristics within a Screw Compressor
... The same dual beam LDV-system as that of [2,3] was used to obtain the angle-resolved axial mean and the turbulence characteristics of the flow inside the working chamber. Measurements were performed for both the ...
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Transport properties of cultured branchial epithelia from freshwater rainbow trout: a novel preparation with mitochondria rich cells
... Comparisons between measured and predicted Ussing flux ratios for Na+ and Cl− across cultured DSI epithelia under symmetrical culture medium on both surfaces and asymmetrical conditions [r] ...
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A new plant chamber facility, PLUS, coupled to the atmosphere simulation chamber SAPHIR
... sured at the PLUS inlet and outlet by cavity ring down spectroscopy (CRDS, Picarro, model G2301). Measurements for the inlet and outlet are obtained by switching via a valve system between both measurement ports. This ...
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Changes in Anterior Segment Parameters after Penetrating Keratoplasty for Keratoconus
... In conclusion; due to a shallower ACD, and a more flattened cornea, hyperopic shift (mean spherical equivalent; +4.23 ±3.44 diopters) was seen 13 months after PK surgery in our study. However, flat corneal curvature may ...
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Neutrophil migration across a cultured intestinal epithelium Dependence on a CD11b/CD18 mediated event and enhanced efficiency in physiological direction
... We have previously shown that FMLP induced mucosal-to-serosal transmigration across 2-cm2 monolayers subsequently mounted in Ussing-type chambers results in a fall in transepithelial res[r] ...
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The Role of Intestinal Epithelial Cell CD23 in Food Allergy
... the Ussing chambers, had milk specific IgE which was able to cause some mast cell degranulation that was not profound enough to produce the expected changes in Isc and ...
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The Geographic Embedding of Online Echo Chambers: Evidence from the Brexit Campaign
... We approach H5a, which hypothesizes that echo-chamber communication is associated with geographic proximity in the Leave and Remain campaigns (H5a and H5b, respectively), by comparing the density distribution ...
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Restricting the nonlinearity parameter in soil greenhouse gas flux calculation for more reliable flux estimates
... the fits and does not allow the absolute value of the nonlinear estimate to deviate by more than the g-factor (default is 4) from the absolute value of the linear estimate. Although this mixed FCS has been used in some ...
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