β-Glucuronidase
Indole bearing thiadiazole analogs: synthesis, β-glucuronidase inhibition and molecular docking study
... In conclusion we have synthesized 22 derivatives of indole based thiadiazole hybrid analogs due to greater biological importance of indole and thiadiazole and evaluated for β-glucuronidase inhibition. All ...
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Protein Engineering of Escherichia coli β-glucuronidase
... Proteins from thermophilic organisms generally show not only greater thermal stability, but also stability towards chemical denaturants and proteolysis (Vieille & Zeikus 2001, Leuschner & Antranikian 1995, ...
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Engineering the glycoside hydrolase β-glucuronidase (β-GUS) to improve a non-native activity for the synthesis of O-glucuronides
... When we first noticed that the glucuronylsynthase had higher than expected -glucuronidase activity, we instinctively assumed that this was caused by wild-type - GUS contamination due to an oversight in the ...
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Applying neutral drift to the directed molecular evolution of a β glucuronidase into a β galactosidase: Two different evolutionary pathways lead to the same variant
... If neutral selection does not provide improved variants then it may still be of value if it identifies those variants more efficiently. This is the central claim made by Gupta and Tawfik [20]. In the case of ...
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Lysosomal β glucuronidase regulates Lyme and rheumatoid arthritis severity
... We thank Mark Sands (Washington University) for providing the pCAGG-β-gluc mammalian expression plasmid that was used to create our transgenic overexpression construct. Paul Allen (Wash- ington University) kindly ...
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β-GLUCURONIDASE MUTANTS OF THE NEMATODE CAENORHABDITIS ELEGANS
... elegans corresponds to the frequency of forward mutation expected for an average gene (BRENNER 1974). Four sorts of genetic crosses were performed to position gus-1[r] ...
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Non contiguous-finished genome sequence and description of Enorma massiliensis gen. nov., sp. nov., a new member of the Family Coriobacteriaceae
... urease, β- galactosidase 6 phosphate, α-arabinosidase, β- glucuronidase, N-acetyl-β-glucosaminidase, glu- tamic acid decarboxylase, α-fucosidase, leucyl gly- cine arylamidase, phenylalanine ...
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Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice
... GUS activity was measured by observing cleavage of the β-glucuronidase substrate 4-methylumbelliferyl β-D- glucuronide (MUG) (Sigma, USA; Jefferson 1987; Gallagher 1992). Samples (100 mg) of mock, ...
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Roberts_unc_0153D_14592.pdf
... By demonstrating that SN-38 plasma levels remain the same in the presence of the bacterial β- glucuronidase inhibitor, Inhibitor 1, it suggests that antitumor efficacy of CPT-11 would also remain intact. ...
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13. Establishing Agrobacterium mediated transient gene expression assay in Nicotiana tabacum
... Preliminary functional characterization of a gene within a short time period is only possible through transient gene expression assays. Transient gene expression assays are simple, cost effective and easy to perform as ...
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Non-contiguous finished genome sequence and description of Kallipyga massiliensis gen. nov., sp. nov., a new member of the family Clostridiales Incertae Sedis XI
... for β- galactosidase, β-galactosidase-6-phosphate, α- arabinosidase, β-glucuronidase, N-acetyl-β- glucosaminidase, glutamic acid decarboxylase, proline arylamidase, leucyl glycine ...
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FEASIBILITY ANALYSIS OF LEAF DISC SAMPLES PRODUCED VIA AGROINFILTRATION FOR PROMOTER TRAPPING STUDIES
... Abstract: Promoter trapping is a method used to isolate and characterize regulatory regions from genomes by elucidating the expression of a promoterless reporter gene flanked by two transposable elements. The ...
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Application of sonication assisted Agrobacterium mediated transformation in Chenopodium rubrum L
... for β-glucuronidase in young seedlings of Chenopodium ...of β-glucuronidase activity in two-days-old and ten-days-old seedlings was carried ...of β-glucuronidase activity between ...
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Ex Vivo Gene Therapy for Lysosomal Storage Disease Using Ipsc-Derived Neural Stem Cells
... Frozen fibroblasts were obtained from a 3 month old female patient (GM02784, Coriell Institute for Medical Research) with no detectable β-glucuronidase activity. Fibroblasts were maintained in DMEM ...
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Nematode-responsive activity of the cauliflower mosaic virus 35S promoter and its subdomains
... gave β-glucuronidase (GUS) activity in the meristematic and cortex regions of the root tips, but in the four lines examined here there was no reproducible activ- ity in roots with this ...
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Non contiguous-finished genome sequence and description of Peptoniphilus obesi sp. nov.
... α-fucosidase, β-galactosidase, glutamic acid de- carboxylase, 6-phospho-β-galactosidase β- glucosidase, β-glucuronidase, N-acetyl-β- glucosaminidase, D-mannose, D-raffinose, ...
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Transcriptional analysis of the recA gene of Streptococcus thermophilus
... for β -glucuronidase activity. As shown in Fig. 4, similar levels of β -glucuronidase activity were observed in all strains grown in the presence or in the absence of mito- mycin C (200 ...
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Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L )
... Transient expression of the gus gene in roots or shoots after bombardment was weak (data not shown), proba- bly because of the high content of phenolics in the Hippophae tissue; phenolics in woody tissue often inhibit ...
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Download Download PDF
... standard β-glucuronidase inhibitor both in terms of binding scores and the residues that interact with the drug and β-glucuronidase ...novel β-glucuronidase inhibitors that are ...
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Materials Discovery by Crystal Growth: Synthesis, Structure Determination, Magnetic, and Optical Properties of Complex Lanthanide Containing Oxides, Oxyhydroxides, and Oxyfluorides
... Because meconium is available in a finite quantity, minimizing sample mass required for analysis is desirable to conserve patient specimens for other analyses. Past methods have used 1 g of meconium and required ...
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