Viability assay
Development and Evaluation of a Molecular Viability Assay for Pneumocystis carinii
... Determination of organism viability does not prove infec- tiousness. However, determining where, when, and under what circumstances viable P. carinii occurs in the environment will help to narrow down the list of ...
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Luminescent multiplex viability assay for Trypanosoma brucei gambiense
... multiplex viability assay (LMVA) for in vitro compound screening on bloodstream form ...EnduRen assay. In the EnduRen assay, only the most hygromycin resistant population allowed fast ...
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Conidial Viability Assay for Rapid Susceptibility Testing of Aspergillus Species
... hyphal viability after exposure to triazole antifungals with the FUN-1 dye ...conidial viability. The FUN-1-based FCM assay requires just 4 h and can be performed from the primary culture of the ...
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Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)
... metabolic viability assay was used to investi- gate the effect of WPC exposure on A549 cells after 24, 48, 72 and 96 ...cell viability by WPC (4 mg/ml) was observed with the great- est effect at 96 h ...
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Original Article Alendronate stimulates osteoblast differentiation through PKA-STAT3 and STAT1 in an osteoporosis rat model
... Cell viability assay, cell differentiation assay, gene silencing, and Western blot techniques were used to analyze the effects of Alendronate on osteoclasts and osteoblasts activity and PKA-STAT3 and ...
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Original Article FL118 inhibited the proliferation of colorectal carcinoma cells via upregulating CDC73
... The human colorectal cancer cells HCT-116, LOVO, SW480 were kindly provided by Institute for Translation Medicine of Qingdao University (Shandong, China). LOVO was cultured in RPMI- 1640 with 10% FBS (PAN), HCT-116 and ...
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Effect of curcuma longa loaded lauric acid lipid vehicle against propionibacterium acnes and its cytotoxic effects on pbmc and hacat
... Cell viability assay on Peripheral Blood Mononuclear Cells by Dye Exclusion Method and the Cytotoxicity on HaCaT cell line by MTT assay were studied on the Curcuma longa loaded ...
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The positive feedback loop between ILF3 and lncRNA ILF3-AS1 promotes melanoma proliferation, migration, and invasion
... ity assay and the ethynyl deoxyuridine (EdU) incorporation ...cell viability assay, indicated melanoma cells were plated into 96-well plates with 3,000 ...Cell Viability Assay (Promega ...
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Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems
... Development of cancer is often related with multiple mutations and hence it can be hypothesised that DTE could have anticancer activity as it was found to be a po- tent antimutagenic substance. Earlier it has been ...
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Effects of Indocyanine green on cultured retinal ganglion cells in vitro
... After 1 and 5-minute incubation at all ICG concentra- tions, the cells continued to show similar structural fea- tures compared to controls. However, there was a significant morphological variation after 15 and 60- ...
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Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib
... In breast cancer cells, the activation of ER can continuously enhance the expression of cyclin D1 through directly binding to the promoter, which encodes the cyclin D1 isoform [20]. Furthermore, E2F, which is involved in ...
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Antimicrobial and TNF-alpha Inhibitory Activity of Barleria prionitis and Barleria grandiflora: A Comparative Study
... The result of the study shows the significant effectiveness of ethanolic extracts; hence, for further study of cell viability assay, cytokine secretion and inhibition assay ethanolic extracts of both ...
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Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery
... cell viability assay to assess the cell viability of mouse embryonic fibroblasts (MEFs) exposed to POPC liposomes at 50, 75 and 100 μM and decorated with different molar ratios of the lipid–peptide ...
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Epithelial expression of TLR4 is modulated in COPD and by steroids, salmeterol and cigarette smoke
... placed in serum free medium or cigarette smoke condensates for 4 hours. Cigarette smoke condensates were prepared as described in the methods and numbers correspond to serial dilutions of the initial cigarette smoke ...
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Downregulation of miR-483-5p decreases hypoxia-induced injury in human cardiomyocytes by targeting MAPK3
... In line with impaired cell viability, the number of apoptotic cells significantly in- creased in the hypoxia group compared with the normoxia group (Fig. 2b). We also an- alyzed whether hypoxia induced oxidative ...
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In vitro properties of chitosan nanoparticles induce apoptosis in human lymphoma SUDHL 4 cell line
... MTT assay, MMP, agarose gel electrophoresis of DNA, flow cytometry and elec- tron microscopy were used with treatment of differ- ent concentrations of CH-NPs (25, 50, 75, 100 µg/ml) at different time ...
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Bacterial viability on chemically modified silicon nanowire arrays
... Bacterial viability of cells in the planktonic state and attached to surfaces was investigated and the individual effect of each functionality is described ...
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<p>MiR-1299 Impedes the Progression of Non-Small-Cell Lung Cancer Through EGFR/PI3K/AKT Signaling Pathway</p>
... chamber assay showed the invasions of miR-1299-overex- pressed A549 and H1975 cells were signi fi cantly inhibited, while the invasions were promoted after transfection of miR- 1299 inhibitor (P < ...
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Preparation of Pre Confluent Retinal Cells Increases Graft Viability In Vitro and In Vivo: A Mouse Model
... We sought to ascertain if culturing DH01 cells post-confluence or the process of preparing cells for transplantation (TC) induced cell death, and more specifically apoptosis. We cultured DH01 cells to pre-confluent (0.56 ...
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TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion
... Cell lysates were extracted with RIPA cell lysis buffer, and the protein concentration in the lysates was quantified using an enhanced bicinchoninic acid protein assay kit (Thermo Fisher Scientific). Protein ...
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