Vaccinia Virus
Expression of the M gene of vesicular stomatitis virus cloned in various vaccinia virus vectors.
... CV-1 cells infected with wild-type vaccinia virus or T7 polymerase recombinant vaccinia virus vTF1-6,2 were transfected by calcium phosphate precipitation with the M genecontaining plasm[r] ...
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Vaccinia Virus Encodes a Novel Inhibitor of Apoptosis That Associates with the Apoptosome
... FIG 1 Creation and characterization of an M1L-expressing MVA virus. (A) Schematic showing insertion of tandem GFP and M1L genes into the del III region of the MVA genome, in which the GFP gene is under the control ...
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Intracellular Localization of Vaccinia Virus Extracellular Enveloped Virus Envelope Proteins Individually Expressed Using a Semliki Forest Virus Replicon
... Localization of EEV envelope proteins during vaccinia virus infection. Models for EEV formation and release imply that EEV envelope proteins must be present in the wrapping mem- branes, derived from the ...
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Preclinical Studies of a Modified Vaccinia Virus Ankara-Based HIV Candidate Vaccine: Antigen Presentation and Antiviral Effect
... immunodeficiency virus (HIV) vaccine candidates are currently under evaluation in preclinical and clinical ...Modified vaccinia virus Ankara (MVA) vectors have excellent safety and immunogenicity ...
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Vaccinia virus morphogenesis is blocked by a temperature-sensitive mutation in the I7 gene that encodes a virion component.
... To examine the expression of the 17 gene product during vaccinia virus infection of cultured cells, BSC40 monolayers were infected synchronously with wild-type vaccinia virus and harvest[r] ...
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Variation in vaccinia virus
... of vaccinia virus occurs in mouse brain after intracerebral inoculation, 10^ pfu of the CL strain were inoculated intracerebrally into four teen mice and the brains reaped aseptically 3 - 1 0 minutes ...
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System of Double Infection Between Vaccinia Virus and Mengovirus
... The detection of vaccinia virus-like proteins by disc-gel electrophoresis and the synthesis of DNA in the cytoplasm of double-infected cells suggest that at least some vaccinia virus mes[r] ...
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Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins
... by vaccinia virus (VACV) was described more than 30 years ago (1–3), and recombinant vectors have been extensively deployed since then for immunology and infectious disease research (4, ...defective ...
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In vitro transcription of a cloned vaccinia virus gene by a soluble extract prepared from vaccinia virus-infected HeLa cells.
... Uninfected and vaccinia virus-infected HeLa cell extracts were assayed for transcription of Sall-digested pAG4 with or without supplementation with purified vaccinia virus RNA polymerase[r] ...
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Vaccinia virus gene encoding a component of the viral early transcription factor.
... The latter factor, the vaccinia virus early transcription factor VETF, binds specifically to vaccinia virus early promoters and has an associated DNA-dependent ATPase activity 5.. The AT[r] ...
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Protection of mice and swine from pseudorabies virus conferred by vaccinia virus-based recombinants.
... The resulting plasmid, pPR24, with glll under the control of the B13R promoter, was transfected into vaccinia virus-infected cells to generate vaccinia virus recombinants vP604, vP644, v[r] ...
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Altered Plaque Formation by Recombinant Vaccinia Virus Expressing Simian Immunodeficiency Virus Nef
... altered plaques. Two observations are relevant to residue 211. First, the leucine at residue 211 (the corresponding position in HIV-1 Nef is residue 181) is highly conserved in HIV and SIV Nef and is located in one of ...
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Vaccinia virus-specific human CD4+ cytotoxic T-lymphocyte clones.
... b Clones established by UV-inactivated vaccinia virus stimulation of PBL from a healthy donor were tested for their ability to lyse uninfected or vaccinia virus-infected autologous B-LCL[r] ...
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Activation of the human immunodeficiency virus type 1 long terminal repeat by vaccinia virus.
... To investigate whether vaccinia virus induces HIV LTR-directed gene expression, transient expression assays in Jurkat cells persistently infected with vaccinia virus Jvac using plasmid D[r] ...
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Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodalton fusion protein.
... Because we have specific monoclonal antibodies MAbs that react with the 14-kDa protein of vaccinia virus and rabbit poxvirus and because several vaccinia virus genes such as RNA polymera[r] ...
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Vaccinia Virus Infection Disarms the Mitochondrion-Mediated Pathway of the Apoptotic Cascade by Modulating the Permeability Transition Pore
... that vaccinia virus would encode a mechanism to directly modulate the mitochondrial apoptotic ...of vaccinia virus. In addition, vaccinia virus-infected cells subjected to the ...
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Characterization of a Newly Identified 35-Amino-Acid Component of the Vaccinia Virus Entry/Fusion Complex Conserved in All Chordopoxviruses
... the vaccinia virus (VACV) genome was limited to open reading frames (ORFs) of at least 65 amino ...in virus morphogenesis, though O3-deficient virus had low ...of virus entry, as cores ...
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Vaccinia Virus F9 Virion Membrane Protein Is Required for Entry but Not Virus Assembly, in Contrast to the Related L1 Protein
... Vaccinia virus (VACV) is a member of the Poxviridae, a family of large enveloped double-stranded DNA viruses that replicate entirely in the cytoplasm ...of virus particles (8), cell entry (20), and ...
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Localization and fine structure of a vaccinia virus gene encoding an envelope antigen.
... RNA from vaccinia virus-infected cells was selected by hybridization to cloned HindIII restriction fragments of the vaccinia virus genome.. Five nitrocellulose membranes containing the i[r] ...
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Vaccinia Virus WR53.5/F14.5 Protein Is a New Component of Intracellular Mature Virus and Is Important for Calcium-Independent Cell Adhesion and Vaccinia Virus Virulence in Mice
... variola virus, the causative agent of smallpox disease in humans ...monkeypox virus produces a smallpox-like disease, and molluscum conta- giosum virus causes relatively benign wart-like lesions (7, ...
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