Β-Glucosidase
Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases
... A gene of 1380 bp encoding a β-glucosidase from A. tumefaciens, with the same sequence as that reported in GenBank (EHJ97046.1), was cloned (as described in “Experimental” section) and expressed in E. coli. ...
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Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase
... that β-glucosidase can bind to the yeast surface without any modification, endoglucanase and exoglucanase, but not β-glucosidase, were incorporated as cellulosome ...
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Secretive expression of heterologous β-glucosidase in Zymomonas mobilis
... extracellular β-glucosidase activity was detected in the constructed recombinants, it was still not sufficient to give the effective CBP fermentation ...the β-glucosidase activity is required ...
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Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
... We targeted the β-glucosidase from A. niger (BGL1) using directed evolution to adapt it towards heterolo- gous expression S. cerevisiae. We built a fusion protein by replacing the native BGL1 signal ...
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Sorption to Biochar Impacts β-Glucosidase and Phosphatase Enzyme Activities
... Abstract: Extracellular enzymes catalyze biogeochemical reactions in soil, cycling carbon and nutrients in agricultural systems. Enzymes respond quickly to soil management, including organic amendment inputs, such as ...
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Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation
... 1837, β-glucosidase was found to be widely present in kinds of microorganisms including Aspergillus, bacteria, and Saccharomyces (Kitagawa et ...of β-glucosidase (Sternberg et al. 1977). How- ...
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ENZYME ASSAY, CLONING AND SEQUENCING OF NOVEL β-GLUCOSIDASE GENE FROM ASPERGILLUS NIGER F321 (UNIDENTIFIED NIGERIAN STRAIN)
... of β-glucosidase genes from Aspergillus niger (Meko’o et ...niger β- glucosidase for its heterologous expression in Pichia pastoris using methanol as an ...thermostable β- ...
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Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1
... The biochemical properties of DT-Bgl were determined using the purified recombinant DT-Bgl. When using pNPG as substrate, the purified DT-Bgl exhibited maxi- mum activity at 70 °C and pH 8.5, while its relative activ- ...
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SCREENING AND OPTIMIZATION OF β-GLUCOSIDASE PRODUCTION BY Candida sp. JK9/1
... of β-glucosidase production from Candida ...on β- glucosidase production were ...on β-glucosidase production, Candida ...maximum β-glucosidase activity whereas when ...
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The putative β-glucosidase BGL3I regulates cellulase induction in Trichoderma reesei
... these β-glucosidase genes were upregulated by Xyr1 in the inducing medium (wheat bran and Avicel); however, a β-glucosidase gene named bgl3i (cel3g) was downregulated ...other ...
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“Kinetic Parameters of Purified β-glucosidase from the Seeds of Tamarindus indica” by Shlini P, K R Siddalinga Murthy, India.
... purified β-glucosidase isolated from germinated tamarind ...mins. β-glucosidase enzyme showed a maximum activity at pH pH ...C. β-glucosidase exhibited Km value of 121 µM and ...
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The β-glucosidase secreted by Talaromyces amestolkiae under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass
... For BGL-3* purification, 7-day-old cultures were har- vested and centrifuged at 10,000×g and 4 °C for 20 min. The supernatant was first concentrated by tangential fil- tration and finally concentrated and dialyzed ...
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Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose
... novel β -glucosidase (BGL) gene bgl from ...the β - Glucosidases from thermophile, and was distant from the other glucose-tolerant β ...than β-glucosidases from other ...novel ...
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α Glucosidase and β glucosidase from psychrotrophic strain arthrobacter sp C2 2
... α-glucosidase, β-glucosidase, α-mannosidase and ...and β-glucosidase from the psychrotrophic strain Arthrobacter ...kDa, β-glucosidase – 93 kDa) were determined by gel ...
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Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar
... To pursue our line of enquiry on PtrBGL6, a candidate coniferin BGL from poplar, an antibody was raised using specific peptides of PtrBGL6 (Fig. 2b). The activity of BGL on pNPG was monitored as a general BGL; this ac- ...
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Carbohydrate-Modified Electrode Surfaces Sensitive to β-Glucosidase Enzyme Activity
... Since electrodes modified with either 2 or 3 did not appear to be efficiently recognized by SAB, we decided to try using another enzyme: the retaining -glucosidase from Agrobacterium sp. (Abg). Abg is a ...
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Diversity of marine bacteria producing beta-glucosidase inhibitors
... Glucosidase inhibitors are emerging as important thera- peutic agents since they interfere with crucial metabolic functions in biological systems, and have widespread ap- plications in pharmacology and ...
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The effect of drinking milk containing conjugated linoleic acid on fecal microbiological profile, enzymatic activity, and fecal characteristics in humans
... fecal β-glucuroni- dase, nitroreductase and azoreductase enzyme activities did not change when human subjects consumed 500 ml of low fat milk per day for 30 ...[38], β-glucuroni- dase enzyme activity ...
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Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover
... To evaluate the hydrolysis ability of the cellulase cock- tails of T. reesei Rut C30 or PB-3 on natural cellulosic materials, the crude enzyme from T. reesei Rut C30 (Cel- MGD) or PB-3 (Cel-MGD-BGL) were used to ...
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Methodological interference of biochar in the determination of extracellular enzyme activities in composting samples
... and β-glucosidase activities, respec- tively) exhibited high PNP retention from 30 % (acid phos- phatase determination in PM + B) up to 70 % which is the case of the β -glucosidase ...
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