Viable But Not Culturable
The Molecularly Crowded Cytoplasm of Bacterial Cells: Dividing Cells Contrasted with Viable but Non-culturable (VBNC) Bacterial Cells
... The dynamic cytoplasm normally present in rapidly growing bacterial cells allows them to complete their cell cycles and divide (see Table 1 and Figure 1). A contrasting cytoplasmic state (see Table 2) is present in ...
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Desiccation induces viable but Non-Culturable cells in Sinorhizobium meliloti 1021
... as viable (42-54%) based on the Baclight live/dead stain did not change significantly over ...of viable cells to form colonies shows (i) an underestimation of the survival of rhizobial cells using plating ...
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Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy
... Fig. 2 Dimension and fraction of viable but non-culturable (VBNC) cells. a Average length of VBNC cells (green squares), alongside persister (blue circles), susceptible non-lysed (black upward triangles), ...
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Structural and proteomic changes in viable but non-culturable vibrio cholerae
... In between seasonal outbreaks of cholera, V. cholerae resides in environmental water bodies. In environmental samples, plate counts frequently resulted in lower cell numbers of the pandemic V. cholerae O1 serotype than ...
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Detection of viable but non culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study
... Non-Culturable (VBNC) forms [4, 5] – promotes pathogen survival and infection persistence. Routine culture-based diagnosis of P. aeruginosa lung infection may fail to detect these bacterial populations in CF ...
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Viable but Nonculturable Bacteria Are Present in Mouse and Human Urine Specimens
... of viable but nonculturable bacteria in human clean-catch and mouse bladder-isolated urine specimens was ...investigated. Viable but nonculturable bacteria are alive but do not give rise to visible growth ...
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Does discovery of differentially culturable M tuberculosis really demand a new treatment paradigm? Longitudinal analysis of DNA clearance from sputum
... Our analysis has several limitations. First, there is un- certainty around our assumption that 99% of DNA is from viable Mtb prior to treatment. In sensitivity ana- lyses described in Additional file 2, we tested ...
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The effects of pesticides on the diversity of culturable soil bacteria
... Fig. 1 Comparison of viable counts on different media. Pairwise comparisons of counts on the different media are shown, standard errors (not shown as error bars would be obscured by symbols) were calculated from ...
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Culture Age on Evaluation of Electrically Active Magnetic Nanoparticles as Accurate and Efficient Microbial Extraction Tools
... This cumulative total of 323 repetitive broth challenges yielded statistically significant extraction and culture detection by capture efficiency. Monitoring the age of the inoculating culture during initial challenges ...
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The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
... of culturable cells, viable but non-culturable cells (VBNC) and dead cells were generated when freeze-dried cells were kept at − 21 and 4°C for 12 months whereas higher storage temperature (35°C) ...
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Approaches for diversity analysis of cultivable and non cultivable bacteriain real soil
... all of these methods provide very accurate results in the description of microbial diversity. However, major disadvantage of these methods is the inability to detect so-called non-cultivable (non-culturable) ...
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ANTHELMINTIC EFFECTS OF EXTRACTS OF SOME INDIGENOUSLY ISOLATED CULTURABLE CYANOBACTERIAL SPECIES
... Culturable strains were selected for cultivation of suitable cell mass. Methanolic and aqueous extracts were obtained from dried cell mass at 40°c temperature. These extracts were used for anthelmintic activities ...
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Sponge associated bacteria of Lakshadweep coral reefs, India: resource for extracellular hydrolytic enzymes
... Sponges were washed thoroughly with autoclaved; filter sterilized (0.22 µm-pore size filter) seawater until visibly free of debris and sediments. They were then homoge- nized using sterile mortar and pestle. Serially ...
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Isolation, cytotoxic activity and phylogenetic analysis of Bacillus sp bacteria associated with the red sea sponge Amphimedon ochracea
... The recovery of bacterial strains with cytotoxic activity suggests that marine invertebrates remain a rich source for the isolation of culturable isolates capable of producing novel bioa[r] ...
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Acute intestinal obstruction in adults: An analytical study of 75 cases
... Bowel Viability Complications Resection and Anastomosis with Herniorraphy Adhesiolysis Reduction and Herniorraphy Laparotomy and Reduction Gangrene Nil Viable Viable Viable Nil Nil Nil R[r] ...
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Beneficial but not sufficient: effects of condom packaging instructions on condom use skills
... More specifically, the one-third of a MOCUS step difference observed for the aggregate efficacy measure can be attributed to the increased proportion of packaging instructi[r] ...
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Fungal root endophytes of tomato from Kenya and their nematode biocontrol potential
... Abstract The significance of fungal endophytes in African agriculture, particularly Kenya, has not been well investigat- ed. Therefore, the objective of the present work was isolation, multi-gene phylogenetic ...
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Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing
... both groups could synthesize most essential amino acids (such as glutamine, glutamate, lysine, and threonine biosyn- thesis) and B vitamin-related enzyme cofactors (folate, thiamine, flavodoxin, and pyridoxin) de novo. ...
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Microbial endophytes that live within seeds of two
... Culturable bacteria isolated from seeds and seedlings of both cultivars of tomato were similar. 458[r] ...
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Experimental evidence for the co evolution of hominin tool making teaching and language
... Variable Total quality Number of viable flakes Proportion of flakes that are viable Viable flakes per minute Proportion of core knapped Probability of a viable flake per hit.. Estimated [r] ...
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