Vector integration
Enhancers Are Major Targets for Murine Leukemia Virus Vector Integration
... unselected integration sites for an MLV vector are also valuable for the gene therapy ...the integration site prefer- ences of an MLV vector in CD34 ⫹ ...the integration sites from our ...
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Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2locus
... (MLV) vector had integrated proximal to the promoter of an oncogene involved in T-cell acute lym- phoblastic leukemia, LIM-only protein 2 (LMO2), resulting in aberrant ...ral vector integration near ...
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Lentiviral vector integration in the human genome induces alternative splicing and generates aberrant transcripts
... cells. Integration into transcribed genes also has the potential to deregulate gene expression at the posttranscriptional level by interfering with splicing and polyadenylation of primary tran- ...retroviral ...
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Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver
... rAAV2 integration may not disrupt genes but in some cases may allow proviruses to drive flanking cellular genes, similar to retroviral long ter- minal ...rAAV2 integration at a frequency of ...
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Adeno-associated virus vector integration junctions.
... integrated vector proviruses has not previously been analyzed at the DNA sequence ...the integration of wild-type AAV have identified a common site-specific integration locus on human chromosome 19; ...
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VISPA: a computational pipeline for the identification and analysis of genomic vector integration sites
... were vector-only sequences (21% on average); or (3) repetitive elements that could not be univocally mapped to the reference genome (13% on ...same integration event (the IS merging step), the number of ...
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Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID X1 gene therapy
... common integration sites (CISs). The highly significant incidence of CISs in circulating T cells and the nature of their locations indicate that insertion in many gene loci has an influence on cell engraftment, ...
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Chromosomal Integration of Adenoviral Vector DNA In Vivo
... chromosomal integration of AAV, retrovirus, Sleeping Beauty transposon, and plasmid DNA in hepatocytes (13, 25, 27, ...Ad vector with the E1 deletion (30) into FAH ⫺/⫺ mice, the devel- opment of FAH ⫹ ...
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Insertional Gene Activation by Lentiviral and Gammaretroviral Vectors
... estimated vector copy numbers and the number of integration sites in each mutant that were cloned by inverse ...integrated vector copies were demonstrated by ...by vector integration in ...
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The Molecular Basis of Multiple Vector Insertion by Gene Targeting in Mammalian Cells
... in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of ...the vector-borne and chromosomal Cm sequences at the recombinant locus to be ...
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A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1
... Figure 2 Rescue of the integrated plasmid and determination of the sequence of attB. The structure of the rescued plasmid, pMS98R, by digestion of genomic DNA from an S. coelicolor J1929 pMS98 exconjugant with StuI and ...
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A new vector system for targeted integration and overexpression of genes in the crop pathogen Fusarium solani
... streaked on selective PDA of which six showed uninhib- ited growth. Subsequent diagnostic colony PCR showed furthermore that the T-DNA had correctly recombined into the integration site in all colonies tested ...
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External Debt and Economic Growth in Ghana: A Co integration and a Vector Error Correction Analysis
... from 1990 to 2013 for 39 sub-Saharan African countries to check for the relationship between external debt and economic growth. The paper finds a negative impact between debt and growth. In addition, the categorization ...
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Pappou, Emmanouil (2012): The role of IKK2 in TNF-alpha-induced migration and proliferation of human mesenchymal stem cells. Dissertation, LMU München: Medizinische Fakultät
... The vector contains the human CMV immediate early promoter to allow high-level, constitutive expression of the gene of interest in mammalian cells (Boshart, Weber et ...the vector (Dull, Zufferey et al. ...
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Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
... Production and selection of EBV recombinant vectors. The C17 cell lines (3) propagating the p220.2 EBV shuttle vectors (9, 44) carrying segments of AAVS1 DNA, designated C17-p220.2(AAVS1 kb 0-8.2), C17-p220.2(AAVS1 kb ...
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Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... during integration and/or after integration, the AAV ITRs are cleaved by the DNAPKcs-Artemis complex, resulting in local chromosomal changes at the site of integration that may lead to our observed ...
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Foamy Virus Vector Carries a Strong Insulator in Its Long Terminal Repeat Which Reduces Its Genotoxic Potential
... ABSTRACT Strong viral enhancers in gammaretrovirus vectors have caused cellular proto-oncogene activation and leukemia, necessitating the use of cellular promoters in “enhancerless” self-inactivating integrating vectors. ...
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Alpharetroviral Vectors: From a Cancer-Causing Agent to a Useful Tool for Human Gene Therapy
... retroviral vector genotoxicity described to ...single integration may affect more than one gene and induce transcriptional deregulation by more than one mechanism simultaneously ...optimized vector ...
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Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes
... site-specific integration into the Y chromosome has revolutionised our ability to separate males from females, which was previously based on inefficient procedures such as differ- ences in the size of ...
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Odometry and insect navigation
... in vector summation, a navigation error of 180° – leading the animal in the direction opposite of home – may be reached after 1000 strides with a miniscule systematic angular error of ...
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