β 1-4 glucosidase
SCREENING AND OPTIMIZATION OF β-GLUCOSIDASE PRODUCTION BY Candida sp. JK9/1
... of β-glucosidase production from Candida sp. JK9/1, effect of biophysical and biochemical factors on β- glucosidase production were ...on β-glucosidase production, Candida ...
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Direct cadaverine production from cellobiose using β-glucosidase displaying Escherichia coli
... Table 1 shows the strains and plasmids used in this study. E. coli JCM20137 was used as the host strain. This bacter- ium, and its derivative possessing a plasmid, were grown in Luria-Bertani medium (10 g/l ...
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Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance
... identified β-glucosidase of GH3, Bgl3A, from Talaromyces leycettanus JCM12802, was overexpressed in yeast strain Pichia pastoris GS115, yielding a crude enzyme activity of 6000 U/ml in a 3 L fermentation ...
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Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
... (1) Purification by Ni‑charged resin (BglucLEH and BglucH) The recombinant proteins (BglucLEH and BglucH) were purified with Ni-NTA resin because both the BglucLEH and BglucH contain His tag. Purification ...
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Non–pseudogene derived complex acid β glucosidase mutations causing mild type 1 and severe type 2 Gaucher disease
... Immunoblotting. Immunoelectroblotting using a polyclonal anti–human acid β-glucosidase antibody (49, 50) was per- formed as described (14, 19). In brief, Sf9 cells infected with pure recombinant virus were ...
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Characterization and application of recombinant β-glucosidase (BglH) from Bacillus licheniformis KCTC 1918
... Abstract β-Glucosidase (β-1,4-d-glucoside glucohydrolase: ...of β-glucosidic bonds between saccharides and aryl or alkyl ...ing β -glucosidase from Bacillus licheniformis KCTC ...
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Production of a high-efficiency cellulase complex via β-glucosidase engineering in Penicillium oxalicum
... release was measured and the absorbance was read at 405 nm. The glucose level was measured with the Biosen- sor. One enzyme activity unit represents the amount of enzyme required to either produce one µmol glucose, or ...
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Purification and characterization of a novel ginsenoside Rc-hydrolyzing β-glucosidase from Armillaria mellea mycelia
... at 4 °C, then homog- enized with an Omni mixer homogenizer (Omni Interna- tional, Kennesaw, GA, USA) for 1 min at 4 ...at 4 °C, the pre- cipitate was dissolved in 10 mM sodium acetate buffer ...
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A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
... Results: In this study, a cDNA encoding β-glucosidase was isolated from the buffalo rumen fungus Neocallimastix patriciarum W5 and is named NpaBGS. It has a length of 2,331 bp with an open reading frame ...
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Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose
... The cellobiose was treated with purified BGL, and the degradation was subjected to analysis of thin-layer chro- matography (TLC) and HPLC. The reaction mixture (20 μ L) contained 290 mM cellobiose, and 1 μ g of ...
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Original Article High mobility group box protein 1 downregulates acid β-glucosidase 1 in synovial fibroblasts from patients with rheumatoid arthritis
... Among them, GBA1 was down-regulated by 4 folds. GBA1 is a widely expressed lysosomal enzyme that cleaves glucosylceramide into ceramides. Ceramides are central in the sphin- golipids metabolism and mainly involved ...
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Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase
... Thereafter, the ratio between EBY100/CelA and EBY100/ CBHII was investigated while fixing the amount of EBY100/mini CipA as 1/4 of total cells. EBY100/CelA and EBY100/CBHII secreted similar amounts of ...
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Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar
... a 1 % (v/v) protease inhibitor cocktail (P9599; Sigma-Aldrich, ...DTT, 1 % (v/v) protease inhibitor cocktail, and 50 mM MES–KOH ad- justed to pH ...approximately 1 mL volume with a cen- trifugal ...
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A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins
... The plant cell wall consists of three major components, whose proportions depend on the source of the ligno- cellulosic biomass: cellulose, hemicellulose, and lignin [5]. Nowadays, commercial enzymatic cocktails used for ...
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The putative β-glucosidase BGL3I regulates cellulase induction in Trichoderma reesei
... real β-glucosidase that cleaves β-1-4-bonds in cello-oligo- saccharides into glucose units (Table 1); however, its ability to degrade pNPG and other oligosaccharides was ...
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A β Glucosidase Activity Potentially Involved in Cell Division and Wall Development of Phyllostachys Bamboo Suspension Cells
... NaCl, 1 mM EDTA, pH ...h, 4˚C). After centrifugation (10,000 × g, 10 min, 4˚C), the supernatants collected were further centrifuged (10,000 × g, 10 min, ...min, 4˚C), and were re-suspended to ...
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Binding Mode Study of <i>β</i>-glucosidase B from <i>P. Polymyxca</i> with Cellobiose and Laminaribiose - Volume 4 Number 6 (Dec. 2013) - IJCEA
... Glutamate is important for reducing the energy barrier of the glycosylation step [10]. The glutamate side-chain can adapt its position to an axial O4 and has the ability to recognize galacto-configured substrates due to ...
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A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production
... low β-glucosidase activity, which reduces efficiency in biomass degradation and compromises its industrial application ...secreted β-glucosidase com- prises only about 1 % of the total ...
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Induced Production of Exoglucanase, and β Glucosidase from Fungal Co Culture of T viride and G lucidum
... Figure 3 illustrates that the initial enzyme activity increased with the increase of pH and the peak activity was at pH 6, while further increase in pH showed decreasing trend in enzyme activity. It has been reported ...
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Strictosidine β D Glucosidase (SGD) Gene Study Case in the three Egyptian Cultivars of Catharanthus roseus
... The PCR cycling condition involved initial denaturation at 94°C for 4 min. followed by 35 cycles, template denaturation at 94°C for 1 min., primer annealing at 52°C for 1 min. and primer extension at ...
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